Glucose repression of anthracycline formation in Streptomyces peucetius var. caesius

Escalante, Laura; Ramos, Itzel; Imriskova, Iveta; Langley, Elizabeth; Sánchez, Sergio

doi:10.1007/s002530051562

Cited by 32 publications

(26 citation statements)

References 14 publications

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“…136 In addition to presenting CCR insensitivity and low Glk activity, Dog R mutants also show difficulties in transporting glucose. 93 As previously reported for a Dog R mutant from S. coelicolor, 125 a partial reversion of the Dog R phenotype is observed when transforming the mutant with glkA. However, a complete reversion of this phenotype is obtained when the mutant is transformed with the sco2127 gene alone.…”

Section: Streptomycessupporting

confidence: 51%

“…In addition, in a resting cell system containing protein-synthesis inhibitors, anthracycline formation is not affected by high glucose concentrations, suggesting a repressive rather than an inhibitory effect as the possible regulatory mechanism. 93 Regulation of antibiotic production by the carbon source S Sánchez et al Nystatin belongs to the family of aminoglycosides. When Streptomyces noursei American Type Culture Collection 11455 is supplied with glucose as the carbon source, although high nystatin titers are achieved because of high biomass concentration, glucose tends to negatively influence nystatin production.…”

Section: Streptomycesmentioning

confidence: 99%

“…For instance, actinorhodin production is sensitive to glucose concentrations in the range of 100 mM; however, higher concentrations are necessary to repress doxorubicin production. 93 Mechanism of CCR in Streptomyces CCR control by PTS. The mechanism for CCR in Streptomyces is not clearly understood and there is probably more than one mechanism involved.…”

Section: Streptomycesmentioning

confidence: 99%

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Carbon source regulation of antibiotic production

et al. 2010

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Section: Streptomycessupporting

confidence: 51%

Section: Streptomycesmentioning

confidence: 99%

Section: Streptomycesmentioning

confidence: 99%

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Carbon source regulation of antibiotic production

et al. 2010

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“…Nevertheless, the uptake of fructose will lower the amount of PϳEI and PϳHPr and consequently will also lower the amount of PϳEIIA Glc . Although the main uptake system for glucose in S. coelicolor is GlcP, a non-PTS permease of the major facilitator superfamily (911), CCR by glucose was observed (21,217,530,912). Glucose kinase (443,911) and the accumulation of glycolytic intermediates (694) are implicated in mediating the effect.…”

Section: Regulation By Eiia Glc -Like Proteinsmentioning

confidence: 99%

How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bacteria

Deutscher

Francke

Postma

2006

Microbiol Mol Biol Rev

1,203

769

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SUMMARY The phosphoenolpyruvate(PEP):carbohydrate phosphotransferase system (PTS) is found only in bacteria, where it catalyzes the transport and phosphorylation of numerous monosaccharides, disaccharides, amino sugars, polyols, and other sugar derivatives. To carry out its catalytic function in sugar transport and phosphorylation, the PTS uses PEP as an energy source and phosphoryl donor. The phosphoryl group of PEP is usually transferred via four distinct proteins (domains) to the transported sugar bound to the respective membrane component(s) (EIIC and EIID) of the PTS. The organization of the PTS as a four-step phosphoryl transfer system, in which all P derivatives exhibit similar energy (phosphorylation occurs at histidyl or cysteyl residues), is surprising, as a single protein (or domain) coupling energy transfer and sugar phosphorylation would be sufficient for PTS function. A possible explanation for the complexity of the PTS was provided by the discovery that the PTS also carries out numerous regulatory functions. Depending on their phosphorylation state, the four proteins (domains) forming the PTS phosphorylation cascade (EI, HPr, EIIA, and EIIB) can phosphorylate or interact with numerous non-PTS proteins and thereby regulate their activity. In addition, in certain bacteria, one of the PTS components (HPr) is phosphorylated by ATP at a seryl residue, which increases the complexity of PTS-mediated regulation. In this review, we try to summarize the known protein phosphorylation-related regulatory functions of the PTS. As we shall see, the PTS regulation network not only controls carbohydrate uptake and metabolism but also interferes with the utilization of nitrogen and phosphorus and the virulence of certain pathogens.

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“…However, like many other secondary metabolites, OTC biosynthesis is affected by the carbon source concentration. When glucose is added to the culture medium at higher concentration, it shows a negative influence on antibiotic production due to carbon catabolite repression (CCR) as in many cases such in anthrocycline production by S. peucetius (Escalante et al 1999), cephamycin C by S. clavuligerus (Demain and Fang 1995), and retamycin by S. olindensis (Inoue et al 2007). In addition, the overflow of glucose in the primary metabolic pathway in streptomycetes promotes organic acid production in the culture medium (Dekleva and Stroh 1987).…”

Section: Introductionmentioning

confidence: 99%