Glucose requirement for DNA breakdown resulting from treatment of Bacillus subtilis with ultraviolet radiation or methyl methanesulfonate

Searashi, T.; Strauss, Bernard S.

doi:10.1016/0027-5107(67)90032-2

Cited by 6 publications

(3 citation statements)

References 8 publications

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“…Since the composition of the plating medium did not influence survival and since essentially the only difference between liquid and solid media is the extent of aeration, it might be that cells vigorously aerated in suspension exhibit an enhanced rate of ATP production and that an unusually high level of ATP is necessary to repair the damaged DNA before some critical stage of the division cycle is reached. In this connection, it was shown that glucose was required for the repair of DNA after treatment of bacteria with ultraviolet light and methyl methanesulfonate (21,23).…”

Section: Discussionmentioning

confidence: 99%

Recovery from N -Hydroxyurethan-Induced Death

Mullinix

Rosenkranz

1971

J Bacteriol

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Bacteria (Escherichia coli) can recover from the lethal action of N-hydroxyurethan when they are incubated in drug-free liquid medium. This recovery, which is dependent upon energy metabolism, does not occur on solid medium. Recovery is accompanied by repair of the cellular deoxyribonucleic acid (DNA). A bacterial mutant deficient in DNA polymerase was extremely sensitive to the lethal action of hydroxyurethan. Data are presented which support the concept that DNA replication and DNA repair are mediated by different enzymes and that only the former process is inhibited by hydroxyurea.

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Section: Discussionmentioning

confidence: 99%

Recovery from N -Hydroxyurethan-Induced Death

Mullinix

Rosenkranz

1971

J Bacteriol

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“…Since uvrcells, which fail to excise pyrimidine dimers, are sensitive to UV but not to MMS (38), the defect in JBI-49(59) appears to be in one of the later steps in the excision-repair model sequence. The rate of DNA degradation after UV or MMS treatment is even greater for JB1-49(59) than for the wild type (39,41). As this breakdown requires metabolism for its maximum extent, it probably represents not simple autolysis of nonviable cells, but rather specific exonuclease activity.…”

Section: Jbi-49(59) Has Several Interesting Biologicalmentioning

confidence: 99%

Altered Deoxyribonucleic Acid Polymerase Activity in a Methyl Methanesulfonate-Sensitive Mutant of Bacillus subtilis

et al. 1971

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Of six deoxyribonucleic acid repair mutants of Bacillus subtilis assayed for deoxyribonucleic acid polymerase, only the methyl methanesulfonate-sensitive and ultraviolet light-sensitive mutant JBI-49(59) has impaired polymerase activity. Extracts prepared by sonic treatment or gentle lysis had about 10% of the wild-type activity with poly d(A-T), an alternating copolymer of deoxyadenylate and deoxythymidylate, used as template. The sensitivity to methyl methanesulfonate and ultraviolet light and the low level of polymerase activity transformed and reverted together, indicating that the two characteristics are a pleiotropic manifestation of a single mutation. Mixed extract and kinetic experiments mitigated against an altered nuclease activity as the enzymatic consequence of the mutation. Also, the mutant and wild type activities were stimulated equally by Escherichia coli exonuclease III. The residual activity in the mutant showed several differences from the wild-type activity: it purified differently, was more sensitive to sulfhydryl reagents, and displayed a different template specificity. We tentatively conclude that either the mutation in JBI-49(59) has introduced a qualitative as well as a quantitative change in the polymerase or the wild type contains two distinct polymerases, one of which is missing in the mutant.

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“…No similar finding has been made with the MMSsensitive strains. The strain of B. subtilis isolated by Searashi degrades its DNA after MMS treatment (Searashi & Strauss, 1967), but the mechanism is not known. Numerous other MMS-sensitive strains have been isolated (Mahler, 1966;Howard-Flanders & Boyce, 1966), but in no case is the nature of the metabolic defect known.…”

Section: Repair Of Alkylated D N Amentioning

confidence: 99%