Glucose-Responsive Regulators of Gene Expression in <i>Saccharomyces cerevisiae</i> Function at the Nuclear Periphery via a Reverse Recruitment Mechanism

Sarma, Nayan J.; Haley, Terry; Barbara, Kellie E.; Buford, Thomas D.; Willis, Kristine A.; Santangelo, George M.

doi:10.1534/genetics.106.068932

Cited by 51 publications

(69 citation statements)

References 40 publications

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“…A role for Nup2p in the perinuclear localization of actively transcribing GAL loci has previously been described (Schmid et al 2006). The perinuclear localization of the GAL transcription sites, as well as those for KHK1 and INO1, has also been correlated with an increase in the transcription of these genes (Brickner and Walter 2004;Schmid et al 2006;Taddei et al 2006;Sarma et al 2007), lending support to the view that the NPC basket affects the process of gene expression.…”

Section: Introductionmentioning

confidence: 61%

A nucleoporin, Nup60p, affects the nuclear and cytoplasmic localization of ASH1 mRNA in S. cerevisiae

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2010

RNA

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The biogenesis of a localization-competent mRNP begins in the nucleus. It is thought that the coordinated action of nuclear and cytoplasmic components of the localization machinery is required for the efficient export and subsequent subcellular localization of these mRNAs in the cytoplasm. Using quantitative poly(A)+ and transcript-specific fluorescent in situ hybridization, we analyzed different nonessential nucleoporins and nuclear pore-associated proteins for their potential role in mRNA export and localization. We found that Nup60p, a nuclear pore protein located on the nucleoplasmic side of the nuclear pore complex, was required for the mRNA localization pathway. In a Dnup60 background, localized mRNAs were preferentially retained within the nucleus compared to nonlocalized transcripts. However, the export block was only partial and some transcripts could still reach the cytoplasm. Importantly, downstream processes were also affected. Localization of ASH1 and IST2 mRNAs to the bud was impaired in the Dnup60 background, suggesting that the assembly of a localization competent mRNP (''locasome'') was inhibited when NUP60 was deleted. These results demonstrate transcript specificity of a nuclear mRNA retention defect and identify a specific nucleoporin as a functional component of the localization pathway in budding yeast.

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Section: Introductionmentioning

confidence: 61%

A nucleoporin, Nup60p, affects the nuclear and cytoplasmic localization of ASH1 mRNA in S. cerevisiae

Powrie

Zenklusen

Singer³

2010

RNA

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“…Nup42p could also facilitate association of the NPC with target genes. Several yeast genes associate with the nuclear pore upon induction (Brickner and Walter 2004;Casolari et al 2004Casolari et al , 2005Dieppois et al 2006;Schmid et al 2006;Taddei et al 2006;Brickner et al 2007;Sarma et al 2007;Tan-Wong et al 2009), which may promote transcriptional looping and/or couple transcription and mRNA export from the nucleus (Brickner 2009;Hampsey et al 2011). Upon initial inositol starvation, INO1 translocation to the nuclear periphery is dependent on Nup42p (Ahmed et al 2010;Light et al 2010); while initial translocation is independent of Nup100p [but perhaps amplified in its absence (Ahmed et al 2010)], Nup100p is required for persistent peripheral localization and transcriptional memory of INO1 after inositol repletion .…”

Section: Discussionmentioning

confidence: 99%

Cellular Memory of Acquired Stress Resistance inSaccharomyces cerevisiae

et al. 2012

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Cellular memory of past experiences has been observed in several organisms and across a variety of experiences, including bacteria "remembering" prior nutritional status and amoeba "learning" to anticipate future environmental conditions. Here, we show that Saccharomyces cerevisiae maintains a multifaceted memory of prior stress exposure. We previously demonstrated that yeast cells exposed to a mild dose of salt acquire subsequent tolerance to severe doses of H 2 O 2 . We set out to characterize the retention of acquired tolerance and in the process uncovered two distinct aspects of cellular memory. First, we found that H 2 O 2 resistance persisted for four to five generations after cells were removed from the prior salt treatment and was transmitted to daughter cells that never directly experienced the pretreatment. Maintenance of this memory did not require nascent protein synthesis after the initial salt pretreatment, but rather required long-lived cytosolic catalase Ctt1p that was synthesized during salt exposure and then distributed to daughter cells during subsequent cell divisions. In addition to and separable from the memory of H 2 O 2 resistance, these cells also displayed a faster gene-expression response to subsequent stress at .1000 genes, representing transcriptional memory. The faster gene-expression response requires the nuclear pore component Nup42p and serves an important function by facilitating faster reacquisition of H 2 O 2 tolerance after a second cycle of salt exposure. Memory of prior stress exposure likely provides a significant advantage to microbial populations living in ever-changing environments. N ATURAL environments are complex and often vary significantly in space and time, posing challenges for the organisms living within them. Single-cell organisms are particularly vulnerable, since variation in external conditions can directly impact internal homeostasis. Therefore, preparing for environmental change after early signs of fluctuation would present a significant advantage for cells growing in the wild. Indeed, many organisms can become tolerant to severe stress after an initial mild pretreatment with the same or a different stressor. This response, termed "acquired stress resistance," has been observed in microbes such as bacteria and yeast as well as in multicellular organisms including worms, plants, mammals, and even humans (Lu et al. 1993;Davies et al. 1995;Lewis et al. 1995;Lou and Yousef 1997;Swan and Watson 1999;Chi and Arneborg 2000;Schenk et al. 2000;Durrant and Dong 2004;Kandror et al. 2004;Scholz et al. 2005;Hecker et al. 2007;Kensler et al. 2007;Matsumoto et al. 2007). The conservation of this response suggests that it plays an important role in surviving environmental stress in diverse species.We previously conducted a systematic analysis of acquired stress resistance in Saccharomyces cerevisiae and found that the response is common, but not universal, for all pairs of stress treatments (Berry and Gasch 2008). The level and duration of mild-stress pretreatme...

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“…It has also been proposed that gene localization within the nucleus is sometimes regulated in response to regulatory signals (Menon et al 2005;Sarma et al 2007). According to this model, genes and their associated activators move to the nuclear periphery where they encounter the transcriptional machinery at nuclear pore complexes.…”

Section: Functions Of Tf-dna Bindingmentioning

confidence: 99%

Transcriptional Regulation inSaccharomyces cerevisiae: Transcription Factor Regulation and Function, Mechanisms of Initiation, and Roles of Activators and Coactivators

2011

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Here we review recent advances in understanding the regulation of mRNA synthesis in Saccharomyces cerevisiae. Many fundamental gene regulatory mechanisms have been conserved in all eukaryotes, and budding yeast has been at the forefront in the discovery and dissection of these conserved mechanisms. Topics covered include upstream activation sequence and promoter structure, transcription factor classification, and examples of regulated transcription factor activity. We also examine advances in understanding the RNA polymerase II transcription machinery, conserved coactivator complexes, transcription activation domains, and the cooperation of these factors in gene regulatory mechanisms. TABLE OF CONTENTS Abstract 705Introduction 706

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Glucose-Responsive Regulators of Gene Expression in Saccharomyces cerevisiae Function at the Nuclear Periphery via a Reverse Recruitment Mechanism

Cited by 51 publications

References 40 publications

A nucleoporin, Nup60p, affects the nuclear and cytoplasmic localization of ASH1 mRNA in S. cerevisiae

A nucleoporin, Nup60p, affects the nuclear and cytoplasmic localization of ASH1 mRNA in S. cerevisiae

Cellular Memory of Acquired Stress Resistance inSaccharomyces cerevisiae

Transcriptional Regulation inSaccharomyces cerevisiae: Transcription Factor Regulation and Function, Mechanisms of Initiation, and Roles of Activators and Coactivators

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