Glucose-Sensitive Microcapsules from Glutaraldehyde Cross-Linked Hemoglobin and Glucose Oxidase

Qi, Wei; Yan, Xuehai; Li, Duan; Cui, Yue; Yang, Yang; Li, Junbai

doi:10.1021/bm801502r

Cited by 110 publications

(79 citation statements)

References 35 publications

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“…They found that the (GOx-hemoglobin)5 microcapsules became permeable to dextran in the presence of 100 mM glucose, although dextran was impermeable without glucose. 122 They also prepared GOx-catalase LbL layers on the surface of an insulin microcrystal, and found that the dissolution rate of insulin increased in response to glucose. 123 The effects of glucose were explained by the local pH changes induced by the GOx-catalyzed reaction of glucose.…”

Section: ·2 Controlled Release From Lbl Microcapsulesmentioning

confidence: 99%

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

2012

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We present an overview of the preparation and properties of layer-by-layer (LbL) deposited thin films and microcapsules in relation to their use in the development of biosensors and controlled release systems. Enzyme biosensors can be constructed by immobilizing enzymes on the surface of electrodes by LbL deposition without loss of their catalytic activity. In addition to synthetic polymers, binding proteins, such as avidin and lectin, are also used for constructing LbL films through avidin-biotin and lectin-sugar interactions. The performance characteristics of LbL film-based biosensors can be tuned by controlling the number of layers and by the choice of film components. The permeability of polyelectrolyte LbL films to ions and molecules is discussed in relation to the use of the films for eliminating interference in biosensors. The possible use of polysaccharide LbL film-coated electrodes for the construction of biosensors is highlighted, and examples of LbL film-and microcapsule-based optical sensors are described. We then focus on the use of LbL films and microcapsules as vehicles for controlled release, in particular on recent progress in the controlled release of insulin from LbL films and microcapsules. LbL films composed of insulin and polymers are sensitive to environmental pH, and release insulin in response to pH changes, suggesting that insulin LbL films can be used in the development of orally administered insulin. The construction of glucose-dependent insulin release systems using LbL insulin microcapsules functionalized with phenylboronic acid, lectin, and glucose oxidase is also examined.

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Section: ·2 Controlled Release From Lbl Microcapsulesmentioning

confidence: 99%

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

2012

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“…Polymer containers based on the LbL technique have recently attracted high interest for a variety of different applications, ranging from drug delivery systems and targeted gene therapy to biosensor devices. [19,20] To date, capsules have been made of synthetic and biodegradable polyelectrolytes, [21,22] comprising natural molecules such as oligonucleotides [23] and proteins, [24,25] which demonstrates the high versatility of LbL assembly. Using standard LbL preparation techniques [18] and employing supercharged proteins E57 and K48 as building blocks, we generated capsules exhibiting the following wall structure: (DEXS/pARG)(E57/K48) 3 (E57/K48 AF488 )E57, where DEXS denotes dextran sulfate and pARG poly-(L-arginine), two charged biodegradable polymers made from naturally occurring monomers (see Supporting Information).…”

Section: Capsule Preparation and Characterizationmentioning

confidence: 99%

De Novo Design of Supercharged, Unfolded Protein Polymers, and Their Assembly into Supramolecular Aggregates

Kolbe

Mercato

Abbasi

et al. 2010

Macromol. Rapid Commun.

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Here we report for the first time the design and expression of highly charged, unfolded protein polymers based on elastin‐like peptides (ELPs). Positively and negatively charged variants were achieved by introducing lysine and glutamic acid residues, respectively, within the repetitive pentapeptide units. Subsequently it was demonstrated that the monodisperse protein polyelectrolytes with precisely defined amino acid compositions, sequences, and stereochemistries can be transferred into superstructures exploiting their electrostatic interactions. Hollow capsules were assembled from oppositely charged protein chains by using the layer‐by‐layer technique. The structures of the capsules were analyzed by various microscopy techniques revealing the fabrication of multilayer containers. Due to their low toxicity in comparison to other polyelectrolytes, supercharged ELPs are appealing candidates for the construction of electrostatically induced scaffolds in biomedicine. magnified image

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“…Considerable effort has been devoted to achieving this goal via incorporation of functional materials or nanoparticles into the microcapsule shell to control the release of the contents of the microcapsules through the application of an external stimulus, 1-3 such as pH, 4-7 temperature, [8][9][10] light, [11][12][13] ultrasound, [14][15][16][17] glucose 18,19 and magnetic field, [20][21][22] at the appropriate time. Significant progress has been achieved to develop such controlled release methods; however, methods with better controllability are still in demand.…”

Section: Introductionmentioning

confidence: 99%

Bio-inspired controlled release through compression–relaxation cycles of microcapsules

et al. 2015

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The aim of microencapsulation is to achieve controllable and sustainable release at the desired site. Despite the many methods developed in recent years, there is still a need to advance the strategies for higher controllability and scalability. Taking inspiration from the heart, which pumps blood by continual contraction of muscles, we demonstrate a novel releasing method, in which the core release is controlled by the compression-relaxation cycles of microcapsules upon the application of a magnetic field. This idea is realized by embedding Fe 3 O 4 particles into a polymer shell. When the magnetic field is applied, the Fe 3 O 4 particles align along the field direction and stretch the shell, resulting in compression of the microcapsules and release of the core contents; the shape is restored after the field is removed. We demonstrated various release patterns by altering the strength of the magnetic field and the compression frequency. This release method provides repeated and pulsatile delivery, enhanced spreading distance and ejection speed, and is site specific and proactive, providing a new option for controlled release applications.

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Glucose-Sensitive Microcapsules from Glutaraldehyde Cross-Linked Hemoglobin and Glucose Oxidase

Cited by 110 publications

References 35 publications

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

De Novo Design of Supercharged, Unfolded Protein Polymers, and Their Assembly into Supramolecular Aggregates

Bio-inspired controlled release through compression–relaxation cycles of microcapsules

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