Glucose-sensitive neurons in the rat arcuate nucleus contain neuropeptide Y

Muroya, Shinji; Yada, Toshihiko; Shioda, Seiji; Takigawa, Morikuni

doi:10.1016/s0304-3940(99)00185-8

Cited by 185 publications

(138 citation statements)

References 17 publications

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“…The majority of GS neurons in the ARC were recently shown to contain NPY [19], suggesting a role for NPY in glucose sensing. Under conditions of negative energy balance, such as untreated Type I diabetes, the arcuo-PVN NPY-containing pathway seems to be stimulated, resulting in increased peptide and NPY mRNA, which is thought to be associated with hyperphagia in these animals [25,26,29].…”

Section: Discussionmentioning

confidence: 99%

“…After two basal collections of 20 min, tissues were exposed to Krebs containing 1.5 mmol/l glucose, followed by exposure to normal Krebs solution to assess recovery of NPY overflow. The low glucose concentration of 1.5 mmol/l was based on the glucose concentrations reported to elicit changes in nerve activity [19,21]; this is within the range of CNS concentrations experienced during hypoglycaemia. The tissues were challenged 40 min later with Krebs solution containing 45 mmol/l K + (with reduced Na + to maintain osmolarity) to test the viability of the tissues.…”

Section: Methodsmentioning

confidence: 99%

“…The NPY-containing neurons in the ARC have been implicated in feeding [24] and glucosesensing [19]. Furthermore, NPY mRNA and NPYoverflow from several hypothalamic sites was shown to be increased in streptozotocin-induced diabetic rats compared to control rats [25,26].…”

mentioning

confidence: 98%

“…Since the proposal of glucose sensing neurons in the hypothalamus [16], many studies have investigated this question. Effects of systemic glucose on neurons of the lateral hypothalamus (LH), ventromedial nucleus (VMN), paraventricular nucleus (PVN) and arcuate nucleus (ARC) of the hypothalamus, as well as the nucleus tractus solitarius (NTS) of the brainstem have been reported in several mammalian species, including the cat, rat and mouse [17,18,19,20,21]. However, the characteristics of these neurons remain largely unknown.…”

mentioning

confidence: 99%

“…However, the characteristics of these neurons remain largely unknown. Several candidate transmitters are implicated in central glucose sensing, including some amino acids, catecholamines and neuropeptide Y (NPY) [19,22].…”

mentioning

confidence: 99%

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Involvement of neuropeptide Y in glucose sensing in the dorsal hypothalamus of streptozotocin diabetic rats – in vitro and in vivo studies of transmitter release

2002

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Aims. Within the brain, subgroups of neurons respond differently to altered glucose concentrations. Identification of neuropeptide Y in hypothalamic neurons that sense glucose suggests a role for neuropeptide Y in glucose sensing. Using in vitro and in vivo techniques to monitor transmitter release, we investigated whether lowering glucose concentration affects the release of neuropeptide Y from the brain, and whether this process is altered in Type I (insulin-dependent) diabetes mellitus. Methods. Male Sprague-Dawley rats were treated with 48 mg/kg streptozotocin or vehicle intravenously. The effect of reduced glucose on endogenous neuropeptide Y overflow from slices of hypothalamus and medulla incubated in Krebs solution was examined 4 weeks later. The hypothalamus was separated into a dorsal region containing the paraventricular nucleus and a ventral region containing the arcuate nucleus. Results. Streptozotocin-induced diabetes increased basal neuropeptide Y overflow in the dorsal and ventral hypothalamus (p<0.05) but not the medulla. In vitro neuropeptide Y overflow was reduced by low glucose in the dorsal hypothalamus in diabetic, but not in control rats. No effect of reduced glucose was observed in the ventral hypothalamus or medulla. In vivo push-pull studies in the paraventricular nucleus also showed greater neuropeptide Y overflow in diabetic rats relative to control rats (p<0.05). Insulininduced hypoglycaemia induced a decrease in neuropeptide Y overflow in diabetic rats, while an increase was observed in control rats (p<0.05). Conclusion. These region-specific effects of low glucose on neuropeptide Y overflow in diabetic rats support a part for neuropeptide Y in altered glucose sensing in Type I diabetes. [Diabetologia (2002[Diabetologia ( ) 45:1332[Diabetologia ( -1339

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