Glucose suppression of deoxyribose interference in the thiobarbituric acid determination of sialic acid

Smith, Carl H.; Donohue, T. M.; Depper, Rosalyn

doi:10.1016/0003-2697(75)90296-1

Cited by 13 publications

(1 citation statement)

References 7 publications

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“…40 Furthermore, as shown with the resorcinol method, ion exchange resins can be used to isolate sialic acid from compounds that may interfere, but these increase the time to result. 44 One final improvement established that using glucose as an inhibitor can reduce interference from deoxyribose, 45 as it acts as a competitor for oxidation by periodic acid and forms different products, giving formaldehyde and formic acid. Thus, the glucose chromophore formed is entirely different from that of compound 7 and its absorbance does not overlap with that of the sialic acid chromophore giving more accurate results.…”

Section: Periodic Acid/thiobarbituratementioning

confidence: 99%

Assays for the identification and quantification of sialic acids: Challenges, opportunities and future perspectives

Cheeseman

Kuhnle

Spencer

et al. 2021

Bioorganic & Medicinal Chemistry

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Section: Periodic Acid/thiobarbituratementioning

confidence: 99%

Assays for the identification and quantification of sialic acids: Challenges, opportunities and future perspectives

Cheeseman

Kuhnle

Spencer

et al. 2021

Bioorganic & Medicinal Chemistry

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The nonuniform distribution of acidic components on the human placental syncytial trophoblast surface membrane: A cytochemical and analytical study

et al. 1976

Anat. Rec.

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The surface coat of syncytial trophoblast from term human placentas was studied using cytochemical methods (colloidal iron, alcian blue-lanthanum nitrate, dialyzed iron) in coordination with tissue enzyme digestions (trypsin, neuraminidase) and sialic acid analyses. The presence of at least two highly acidic anionic components that contribute significantly to the surface negativity of trophoblast has been demonstrated. The first of these, sialic acid, was removed with neuraminidase. Tissue digestion with this glycosidase was accompanied by a decrease in trophoblast surface staining with colloidal iron, a decrease in tissue sialic acid, and an increase in the concentration of sialic acid in the incubating medium. Results from methylation experiments were consistent with the presence of sialic acid. The second anionic component(s) was identified by removal with trypsin of a glycocalyx constituent that stained with both colloidal iron and lanthanum. After trypsinization, tissue sialic acid levels were not significantly different from control values, and no detectable sialic acid was present in the incubating medium. The identity of this anionic component has not been established. Both sialic acid and nonsialic acid acidic components are distributed in higher density on membrane of microvilli than on intermicrovillous surface membrane. In addition, the sialic acid moieties appear to be clustered in the glycocalyx.

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High α‐amylase activity in the syncytiotrophoblastic cells of first‐trimester human placentas

Fisher

Laine

1983

J of Cellular Biochemistry

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The syncytiotrophoblastic brush border of the human placenta forms the maternal-fetal interface and is an important determinant of placental function. Electron micrographs of fresh brush border preparations isolated from first-trimester human placentas showed membrane vesicles, open-ended microvilli, and numerous glycogen particles. Analysis of the microvillar membranes for several plasma and intracellular membrane markers showed a high degree of purification, comparable to the results reported for the isolation of microvilli from full-term human placentas. The microvillar preparations from first-trimester placentas, however, also contained the enzymes necessary to synthesize and degrade glycogen. The degradation resulted in the accumulation of maltotriose and maltotetraose, apparently due to the action of a liver-type alpha-amylase. The occurrence of this enzyme and the enzymes for synthesizing glycogen in this brush border fraction is probably associated with the necessity for an extremely active glucose transport and liver-like storage system within the fetal tissue at this fetal-maternal membrane interface.

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Glucose suppression of deoxyribose interference in the thiobarbituric acid determination of sialic acid

Cited by 13 publications

References 7 publications

Assays for the identification and quantification of sialic acids: Challenges, opportunities and future perspectives

Assays for the identification and quantification of sialic acids: Challenges, opportunities and future perspectives

The nonuniform distribution of acidic components on the human placental syncytial trophoblast surface membrane: A cytochemical and analytical study

High α‐amylase activity in the syncytiotrophoblastic cells of first‐trimester human placentas

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