1977
DOI: 10.1128/jb.132.2.541-548.1977
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Glucose transport in Streptococcus agalactiae and its inhibition by lactoperoxidase-thiocyanate-hydrogen peroxide

Abstract: Transport of 2-deoxyglucose or glucose in Streptococcus agalactiae was strongly inhibited if the cells were first exposed to a combination of lactoperoxidase-thiocyanate-hydrogen peroxide (LP-complex). The inhibition was completely reversible with dithiothreitol. N-ethylmaleimide and p-chloromercuribenzoate inhibited sugar transport, and the inhibition was also reversible with dithiothreitol. Sodium fluoride also inhibited sugar transport. Glucolysis was completely inhibited, and dithiothreitol completely reve… Show more

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Cited by 44 publications
(24 citation statements)
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“…Glucose transport by toluene-acetonetreated E. coli and S. mutans. E. coli and S. mutans were treated with toluene-acetone as described in reference 9. In agreement with an earlier report (5), we also found it necessary to freeze-thaw S. mutans before toluene-acetone treatment to obtain a PEP response. Prewarmed permeabilized bacteria were added to a reaction mixture containing (final concentration) 25 mM PB, 1 mM MgCl2, 10 mM NaF, and 1 mM PEP.…”
Section: (100%)supporting
confidence: 92%
“…Glucose transport by toluene-acetonetreated E. coli and S. mutans. E. coli and S. mutans were treated with toluene-acetone as described in reference 9. In agreement with an earlier report (5), we also found it necessary to freeze-thaw S. mutans before toluene-acetone treatment to obtain a PEP response. Prewarmed permeabilized bacteria were added to a reaction mixture containing (final concentration) 25 mM PB, 1 mM MgCl2, 10 mM NaF, and 1 mM PEP.…”
Section: (100%)supporting
confidence: 92%
“…Glycolysis has been reported to be blocked at several sites by the products of the lactoperoxidase-thiocyanate-hydrogen peroxide reaction. Transport of glucose can be inhibited (27), as can the activity of hexokinase (2,32) and glyceraldehyde 3-phosphate dehydrogenase (26). This inhibition seems to be due to an oxidation of bacterial sulfhydryl groups to yield sulfenic acid and sulfenyl thiocyanate derivatives (28, 41).…”
Section: Discussionmentioning
confidence: 99%
“…1991). These compounds react with microbial sulfhydryls (Pruitt and Reiter 1985; Reiter and Perraudin 1991) and inhibit various cellular functions, such as glycolysis (Oram and Reiter 1966; Adamson and Pruitt 1981), membrane transport of sugars and amino acids (Hamon and Klebanoff 1973; Mickelson 1977; Loimaranta et al . 1998), and respiration (Thomas and Aune 1978; Law and John 1981).…”
Section: Introductionmentioning
confidence: 99%