Glucose transporter isoform 4 is expressed in the syncytiotrophoblast of first trimester human placenta

Ericsson, Anette; Hamark, Bengt; Powell, Theresa L.; Jansson, Thomas

doi:10.1093/humrep/deh596

Cited by 115 publications

(116 citation statements)

References 44 publications

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“…Three glucose transporters, Glut1, Glut3, and Glut4, have been shown to be present in the placenta [53,98,99]. TGF-b has previously been shown to induce glucose uptake in several models via upregulation of Glut1 [100][101][102][103].…”

Section: Discussionmentioning

confidence: 99%

Id2 Mediates Differentiation of Labyrinthine Placental Progenitor Cell Line, SM10

Selesniemi

Albers

Brown

2016

Stem Cells and Development

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The placenta is an organ that is formed transiently during pregnancy, and appropriate placental development is necessary for fetal survival and growth. Proper differentiation of the labyrinthine layer of the placenta is especially crucial, as it establishes the fetal-maternal interface that is involved in physiological exchange processes. Although previous studies have indicated the importance of inhibitor of differentiation/inhibitor of DNA binding-2 (Id2) helix-loop-helix transcriptional regulator in mediating cell differentiation, the ability of Id2 to regulate differentiation toward the labyrinthine (transport) lineage of the placenta has yet to be determined. In the current study, we have generated labyrinthine trophoblast progenitor cells with increased (SM10-Id2) or decreased (SM10-Id2-shRNA) Id2 expression and determined the effect on TGF-b-induced differentiation. Our Id2 overexpression and knockdown analyses indicate that Id2 mediates TGF-b-induced morphological differentiation of labyrinthine trophoblast cells, as Id2 overexpression prevents differentiation and Id2 knockdown results in differentiation. Thus, our data indicate that Id2 is an important molecular mediator of labyrinthine trophoblast differentiation. An understanding of the regulators of trophoblast progenitor differentiation toward the labyrinthine lineage may offer insights into events governing pregnancy-associated disorders, such as placental insufficiency, fetal growth restriction, and preeclampsia.

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Section: Discussionmentioning

confidence: 99%

Id2 Mediates Differentiation of Labyrinthine Placental Progenitor Cell Line, SM10

Selesniemi

Albers

Brown

2016

Stem Cells and Development

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“…The fetus has very little gluconeogenic ability and therefore relies on the mother for most of its energy supply [41]. Glucose transport in the placenta occurs via facilitated glucose transporters (GLUTs) [42].…”

Section: Fig 6 Ampk Knockdown Inhibits Glucose Transport (A)mentioning

confidence: 99%

“…Glucose transport in the placenta occurs via facilitated glucose transporters (GLUTs) [42]. GLUT isoforms 1, 3, and 4 have been reported to be present in the mouse and human placenta and are thought to play an important role in glucose transport ability [41][42][43]. Glut1 has been found on both the maternal and fetal sides of the rodent placenta and functions to provide glucose for the metabolic needs of these cells [42].…”

Section: Fig 6 Ampk Knockdown Inhibits Glucose Transport (A)mentioning

confidence: 99%

“…Glut1 has been found on both the maternal and fetal sides of the rodent placenta and functions to provide glucose for the metabolic needs of these cells [42]. Glut4 has been difficult to isolate in the rodent placenta but can be found in human syncytiotrophoblast, though its role is currently unclear [41]. The Glut3 transporter is primarily located on the fetal side of the placenta, the labyrinthine layer in rodents, and is used to transport glucose from the mother to the fetus [42].…”

Section: Fig 6 Ampk Knockdown Inhibits Glucose Transport (A)mentioning

confidence: 99%

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AMPK Knockdown in Placental Trophoblast Cells Results in Altered Morphology and Function

Carey

Albers

Doliboa

et al. 2014

Stem Cells and Development

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The placenta is a transient organ that develops upon the initiation of pregnancy and is essential for embryonic development and fetal survival. The rodent placenta consists of distinct lineages and includes cell types that are analogous to those that make up the human placenta. Trophoblast cells within the labyrinth layer, which lies closest to the fetus, fuse and come in contact with maternal blood, thus facilitating nutrient and waste exchange between the mother and the baby. Abnormalities of the placenta may occur as a result of cellular stress and have been associated with pregnancy-associated disorders: such as preeclampsia, intrauterine growth restriction, and placental insufficiency. Cellular stress has also been shown to alter proliferation and differentiation rates of trophoblast cells. This stress response is important for cell survival and ensures continued placental functionality. AMP-activated protein kinase is an important sensor of cellular metabolism and stress. To study the role of AMPK in the trophoblast cells, we used RNA interference to simultaneously knockdown levels of both the AMPK alpha isoforms, AMPKa1 and AMPKa2. SM10 trophoblast progenitor cells were transduced with AMPKa1/2 shRNA and stable clones were established to analyze the effects of AMPK knockdown on important cellular functions. Our results indicate that a reduction in AMPK levels causes alterations in cell morphology, growth rate, and nutrient transport, thus identifying an important role for AMPK in the regulation of placental trophoblast differentiation.

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“…Fetal consumption of glucose rapidly increases toward term due to the almost 20-fold increase in fetal weight during the second half of gestation [18]. Since fetuses are not able to generate glucose de novo until late in gestation, fetal glucose usage is critically dependent upon the transfer system across the placenta [4,21].…”

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confidence: 99%

Quantitative Expression and Immunohistochemical Detection of Glucose Transporters, GLUT1 and GLUT3 in the Rabbit Placenta during Successful Pregnancy

Khan

Kusakabe

Wakitani

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. Glucose is essential for the development of the fetus. We address here the quantitative expression and immunohistochemical localization of glucose transporter (GLUT1 and GLUT3) in the rabbit placenta during successful pregnancy. Blood glucose level showed a significant decrease at the gestation period in comparison with non-pregnancy. Maternal serum glucose was gradually increased according to fetal development. Quantitative RT-PCR results showed that expression of GLUT1 was significantly increased from day 13 to day 18, while GLUT3 mRNA level was significantly decreased during the same periods. Western blot analysis demonstrated that GLUT1 protein did not change significantly in the placenta during pregnancy when compared to non-pregnant uteri. Immunohistochemistry indicated that distribution of GLUT1 was observed mainly to the surface of the outer trophoblasts, whereas GLUT3 mainly localized to the basal site of the inner trophoblasts and fetal blood vessels. These results suggest that glucose is transported through GLUT1 from the maternal blood stream for use as a placental fuel and for further transport through GLUT3 to the fetal circulation, thus signifying the distinct anatomical localization of GLUT1 and GLUT3 in the rabbit placenta during successful pregnancy.KEY WORDS: GLUT1, GLUT3, immunohistochemistry, mRNA, rabbit placenta.

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Glucose transporter isoform 4 is expressed in the syncytiotrophoblast of first trimester human placenta

Abstract: The insulin-regulatable GLUT4 is expressed in the cytosol of first trimester ST compatible with a role for GLUT4 in placental glucose transport in early pregnancy. The placental expression pattern of GLUT isoforms in early pregnancy is distinct from that later in pregnancy.

Cited by 115 publications

References 44 publications

Id2 Mediates Differentiation of Labyrinthine Placental Progenitor Cell Line, SM10

Id2 Mediates Differentiation of Labyrinthine Placental Progenitor Cell Line, SM10

AMPK Knockdown in Placental Trophoblast Cells Results in Altered Morphology and Function

Quantitative Expression and Immunohistochemical Detection of Glucose Transporters, GLUT1 and GLUT3 in the Rabbit Placenta during Successful Pregnancy

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