Glucose Uptake by the Isolated Perfused Rat Hind Limb during Rest and Exercise

Aj, Szabo; Rj, Mahler; Szabó, Olga

doi:10.1055/s-0028-1095147

Cited by 18 publications

(8 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Stimulation caused a tenfold increase in the rate of lactate production and a twoto four-fold increase in 02 consumption. In the presence of excess of insulin (25munits/ml) electrical stimulation caused no extra glucose uptake whereas in the absence of insulin, stimulation caused an increase in glucose uptake from 0.75 to 4.22,umol/min per 30g of muscle, in agreement with similar findings by Szabo et al (1969). The increased glucose uptake could account for no more than 35% of the increased lactate production.…”

Section: Resultssupporting

confidence: 89%

“…Experiments on investigation of the metabolic performance of the the hindquarter of the rat have been reported by isolated perfused hindquarter of the rat, a preseveral investigators (Robinson & Harris, 1959; paration consisting mainly of muscle tissue. It Forsander, Raiha & Suomalainen, 1960;Mortimore, was prompted by the experience that isolated Tietze & Stetten, 1959;Mahler, Szabo & Penhos, p3rfused organs are very useful for the study of 1968; Szabo, Mahler & Szabo, 1969), but it appears control mechanisms of metabolism. Hindquarters that no systematic effort has been made to compare of larger animals such as dogs and cats have often the activities of the isolated preparation with those been used in the past (Burn & Dale, 1926; Best, of the hindquarter in 8itu.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of the isolated perfused rat hindquarter for the study of muscle metabolism

Ruderman

Houghton

Hems

1971

Biochemical Journal

443

226

View full text Add to dashboard Cite

1. The metabolic integrity of a new isolated rat hindquarter preparation was studied. The hindquarter was perfused with a semi-synthetic medium containing aged human erythrocytes. More than 95% of the oxidative metabolism of the preparation was due to muscle, the remainder being due to bone, adipose tissue and, where present, skin. 2. Consumption of O(2), glucose utilization, glycerol release and lactate production were similar in the presence and in the absence of the skin, indicating that the latter contributed little to the overall metabolism of the preparation. 3. After 40min of perfusion, tissue concentrations of creatine phosphate, ATP and ADP were similar to those found in muscle taken directly from intact animals. The muscle also appeared normal under the electron microscope. 4. The hindquarter did not lose K(+) to the medium during a 30min perfusion. In the presence of insulin it had a net K(+) uptake. 5. Insulin caused a sixfold increase in glucose uptake, stimulated O(2) consumption by nearly 40% and depressed glycerol release to less than half the control value. 6. Bilateral sciatic-nerve stimulation caused severalfold increases in O(2) consumption and lactate production. In the absence of insulin nerve stimulation also enhanced glucose uptake; in the presence of insulin it did not further increase the already high rate of glucose uptake. 7. Rates of lactate production and O(2) consumption of the rat hindquarter in vivo and the isolated perfused hindquarter were very similar. 8. Ketone bodies were a major oxidative fuel in vivo of the hindquarter of a rat starved for 2 days. If the acetoacetate and 3-hydroxybutyrate removed by the tissue were completely oxidized, they would have accounted for 77% of the O(2) consumption. 9. Acetoacetate accounted for 84% of the ketone bodies removed by the hindquarter in vivo even though its arterial concentration was half that of 3-hydroxybutyrate. 10. Similar rates of acetoacetate and 3-hydroxybutyrate utilization were observed in the perfused hindquarter. 11. Acetoacetate utilization by the perfused hindquarter was not diminished by the addition of either oleate or insulin to the perfusate. 12. Oxidation of glucose to CO(2) accounted for less than 4% of the O(2) consumed by the perfused hindquarter in both the presence and the absence of insulin. 13. The results indicate that the isolated perfused hindquarter is a useful tool for studying muscle metabolism. They also suggest that ketone bodies, if present in sufficient concentration, are the preferred oxidative fuel of resting muscle.

show abstract

Section: Resultssupporting

confidence: 89%

mentioning

confidence: 99%

Evaluation of the isolated perfused rat hindquarter for the study of muscle metabolism

Ruderman

Houghton

Hems

1971

Biochemical Journal

443

226

View full text Add to dashboard Cite

show abstract

“…In fed and starved rats, exercise caused an 8-12-fold increase in glucose uptake when insulin was not added to the perfusate (Table 3). This is in accordance with earlier work carried out with exercising hindlimb and hindquarter preparations (McGuigan, 1908;Huycke & Kruhoffer, 1955;Szabo et al, 1969;Ruderman et al, 1971). In the presence of added insulin, exercise caused a further increase in the uptake of glucose from 5.2±0.4 to 8.4±0.6,umol/min per 30g of muscle in fed rats and from 5.4±0.7 to 8.8±0.6,umol/min per 30g of muscle in starved rats.…”

Section: Glucose Uptake By Exercising Musclesupporting

confidence: 93%

Glucose metabolism in perfused skeletal muscle. Interaction of insulin and exercise on glucose uptake

Berger

Hagg

Ruderman

1975

Biochemical Journal

206

View full text Add to dashboard Cite

1. The interaction of insulin and isometric exercise on glucose uptake by skeletal muscle was studied in the isolated perfused rat hindquarter. 2. Insulin, 10 m-i.u./ml, added to the perfusate, increased glucose uptake more than 10-fold, from 0.3-0.5 to 5.2-5.4 mumol/min per 30g of muscle in hindquarters of fed and 48h-starved rats respectively. In contrast, it did not stimulate glucose uptake in hindquarters from rats in diabetic ketoacidosis. 3. In the absence of added insulin, isometric exercise, induced by sciatic-nerve stimulation, increased glucose uptake to 4 and 3.4 mumol/min per 30g of muscle in fed and starved rats respectively. It had a similar effect in rats with moderately severe diabetes, but it did not increase glucose uptake in rats with diabetic ketoacidosis or in hindquarters of fed rats that had been "washed out" with an insulin-free perfusate. Insulin, at concentrations which did not stimulate glucose uptake in resting muscle, restored the stimulatory effect of exercise in these situations. 4. The stimulation of glucose uptake by exercise was independent of blood flow and the degree of tissue hypoxia; also it could not be reproduced by perfusing resting muscle with a medium previously used in an exercise experiment. 5. At rest glucose was not detectable in muscle cell water of fed and starved rats even when perfused with insulin. In the presence of insulin, a small accumulation of glucose, 0.25 mM, was noted in the muscle of ketoacidotic diabetic rats, suggesting inhibition of glucose phosphorylation, as well as of transport. 6. During exercise, the calculated intracellular concentration of glucose in the contracting muscle increased to 1.1-1.6mM in the fed, starved and moderately diabetic groups. Insulin significantly increased the already high rates of glucose uptake by the hindquarters of these animals but it did not alter the elevated intracellular concentration of glucose. 7. In severely diabetic rats, exercise did not cause glucose to accumulate in the cell in the absence of insulin. In the presence of insulin, it increased glucose uptake to 6.1 mumol/min per 30g of muscle and intracellular glucose to 0.72 mM. 8. The data indicate that the stimulatory effect of exercise on glucose uptake requires the presence of insulin. They suggest that in the absence of insulin, glucose uptake is not enhanced by exercise owing to inhibition of glucose transport into the cell.

show abstract

“…The change in glucose concentration during perfusion is shown in table 2. Even though glucose was continuously infused in all treatments (28 mg.kg hind limb-X.h-1), glucose levels decreased after 17 min of perfusion (this initial lag has been observed by other researchers; Szabo et al, 1969), and is likely due to initial utilization (or depletion) of muscle glycogen reserves. Increased muscular stimulation caused greater decreases in glucose concentration at the 32-min sampling time and thereafter, because of the increased energy muscle metabolism was increased during needs of the exercised muscle.…”

Section: Resultssupporting

confidence: 55%

Isolation-Perfusion of Ovine Hind Limbs. IV. Effects of Level of Activity on Amino Acid and Glucose Metabolism

Ww¹,

Lc²,

Ja³

et al. 1984

Journal of Animal Science

View full text Add to dashboard Cite

Twenty-one wether lambs averaging 30.0 kg were utilized in a study to determine the influence of level of muscular activity (via electrical stimulation) on metabolism of amino acids and glucose of isolated-perfused hind limbs. Treatments consisted of: (1) control, (2) low electrical stimulation and (3) high electrical stimulation. Mean perfusion flow rate was unaffected by treatment or time of perfusion. Perfusion pressure and hematocrit were increased (P less than .05) by stimulation. Glucose levels decreased from a pretreatment average of 44.7 to 41.3, 26.8 and 20.0 mg/dl for treatments 1 to 3, respectively. Lactic acid levels increased from the pretreatment mean of 37.7 to 46.7, 63.8 and 69.4 mg/dl, respectively. Plasma-free fatty acids were utilized from the perfusate at .84, .76 and .68 meq X liter-1 X min-1, respectively. Perfusate urea N levels were unaffected by treatment or time of sampling, but perfusate ammonia levels increased in all treatments (ammonia levels for treatments 1 to 3 increased by .50, 1.09 and 1.47 mg/dl, respectively). Perfusate amino acid changes suggested a flux of some amino acids from muscle to perfusate due to electrical stimulation, but perfusate branched chain amino acid concentrations decreased in all treatment groups. Fifty microcuries of 14C-lysine were included in the initial perfusate. Total perfusate radioactivity in all treatments declined with time, reflective of lysine uptake by the muscle. Total perfusate lysine concentration changed less markedly, suggesting that muscle is contributing to maintenance of plasma amino acid levels.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

Glucose Uptake by the Isolated Perfused Rat Hind Limb during Rest and Exercise

Cited by 18 publications

References 5 publications

Evaluation of the isolated perfused rat hindquarter for the study of muscle metabolism

Evaluation of the isolated perfused rat hindquarter for the study of muscle metabolism

Glucose metabolism in perfused skeletal muscle. Interaction of insulin and exercise on glucose uptake

Isolation-Perfusion of Ovine Hind Limbs. IV. Effects of Level of Activity on Amino Acid and Glucose Metabolism

Contact Info

Product

Resources

About