Glucose utilization by edematous rat lungs

Young, Stephen L.; O’Neil, John J.; Kasuyama, R. S.; Tierney, Donald F.

doi:10.1007/bf02713613

Cited by 11 publications

(4 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our report that sodium-potassium-ATPase was significantly reduced one day postexposure to 1.0 ppm phosgene is consistent with the elevation of ATP per lung above control values on days 2 and 3 postexposure to phosgene (11). The report by Young et a1 that a doubling of glycolysis occurs during edema may account for some of the elevatio of ATP above control (20). Following pulmonary edema generated by medh t nical or hydrostatic stress, Postlethwait and Young reported a 20% decrease in ATP concentration per milligram DNA (22).…”

Section: Discussionsupporting

confidence: 89%

“…The mitochondrial effect is reversible, consistent with the observed lowered ATP values that returned to normal levels after exposure to phosgene. Young et a1 have reported that the oxygen consumption in tissue slices from lungs made edematous by raising left atrial pressure could not be stimulated as much as controls; therefore, a functional mitochondrial abnormality may be caused by edema (20). Frosolono and Pawlowski have shown that phosgene exposures caused a significant decrease in cytochrome c oxidase activity in rat lung (6).…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Changes in lung ATP concentration in the rat after low‐level phosgene exposure

Currie

Hatch

Frosolono

1987

J. Biochem. Toxicol.

View full text Add to dashboard Cite

Inhibition of mitochondrial respiratory activity and decreased lung adenosine triphosphate (ATP) concentration occur following exposure to 240 ppm.min phosgene. To determine the relationship between energy stores and the onset of phosgene-induced pulmonary edema, we measured the ATP concentration in rapidly frozen rat lung tissue before and during pulmonary edema. Male Sprague-Dawley rats were exposed to phosgene for four hours at concentrations of 0.05 to 1.0 ppm (12, 30, 60, 120, and 240 ppm.min). Lung wet and dry weight and ATP concentration were measured immediately after exposure and for three days postexposure. The accumulation of lavage fluid protein (LFP) was also measured as an index of damage or edema due to phosgene. Lung dry weight was significantly elevated one day postexposure to 0.5 ppm phosgene, while the LFP was elevated by 0.2 ppm phosgene. Time course studies at these doses of phosgene showed that decreased ATP levels preceded the onset of edema or increase in lung weight. The ATP values expressed on a per-lung basis showed that ATP levels were significantly lowered immediately following phosgene exposure, suggesting that the ATP changes were not the result of edema. This study is the first demonstration of a biochemical change that occurs following exposure to phosgene at a level significantly below the threshold limit value for this gas.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 97%

Changes in lung ATP concentration in the rat after low‐level phosgene exposure

Currie

Hatch

Frosolono

1987

J. Biochem. Toxicol.

View full text Add to dashboard Cite

show abstract

“…[37][38][39] One striking finding is that for an organ with one of the highest oxygen tensions in the body, the lung produces considerable quantities of lactate. 24 Indeed, 40% to 60% of the glucose used by the lungs ends up as lactate.…”

Section: Lung Metabolism During Perfusionmentioning

confidence: 98%

State of Art: Clinical ex vivo lung perfusion: Rationale, current status, and future directions

Sánchez¹,

Bittle²,

Burdorf³

et al. 2012

The Journal of Heart and Lung Transplantation

View full text Add to dashboard Cite

“…Carbohydrate oxidation has also been studied in lung tissue slices. The relative activities of glycolysis and tricarboxylic acid metabolism versus the pentose phosphate shunt have been described by various investigators in both normal (1) and injured lung tissue (33). These experiments often involve the measurement of '4CO2 evolved from glucose labeled in the C-6 and C-1 positions and the quantitation of intermediates such as lactate.…”

Section: Intermediary Metabolism In Lung Tissue Slicesmentioning

confidence: 99%

Tissue slices in the study of lung metabolism and toxicology.

Freeman¹,

O’Neil²

1984

Environ Health Perspect

View full text Add to dashboard Cite

Lung tissue slices are model systems for the study of pulmonary metabolism. Because of the speed and simplicity of slice preparation, lung slices have been used in studies of oxygen, amino acid, carbohydrate and lipid utilization and adenine nucleotide metabolism. Dose-response characteristics for toxicants are readily described because multiple lung samples can be studied from the same animal or a population of animals. Lung slices prepared from animals exposed to oxidant air pollutants exhibit alterations in respiration, glucose consumption and lipid metabolism. These studies have indicated both direct toxic effects of air pollutants on enzyme systems and also air pollutant-induced changes in the cellularity of lungs.

show abstract

Glucose utilization by edematous rat lungs

Cited by 11 publications

References 14 publications

Changes in lung ATP concentration in the rat after low‐level phosgene exposure

Changes in lung ATP concentration in the rat after low‐level phosgene exposure

State of Art: Clinical ex vivo lung perfusion: Rationale, current status, and future directions

Tissue slices in the study of lung metabolism and toxicology.

Contact Info

Product

Resources

About