Glucosinolates and phenolic compounds rich broccoli extract: Encapsulation by electrospraying and antitumor activity against glial tumor cells

Radünz, Marjana; Hackbart, Helen Cristina dos Santos; Bona, Natália Pontes; Pedra, Nathália Stark; Hoffmann, Jéssica Fernanda; Stefanello, Francieli Moro; Zavareze, Elessandra da Rosa

doi:10.1016/j.colsurfb.2020.111020

Cited by 42 publications

(37 citation statements)

References 36 publications

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“…Nowadays, there is increasing consumer concern about the nutritional quality of food, as vegetable origin foods are key for a healthy diet. The role of these plant-based foods in the prevention of a wide array of chronic diseases is associated with their content in bioactive compounds (nutrients and non-nutrients), and to the modification of molecular pathways responsible for the onset and advance of pathophysiological processes [1,2]. Regarding this point, cruciferous sprouts have been demonstrated to be a source of valuable bioactive micronutrients (vitamins and minerals) and phytochemical compounds (glucosinolates and phenolics), related to the prevention of inflammatory, tumoral, or metabolic disorders [3,4].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Evidence on Bioaccessibility of Flavonols and Cinnamoyl Derivatives of Cruciferous Sprouts

et al. 2021

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Cruciferous sprouts are rising in popularity as a hallmark of healthy diets, partially because of their phytochemical composition, characterized by the presence of flavonols and cinnamates. However, to shed light on their biological activity, the ability to assimilate (poly)phenols from sprouts (bioaccessible fraction) during gastrointestinal digestion needs to be studied. In this frame, the present work studies the effect of the physicochemical and enzymatic characteristics of gastrointestinal digestion on flavonols and cinnamoyl derivatives, by a simulated static in vitro model, on different cruciferous (red radish, red cabbage, broccoli, and white mustard) sprouts. The results indicate that, although the initial concentrations of phenolic acids in red radish (64.25 mg/g fresh weight (fw)) are lower than in the other sprouts studied, their bioaccessibility after digestion is higher (90.40 mg/g fw), followed by red cabbage (72.52 mg/g fw), white mustard (58.72 mg/g fw), and broccoli (35.59 mg/g fw). These results indicate that the bioaccessibility of (poly)phenols is not exclusively associated with the initial concentration in the raw material, but that the physico-chemical properties of the food matrix, the presence of other additional molecules, and the specific characteristics of digestion are relevant factors in their assimilation.

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Section: Introductionmentioning

confidence: 99%

In Vitro Evidence on Bioaccessibility of Flavonols and Cinnamoyl Derivatives of Cruciferous Sprouts

et al. 2021

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“…Gliomas are the most common primary craniocerebral malignancies caused by cancerous changes in glial cells (26). The incidence rate of glioma among intracranial tumors is 35.2-61.0% (1).…”

Section: Discussionmentioning

confidence: 99%

cRGDyK‑modified procaine liposome inhibits the proliferation and motility of glioma cells via the ERK/p38MAPK pathway

Gao

Yang

et al. 2021

Exp Ther Med

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Glioma is a common type of primary tumor in the central nervous system. Glioma has been increasing in incidence yearly and is a serious threat to human life and health. The aim of the present study was to prepare liposomes for enhanced penetration of the blood-brain barrier and targeting of glioma. A procaine-loaded liposome modified with the cyclic pentapeptide cRGDyK (Pro/cRGDyK-L) was designed and developed. The particle size, ζ potential, encapsulation efficiency, release profile, stability and hemolysis of Pro/cRGDyK-L were characterized in vitro. The targeting and antitumor effects of Pro/cRGDyK-L were also investigated in vitro and in vivo. The results suggested that the cRGDyK peptide significantly facilitated the ability of liposomes to transfer procaine across the BBB and improved the cellular uptake of procaine by C6 glioma cells. The results further demonstrated that Pro/cRGDyK-L strongly suppressed cell motility, stimulated apoptosis and induced cell cycle arrest. The findings further confirmed that Pro/cRGDyK-L exhibited superior antitumor effects by targeting the ERK/p38MAPK pathway and thereby suppressed tumor growth in mice. In conclusion, the present study indicated the potential of Pro/cRGDyK-L as a means to provide improved therapeutic effects on glioma through the ERK/p38MAPK pathway.

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“…Presently, a global trend has been observed on the demand for highly nutritious and easy-to-consume-foods, including functional foods or beverages [ 18 ]. Different sources of ingredients from Brassica species have been used to date [ 14 , 19 ], which are usually prepared as extracts rich in ITCs [ 20 , 21 ]. The problems with using ITCs as ingredients include their low water solubility and very limited stability [ 22 , 23 ], when used in nutritional interventions and compared to the intake of GSLs from a natural matrix (e.g., broccoli sprouts) [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

The Influence of Red Cabbage Extract Nanoencapsulated with Brassica Plasma Membrane Vesicles on the Gut Microbiome of Obese Volunteers

García-Ibáñez

Rosés

Agudelo

et al. 2021

Foods

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The aim of the study was to evaluate the influence of the red cabbage extracts on the bioaccessibility of their isothiocyanates, and their effect on the intestinal microbiota using a dynamic model of human digestion treated with the gut microbiome of obese adults. The elicitation of red cabbage plants with methyl jasmonate (MeJA) duplicated the content of glucosinolates (GSLs) in the plant organs used for elaborating the encapsulated formula. The use of plasma membrane vesicles, according to a proper methodology and technology, showed a high retention of sulforaphane (SFN) and indol-3-carbinol (I3C) over the course of the 14-day digestion study. The microbiome was scarcely affected by the treatments in terms of microbiota composition or the Bacteroidetes/Firmicutes ratio, but a 3 to 4-fold increase was observed in the production of butyric acid with the encapsulated extract treatment. Based on our pilot red cabbage extract study, the consumption of this extract, mainly encapsulated, may play a potential role in the management of obesity in adults.

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Glucosinolates and phenolic compounds rich broccoli extract: Encapsulation by electrospraying and antitumor activity against glial tumor cells

Cited by 42 publications

References 36 publications

In Vitro Evidence on Bioaccessibility of Flavonols and Cinnamoyl Derivatives of Cruciferous Sprouts

In Vitro Evidence on Bioaccessibility of Flavonols and Cinnamoyl Derivatives of Cruciferous Sprouts

cRGDyK‑modified procaine liposome inhibits the proliferation and motility of glioma cells via the ERK/p38MAPK pathway

The Influence of Red Cabbage Extract Nanoencapsulated with Brassica Plasma Membrane Vesicles on the Gut Microbiome of Obese Volunteers

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