Glucosinolates profile, volatile constituents, antimicrobial, and cytotoxic activities of Lobularia libyca

Abstract

Context: Brassicaceae plants are associated with protection against cancers due to their glucosinolate contents. Objectives: We investigate fresh leaves, roots and ripe seeds of Lobularia libyca (Viv.) C.F.W. Meissn. (Brassicaceae) to identify their glucosinolate constituents, antimicrobial and cytotoxic activities Materials and methods: The glucosinolates were identified using GC-MS analysis of their hydrolysis products and LC-MS analysis in the case of seeds. Disc diffusion (1 mg/disc) and minimum inhibitory concentration (0-160 lg/mL) methods were used to evaluate the antimicrobial activity of seed hydrolysate. In vitro cytotoxicity against colorectal HCT-116, hepatic HUH-7, breast MCF-7 and lung A-549 cells was evaluated for seed hydrolysate (0.01-100 lg/mL) using the sulforhodamine B assay and doxorubicin as a standard Results: Three glucosinolates were identified for the first time in this plant and genus Lobularia. Glucoiberverin was the major compound accumulated in the seeds and leaves, while glucoiberin and glucoerucin were detected only in the seeds. No glucosinolates were detected in roots under the same experimental conditions. Other volatile constituents, e.g., terpenes and fatty acids were only identified in the seeds. The seed hydrolysate showed significant antimicrobial activities against Candida albicans and Pseudomonas aeruoginosa (MIC ¼ 64 and 82 lg/mL, respectively). The seed hydrolysate exhibited a marked selective cytotoxicity in vitro against colorectal, hepatic and breast cancer cell lines. The IC 50 values were 0.31, 2.25 and 37 lg/mL, respectively. Discussion and conclusion: The results indicated the antimicrobial activity of L. libyca and the selective effect of the seed hydrolysate as a cytotoxic drug that is potentially more active than doxorubicin against HCT-116. ARTICLE HISTORY

Antimicrobial Resistance and the Alternative Resources with Special Emphasis on Plant-Based Antimicrobials—A Review

“…aeruginosa . It was shown that seed hydrolysates have significant activity against both tested microorganisms [46]. …”

Microwave-Assisted versus Conventional Isolation of Glucosinolate Degradation Products from Lunaria annua L. and Their Cytotoxic Activity

“…The calculation of IC 50 values was performed using GraphPad Prism software version 7.0. The criteria used to categorize the activity against the tested cell lines was based on IC 50 values as follows: 20 µg/mL = highly active, 21 to 200 µg/mL = moderately active, 201 to 500 µg/mL = weakly active, and >501 µg/mL = inactive [20].…”

Role of Some Wild Herbal Extracts in Management of American Foulbrood in Honeybee Colonies

“…Lobularia arabica methanolic extract was the least effective in AFB control and on the population parameters of the Carniolan honey bees among the three herbal extracts. Al-Gendy et al (2016) identified three glucosinolates from the genus Lobularia lybica with antimicrobial and cytotoxic activities.…”