1991
DOI: 10.1104/pp.95.1.152
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Glucosylation of Steviol and Steviol-Glucosides in Extracts from Stevia rebaudiana Bertoni

Abstract: To evaluate and characterize stevioside biosynthetic pathway in Stevia rebaudiana Bertoni cv Houten, two enzyme fractions that catalyze glucosylation of steviol (ent-13-hydroxy kaur-16-en-19-oic acid) and steviol-glucosides (steviol-13-0-glucopyranoside, steviolbioside and stevioside), utilizing UDP-glucose as the glucose donor, were prepared from the soluble extracts of S. rebaudiana leaves. Enzyme fraction 1, passed through DEAE-Toyopearl equilibrated with 50 millimolar K-phosphate pH 7.5, catalyzed the gluc… Show more

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Cited by 47 publications
(36 citation statements)
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“…These correlations can be partially explained by the biosynthetic relationships between the individual glycosides. For example, stevioside is the substrate for the synthesis of rebaudioside A, plants high in rebaudioside A will probably be low in stevioside (Shibata et al 1991).…”
Section: Cultivar Developmentmentioning
confidence: 99%
See 1 more Smart Citation
“…These correlations can be partially explained by the biosynthetic relationships between the individual glycosides. For example, stevioside is the substrate for the synthesis of rebaudioside A, plants high in rebaudioside A will probably be low in stevioside (Shibata et al 1991).…”
Section: Cultivar Developmentmentioning
confidence: 99%
“…1) sequence of glycosylations that gives rise to the different glycan side chains is still in the early stages of elucidation. At least three distinct glycosyltransferase activities have been identified (Shibata et al 1991(Shibata et al , 1995. Two of these activities have been purified and characterized.…”
Section: The Glycoside Side Chainsmentioning
confidence: 99%
“…Different SGs are formed by glucosylation of steviol by specific glucosyltransferases (GTs) (Shibata et al, 1991(Shibata et al, , 1995.…”
Section: Introductionmentioning
confidence: 99%
“…As Czech J. Food Sci., 34, 2016 (4): 281-299 doi: 10.17221/335/2015-CJFS a final point, glycosylation of steviolbioside occurs at C-19 position by UGT74G1 to form stevioside (Shibata 1991). Rebaudioside A is synthesised by glucosylation at C-13 of stevioside catalysed by the enzyme UGT76G1 (Brandle & Telmer 2007).…”
Section: Biosynthetic Pathwaymentioning
confidence: 99%