Glut1 promotes cell proliferation, migration and invasion by regulating epidermal growth factor receptor and integrin signaling in triple-negative breast cancer cells

Abstract

Elevated glucose levels in cancer cells can be attributed to increased levels of glucose transporter (GLUT) proteins. Glut1 expression is increased in human malignant cells. To investigate alternative roles of Glut1 in breast cancer, we silenced Glut1 in triple-negative breast-cancer cell lines using a short hairpin RNA (shRNA) system. Glut1 silencing was verified by Western blotting and qRT-PCR. Knockdown of Glut1 resulted in decreased cell proliferation, glucose uptake, migration, and invasion through modulation of the EGFR/MAPK signaling pathway and integrin β1/Src/FAK signaling pathways. These results suggest that Glut1 not only plays a role as a glucose transporter, but also acts as a regulator of signaling cascades in the tumorigenesis of breast cancer.

Lipocalin 2 negatively regulates cell proliferation and epithelial to mesenchymal transition through changing metabolic gene expression in colorectal cancer

“…GLUT, initiators of glycolytic pathways, are highly likely to be involved in the EMT process. In particular, GLUT1 shows a strong association with invasive ability and contributes to tumor aggressiveness . GLUT3 expression also influences the EMT process, because enhanced expression of GLUT3 is induced by ectopic expression of Zinc finger E‐box binding homeobox 1 (ZEB1) or Snail in non‐small lung cancer.…”

“…In particular, GLUT1 shows a strong association with invasive ability and contributes to tumor aggressiveness. (39,40) GLUT3 expression also influences the EMT process, because enhanced expression of GLUT3 is induced by ectopic expression of Zinc finger E-box binding homeobox 1 (ZEB1) or Snail in non-small lung cancer. At the same time, some EMT markers have been reported to be glycolytic switches for energy metabolism.…”

E‐cadherin expression increases cell proliferation by regulating energy metabolism through nuclear factor‐κB in AGS cells

“…Malignant cancer cells tend to show a higher rate of glycolysis and glucose absorption; these processes are controlled by glucose transport proteins, such as GLUT. (41) Glucose transporter 1 is overexpressed in many malignant tumors (42,43) and a high level of GLUT1 expression was suggested as a predictor of a poor prognosis. (44) The mRNA levels of GLUT1, GLUT3, and GLUT4 increased, and the increase in that of GLUT1 was most pronounced (Fig.…”

Topical administration of hydroethanolic extract of Lawsonia inermis (henna) accelerates excisional wound healing process by reducing tissue inflammation and amplifying glucose uptake

“…The glucose transporter proteins are known to be the key structures in transferring glucose into mammalian cells. Glucose transporter‐1 (Glut‐1) is not only responsible for basal glucose uptake but also encourages cell proliferation and migration by regulating epidermal growth factor receptor in cancer cells . Insulin‐like growth factor I (Igf‐1) is not only transported glucose into cell during wound healing but also modulated cell proliferation and migration during the fibroplasia stage of wound healing in rats .…”

“…Glucose transporter-1 (Glut-1) is not only responsible for basal glucose uptake but also encourages cell proliferation and migration by regulating epidermal growth factor receptor in cancer cells. 5 Insulin-like growth factor I (Igf-1) is not only transported glucose into cell during wound healing but also modulated cell proliferation and migration during the fibroplasia stage of wound healing in rats. 6 It has also been known to be a key factor in myofibroblast differentiation and proliferation because of its mitogenic activity.…”