Glutamate decarboxylase activity and gamma-aminobutyric acid content in Synechocystis sp. PCC 6803 under osmotic stress and different carbon sources

Kanwal, Simab; Rastogi, Rajesh P.; Incharoensakdi, Aran

doi:10.1007/s10811-014-0259-9

Cited by 27 publications

(11 citation statements)

References 36 publications

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“…At lower levels of NaCl (1-3%), it seemed to be able to enhance GABA production. Activation of glutamate decarboxylase (GAD) responsible for the synthesis of GABA could be observed in the presence of osmotic stress by NaCl or D-sorbitol (Kanwal et al, 2014). This may also be correlated with the source of isolation since salt content added in Plaa-som regularly ranges from approximately 6-11% (Paludan-Müller et al, 2002).…”

Section: Effect Of Culture Conditions For Gaba Products In Mrs Mediummentioning

confidence: 99%

Screening and characterisation of gamma-aminobutyric acid (GABA) producing lactic acid bacteria isolated from Thai fermented fish (Plaa-som) in Nong Khai and its application in Thai fermented vegetables (Som-pak)

2020

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This study aimed to evaluate the capability of lactic acid bacteria (LAB) isolated from different kinds of fermented fish products (Plaa-som) on the production of γ-aminobutyric acid (GABA). Among them, isolate L10-11, identified as Lactobacillus plantarum, offered the highest GABA production and was selected for further study. The highest production of GABA was obtained within 48h from the de Man, Rogosa and Sharpe (MRS) medium, having a pH range of 5-6. Oncreasing the monosodium glutamate (MSG) concentration resulted in a higher accumulation of GABA and reached the highest concentration (15.74 g/L) using 4% (w/v) MSG, while high residual MSG was also observed in accordance with concentration increase. Dn the other hand, the addition of NaCl in the culture medium by up to 7% (w/v) did not suppress GABA production. Preliminary application of strain L10-11 as starter producing GABA was investigated in Thai fermented vegetables (Som-pak). Ot was found that GABA formation could be observed increasingly following the concentration of MSG added. GABA content in Som-pak was 5 times higher than that of control when inoculating Lb. plantarum L10-11 and adding MSG at 1% (w/v). On addition, sensory evaluation revealed that addition of this starter culture to Som-pak gave overall acceptability slightly higher than that which allowed fermentation to occur spontaneously. This alternative procedure would be successful for improving the nutritional quality of functional fermented food.

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Section: Effect Of Culture Conditions For Gaba Products In Mrs Mediummentioning

confidence: 99%

Screening and characterisation of gamma-aminobutyric acid (GABA) producing lactic acid bacteria isolated from Thai fermented fish (Plaa-som) in Nong Khai and its application in Thai fermented vegetables (Som-pak)

2020

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“…In C. vulgaris strain UAM 101, the apparent affinity of CO 2 was reduced during CO 2 fixation in the presence of glucose [ 51 ]. Moreover, glucose is known to induce osmotic stress in Synechocystis 6803 [ 52 ]; stress conditions might cause a reduction in photosynthetic efficiency of algae cells. Reduction of photosynthetic activity during early exposure to glucose might be caused by limited photosynthetic gene expression, reduced CO 2 affinity , or increased osmotic stress.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Production of Photosynthetic Pigments and Various Metabolites and Lipids in the Cyanobacteria Synechocystis sp. PCC 7338 Culture in the Presence of Exogenous Glucose

et al. 2021

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Synechocystis strains are cyanobacteria that can produce useful biomaterials for biofuel and pharmaceutical resources. In this study, the effects of exogenous glucose (5-mM) on cell growth, photosynthetic pigments, metabolites, and lipids in Synechocystis sp. PCC 7338 (referred to as Synechocystis 7338) were investigated. Exogenous glucose increased cell growth on days 9 and 18. The highest production (mg/L) of chlorophyll a (34.66), phycocyanin (84.94), allophycocyanin (34.28), and phycoerythrin (6.90) was observed on day 18 in Synechocystis 7338 culture under 5-mM glucose. Alterations in metabolic and lipidomic profiles under 5-mM glucose were investigated using gas chromatography-mass spectrometry (MS) and nanoelectrospray ionization-MS. The highest production (relative intensity/L) of aspartic acid, glutamic acid, glycerol-3-phosphate, linolenic acid, monogalactosyldiacylglycerol (MGDG) 16:0/18:1, MGDG 16:0/20:2, MGDG 18:1/18:2, neophytadiene, oleic acid, phosphatidylglycerol (PG) 16:0/16:0, and PG 16:0/17:2 was achieved on day 9. The highest production of pyroglutamic acid and sucrose was observed on day 18. We suggest that the addition of exogenous glucose to Synechocystis 7338 culture could be an efficient strategy for improving growth of cells and production of photosynthetic pigments, metabolites, and intact lipid species for industrial applications.

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“…The supernatant was collected and dried in a vacuum evaporator at 40°C. Pellet left after drying was dissolved and mixed thoroughly in 250 μL of water and 50 μL of chloroform followed by centrifugation at 6,000 × g for 10 min (Kanwal et al, 2014). The uppermost water phase (200 μL) was collected, and filtered through a 0.45 μm Millipore filter before the analysis of acetate, ethanol, pyruvate, and succinate by HPLC.…”

Section: Methodsmentioning

confidence: 99%

Metal Oxide Mediated Extracellular NADPH Regeneration Improves Ethanol Production by Engineered Synechocystis sp. PCC 6803

Velmurugan

Incharoensakdi

2019

Front. Bioeng. Biotechnol.

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The ethanol synthesis pathway engineered Synechocystis sp. PCC 6803 (hereafter Synechocystis ) was used to investigate the influence of metal oxide mediated extracellular NADPH regeneration on ethanol synthesis. The in-vitro studies proved that the metal oxides have the potential to generate the NADPH in the presence of electron donor, the usual components of photoautotrophic growth conditions. When the NADPH regeneration was applied in Synechocystsis , the strain showed improved growth and ethanol production. This improved ethanol synthesis is attributed to the increased availability of NADPH to the ethanol synthesis pathway and redirection of closely related carbon metabolism into the ethanol synthesis. Under optimized light intensity and NADP addition, the maximum ethanol production of 5,100 mg/L was observed in MgO mediated extracellular NADPH regeneration after 25 days of cultivation, which is 2-fold higher than the control. This study indicates the feasibility of metal oxide mediated extracellular NADPH regeneration of Synechocystis to increase the production of ethanol.

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Glutamate decarboxylase activity and gamma-aminobutyric acid content in Synechocystis sp. PCC 6803 under osmotic stress and different carbon sources

Cited by 27 publications

References 36 publications

Screening and characterisation of gamma-aminobutyric acid (GABA) producing lactic acid bacteria isolated from Thai fermented fish (Plaa-som) in Nong Khai and its application in Thai fermented vegetables (Som-pak)

Screening and characterisation of gamma-aminobutyric acid (GABA) producing lactic acid bacteria isolated from Thai fermented fish (Plaa-som) in Nong Khai and its application in Thai fermented vegetables (Som-pak)

Enhanced Production of Photosynthetic Pigments and Various Metabolites and Lipids in the Cyanobacteria Synechocystis sp. PCC 7338 Culture in the Presence of Exogenous Glucose

Metal Oxide Mediated Extracellular NADPH Regeneration Improves Ethanol Production by Engineered Synechocystis sp. PCC 6803

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