Glutamate Is a Wake-Active Neurotransmitter in Drosophila melanogaster

Zimmerman, John E.; Chan, May K.M.; Lenz, Olivia; Keenan, Brendan T.; Maislin, Greg; Pack, Allan I.

doi:10.1093/sleep/zsw046

Cited by 31 publications

(25 citation statements)

References 51 publications

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“…Glutamate neurotransmission among specific circadian clock neurons, on the other hand, has a timed sleep-promoting effect (Guo et al 2016). Broad manipulations of glutamatergic neurotransmission indicate that, on net, glutamate signaling promotes wakefulness especially at night (Zimmerman et al 2016). Homer proteins, adaptors for metabotropic glutamate receptors, also contribute to sleep homeostasis in both flies and mammals.…”

Section: Glutamatementioning

confidence: 99%

Molecular Mechanisms of Sleep Homeostasis in Flies and Mammals

Allada¹,

Cirelli

Sehgal

2017

Cold Spring Harb Perspect Biol

136

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Section: Glutamatementioning

confidence: 99%

Molecular Mechanisms of Sleep Homeostasis in Flies and Mammals

Allada¹,

Cirelli

Sehgal

2017

Cold Spring Harb Perspect Biol

136

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“…For sleep, it has previously been shown that artificially activating glutamatergic neurons in the brain leads to increased wakefulness, therefore inhibiting these neurons could result in increased sleep (Zimmerman et al 2017). We found genes that function in glutamate neurotransmitter release are repressed post-mating, in females that lack a germline, which could contribute to increased sleep.…”

Section: Discussionmentioning

confidence: 74%

“…Genes involved in GABA synthesis (Gad1, FC = 0.7) and transport of glutamate (VGlut, FC = 0.7) were both repressed by mating at three-days post-mating. Glutamatergic and GABAergic neurons are widespread in the Drosophila nervous system and have been associated with sleep and olfactory sensing (Liu and Wilson 2013;Zimmerman et al 2017). Adar (FC = 0.7), which is also included in this list of genes, has also been shown to effect sleep by repressing glutamatergic signaling (Robinson et al 2016).…”

Section: Discussionmentioning

confidence: 99%

TheDrosophilaPost-mating Response: Gene Expression and Behavioral Changes Reveal Perdurance and Variation in Cross-Tissue Interactions

Newell

Ray

Dalton

et al. 2020

G3 Genes|Genomes|Genetics

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Examining cross-tissue interactions is important for understanding physiology and homeostasis. In animals, the female gonad produces signaling molecules that act distally. We examine gene expression in Drosophila melanogaster female head tissues in 1) virgins without a germline compared to virgins with a germline, 2) post-mated females with and without a germline compared to virgins, and 3) post-mated females mated to males with and without a germline compared to virgins. In virgins, the absence of a female germline results in expression changes in genes with known roles in nutrient homeostasis. At one-and three-day(s) post-mating, genes that change expression are enriched with those that function in metabolic pathways, in all conditions. We systematically examine female post-mating impacts on sleep, food preference and re-mating, in the strains and time points used for gene expression analyses and compare to published studies. We show that post-mating, gene expression changes vary by strain, prompting us to examine variation in female re-mating. We perform a genome-wide association study that identifies several DNA polymorphisms, including four in/near Wnt signaling pathway genes. Together, these data reveal how gene expression and behavior in females are influenced by cross-tissue interactions, by examining the impact of mating, fertility, and genotype.

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“…The role of octopamine is not well defined as recent data showed that the effect of octopamine could be sleep-promoting rather than wake-promoting ( Deng et al, 2019 ). Finally, glutamate can promote sleep ( Tomita et al, 2015 ) or wakefulness ( Zimmerman et al, 2017 ) depending on the postsynaptic receptors. A similar effect is described for acetylcholine which promotes wakefulness by exciting l-LNvs when released from extra-retinal photoreceptors, Hofbauer–Buchner eyelets, and L2 neurons ( McCarthy et al, 2011 ; Muraro and Ceriani, 2015 ; Schlichting et al, 2016 ), and has a sleep-promoting effect when released from mushroom bodies ( Yi et al, 2013 ).…”

Section: Sleep In Drosophilamentioning

confidence: 99%

Better Sleep at Night: How Light Influences Sleep in Drosophila

2020

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Sleep-like states have been described in Drosophila and the mechanisms and factors that generate and define sleep-wake profiles in this model organism are being thoroughly investigated. Sleep is controlled by both circadian and homeostatic mechanisms, and environmental factors such as light, temperature, and social stimuli are fundamental in shaping and confining sleep episodes into the correct time of the day. Among environmental cues, light seems to have a prominent function in modulating the timing of sleep during the 24 h and, in this review, we will discuss the role of light inputs in modulating the distribution of the fly sleep-wake cycles. This phenomenon is of growing interest in the modern society, where artificial light exposure during the night is a common trait, opening the possibility to study Drosophila as a model organism for investigating shift-work disorders.

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Glutamate Is a Wake-Active Neurotransmitter in Drosophila melanogaster

Abstract: The results show the following: glutamate is indeed a wake-active neurotransmitter in Drosophila; there is a major time of day effect associated with loss of glutamatergic neurotransmission; and it is a major wake-active neurotransmitter in the nighttime.

Cited by 31 publications

References 51 publications

Molecular Mechanisms of Sleep Homeostasis in Flies and Mammals

Molecular Mechanisms of Sleep Homeostasis in Flies and Mammals

TheDrosophilaPost-mating Response: Gene Expression and Behavioral Changes Reveal Perdurance and Variation in Cross-Tissue Interactions

Better Sleep at Night: How Light Influences Sleep in Drosophila

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