Glutamate Is Excreted Across the Cytoplasmic Membrane through the NCgl1221 Channel ofCorynebacterium glutamicumby Passive Diffusion

L-Glutamate Secretion by the N-Terminal Domain of theCorynebacterium glutamicumNCgl1221 Mechanosensitive Channel

“…electrophysiolosical property of the N-terminal domain was similar to that of the full-length NCgl1221. 31) This clearly indicates that the N-terminal half of NCgl1221 functions as a mechanosenstive channel that can mediate L-glutamate secretion in response to changes in membrane tension.…”

“…The resulting plasmid, pJM116-Pxyl-286, was linearized with SacI and then integrated at the amyE locus of the B. subtilis mscL ykuT yfkC yhdY quadruple mutant strain. 31) The expression of the mutant NCgl1221 protein in B. subtilis was under the control of the xyl promoter. Electrophysiological analysis by the patchclamp method using B. subtilis giant provacuoles was done as described previously, except that the L-glutamate-containing buffer contained 15 mM L-glutamate instead of 20 mM L-glutamate.…”

“…We then examined the mechanosensitive channel activity of the N-terminal domain by the patch-clamp method using giant provacuoles prepared from the B. subtilis mscL ykuT yfkC yhdY quadruple mutant, 31) which expresses C. glutamicum NCgl1221-268. As shown in Fig.…”

“…Very recently, it was found that the NCgl1221 channel serves as a path for glutamate efflux. 31) The NCgl1221 protein has an N-terminal region similar to E. coli MscS and an additional C-terminal region of unknown function. In this study, we constructed a series of C-terminally truncated mutants of NCgl1221.…”

A role of the transcriptional regulator LldR (NCgl2814) in glutamate metabolism under biotin-limited conditions in Corynebacterium glutamicum

“…Specific mutations of NCgl1221 protein led to a constitutive L-glutamate secretion without any inducing treatments, while disruption of NCgl1221 greatly diminished L-glutamic acid secretion (Nakamura et al, 2007;Nakayama et al, 2012). Very recently, it has been demonstrated that the NCgl1221 channel does indeed serve as a path for glutamate efflux (Hashimoto et al, 2012).…”