Glutamate receptor 5/6/7-like and glutamate transporter-1-like immunoreactivity in the leech central nervous system

Thorogood, Maria Stella E.; Almeida, Vania W.; Brodfuehrer, Peter D.

doi:10.1002/(sici)1096-9861(19990315)405:3<334::aid-cne4>3.0.co;2-i

Cited by 11 publications

(7 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Concerning NMDA receptors in the leech, Schlue's group found that bath application of NMDA had no effect on the membrane potential, intracellular Na + activity, and intracellular K + activity of Retzius cells (Do¨rner et al 1990). Brodfuehrer and his students, using a monoclonal antibody against the mammalian NMDA-receptor subunit NR1, found no immunoreactivity in the leech ganglion (Thorogood et al 1999). Bath application of APV at 50 lM did not block P to AP synaptic transmission (Wessel et al 1999).…”

Section: Introductionmentioning

confidence: 97%

“…Non-NMDA receptors have been identified in leech neurons and glial cells (Do¨rner et al 1990;Dierkes et al 1996;Thorogood et al 1999;Wessel et al 1999;Baccus et al 2000). Concerning NMDA receptors in the leech, Schlue's group found that bath application of NMDA had no effect on the membrane potential, intracellular Na + activity, and intracellular K + activity of Retzius cells (Do¨rner et al 1990).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evidence against the presence of NMDA receptors at a central glutamatergic synapse in leeches

2002

Invertebrate Neuroscience

View full text Add to dashboard Cite

The N-methyl- D-aspartate (NMDA) receptor, able to detect the coincidence of pre- and postsynaptic events, is considered to be the molecular analogue of associative learning. Associative learning is well known in leeches, particularly for reflexive shortening. The neuronal circuits underlying shortening have been documented and include neurons that release glutamate. Is this type of learning in leeches also mediated by NMDA receptors? The synapse between the P sensory neuron and the motoneuron-like AP cell was examined and: (1) NMDA failed to elicit a response in the AP cell, (2) the NMDA receptor antagonist 2-amino-5-phosphopentanoic acid affected synaptic transmission only at high, non-specific levels, and (3) the antagonist for the glycine-binding site 7-chloro-kynurenic acid at 20 microM did not inhibit transmission. Therefore, there are evidently no NMDA receptors at the P to AP synapse, suggesting other mechanisms of associative learning in leeches.

show abstract

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Evidence against the presence of NMDA receptors at a central glutamatergic synapse in leeches

2002

Invertebrate Neuroscience

View full text Add to dashboard Cite

show abstract

“…The GluR5–7 was described to be made against a sequence of extracellular domain of the GlurR5 receptor subunit (aa: 233–518) and recognizes kainite receptor subtypes GluR5, GlurR6, and GlurR7 subunits (Huntley et al, ). GluR5–7 immunoreactivity has also previously been identified throughout the neuropile of another annelid, the leech Hirudo (Thorogood, Almeida, & Brodfuehrer, ). The GluR2,3 antibody () was characterized by the manufacturer (Table ) and was specific to both phosphorylated and unphosphorylated GluR2,3 subunits on the Serine 880/891 of mature human brain tissue.…”

Section: Methodsmentioning

confidence: 79%

“…The GluR2,3 antibody () was characterized by the manufacturer (Table ) and was specific to both phosphorylated and unphosphorylated GluR2,3 subunits on the Serine 880/891 of mature human brain tissue. A polyclonal GluR2,3 antiserum against a C‐terminal domain (aa: 850–862) of AMPA receptor subtype was also reactive in leech (Thorogood et al, ). Antibodies against the N‐Methyl‐D‐Asparate Receptor‐1 (NR1; ) were previously generated from the extracellular loop between transmembrane domains 3 and 4 (clone 54.1) (Siegel et al, ).…”

Section: Methodsmentioning

confidence: 99%

Coupled sensory interneurons mediate escape neural circuit processing in an aquatic annelid worm, Lumbriculus variegatus

Lybrand

Martinez-Acosta

Zoran

2019

J of Comparative Neurology

View full text Add to dashboard Cite

The interneurons associated with rapid escape circuits are adapted for fast pathway activation and rapid conduction. An essential aspect of fast activation is the processing of sensory information with limited delays. Although aquatic annelid worms have some of the fastest escape responses in nature, the sensory networks that mediate their escape behavior are not well defined. Here, we demonstrate that the escape circuit of the mud worm, Lumbriculus variegatus, is a segmentally arranged network of sensory interneurons electrically coupled to the central medial giant fiber (MGF), the command-like interneuron for head withdrawal. Electrical stimulation of the body wall evoked fast, short-duration spikelets in the MGF, which we suggest are the product of intermediate giant fiber activation coupled to MGF collateral dendrites. Since these contact sites have immunoreactivity with a glutamate receptor antibody, and the glutamate receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dion abolishes evoked MGF responses, we conclude that the afferent pathway for MGFmediated escape is glutamatergic. This electrically coupled sensory network may facilitate rapid escape activation by enhancing the amplitude of giant axon depolarization.

show abstract

“…At higher concentrations, KA acting at AMPA receptors, which have higher mol·l -1 affinities (30-100·mol·l -1 ) (Hollmann et al, 1989), might be responsible for depolarization and the increased frequency of firing. Immunoreactivity to a vertebrate GluR5/6/7 antibody has been observed in both Helisoma buccal ganglia (Marya et al, 2007) and leech CNS (Thorogood et al, 1999). However, B5 was not among the neurons labeled in Helisoma.…”

Section: Excitatory Glutamate Receptors On B5 B19 and B27 Neuronsmentioning

confidence: 98%

Pharmacology of ionotropic and metabotropic glutamate receptors on neurons involved in feeding behavior in the pond snail, Helisoma trivolvis

Scannell

Dell'Ova

Quinlan

et al. 2008

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARYGlutamate is a key regulatory neurotransmitter in the triphasic central pattern generator controlling feeding behavior in the pond snail, Helisoma trivolvis. It excites phase two motor neurons while inhibiting those in phases one and three. However, the receptors that mediate this regulation are only partially characterized. The purpose of these experiments was to further characterize the glutamate receptors on three buccal neurons modulated by glutamate. Intracellular recordings from B5, B19 and B27 neurons were taken during the perfusion of isolated buccal ganglia with agonists that are selective for different vertebrate glutamate receptors. The firing rate of all three neurons was inhibited in a dose-dependent manner by glutamate, including that of B27, a phase 2 motor neuron known to be excited by glutamate in vivo. Quisqualate also reduced the firing rate in all three neurons, and (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (ACPD), a relatively non-selective metabotropic glutamate receptor (mGluR) agonist, reduced the firing rate in B5 neurons, but not in B19 or B27 neurons. Agonists selective for vertebrate group I, II and III mGluRs did not affect the firing rate in any of the Helisoma buccal neurons tested, suggesting that mGluR agonist binding sites on these neurons do not closely resemble those on any vertebrate mGluR subtypes. An increase in frequency of action potentials was observed in all three cell types in the presence of 100·mol·l -1 kainate (KA), suggesting the presence of excitatory (S)-␣-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)/KA-like receptors. However, electrotonic coupling between B19 and B27 neurons, and a lack of effect of KA on isolated B19 neurons suggest the excitatory effects of KA on this neuron are indirect. These findings suggest the presence of multiple glutamate receptor subtypes in molluscan neurons that do not always resemble vertebrate receptors pharmacologically.

show abstract

Glutamate receptor 5/6/7-like and glutamate transporter-1-like immunoreactivity in the leech central nervous system

Cited by 11 publications

References 55 publications

Evidence against the presence of NMDA receptors at a central glutamatergic synapse in leeches

Evidence against the presence of NMDA receptors at a central glutamatergic synapse in leeches

Coupled sensory interneurons mediate escape neural circuit processing in an aquatic annelid worm, Lumbriculus variegatus

Pharmacology of ionotropic and metabotropic glutamate receptors on neurons involved in feeding behavior in the pond snail, Helisoma trivolvis

Contact Info

Product

Resources

About