Glutamate receptor-mediated calcium responses in acutely isolated hippocampal astrocytes

Cai, Zhaohui; Kimelberg, Harold K.

doi:10.1002/(sici)1098-1136(199712)21:4<380::aid-glia5>3.0.co;2-7

Cited by 35 publications

(24 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistently, Cai and Kimelberg [95] found no response to NMDA (100 μM) in GFAP+ acute isolated astrocytes from rat hippocampus. Notably, Gottlieb and Matute [96] in the same year found by IHC the expression of GluN2A and GluN2B subunits in rat hippocampus reactive astrocytes after transient ischemia.…”

Section: After Casupporting

confidence: 52%

NMDA Receptors in Astroglia: Chronology, Controversies, and Contradictions from a Complex Molecule

Balderas¹,

GonzálezHernández²

2018

Astrocyte - Physiology and Pathology

View full text Add to dashboard Cite

The neurocentric theory dismissed for decades the role of glia in information handling within the central nervous system (CNS). Nevertheless, almost 3 decades ago, this started to change and today astrocytes are considered relevant players for this function. Astrocytes "listen" to neuronal communication, regulate it, and respond at the cellular and synctitial level. Ionotropic glutamate NMDA receptor (NMDAR) is critical in CNS. It mediates synaptic neuronal communication and it is involved in different mechanisms. However, NMDAR is also expressed by astrocytes, but its functional role in these cells has not been deeply investigated and has been a matter of debate in the last decades. In this chapter, we briefly outline NMDAR intracellular transduction pathways initiated by Ca 2+ flux. Then, we review chronologically NMDAR expression and function in astrocytes that have been a source of controversies and apparent contradictions. Finally, some insights are presented regarding NMDAR in astrocytes in the context of the tripartite synapse concept and the recently described Ca 2+ fluxindependent metabotropic-like NMDAR function in astrocytes. Given the complex molecular nature of NMDAR, its critical role, and the relevance of astrocytes, the study of astrocytic NMDAR promises to provide further understanding of CNS physiology and pathology.

show abstract

Section: After Casupporting

confidence: 52%

NMDA Receptors in Astroglia: Chronology, Controversies, and Contradictions from a Complex Molecule

Balderas¹,

GonzálezHernández²

2018

Astrocyte - Physiology and Pathology

View full text Add to dashboard Cite

show abstract

“…These ambivalent results might be due to the inherent complexity of the problem, especially when the neurons in the surrounding tissue have to be taken into account. Acutely isolated hippocampal astrocytes should help to overcome such a problem; however, NMDA complemented with glycine in Mg 2+ -free medium did not evoke any increases in membrane currents [72] or in the extent of Ca 2+ elevation [73], indicating that acutely isolated hippocampal astrocytes do not possess any functional NMDA receptors under physiological conditions. On the other hand, the impact of the isolation procedure should be also considered.…”

Section: Astrocytic Nmda Receptorsmentioning

confidence: 99%

NMDA Receptors in Glial Cells: Pending Questions

Džamba¹,

Honsa²,

Andĕrová

2013

View full text Add to dashboard Cite

Glutamate receptors of the N-methyl-D-aspartate (NMDA) type are involved in many cognitive processes, including behavior, learning and synaptic plasticity. For a long time NMDA receptors were thought to be the privileged domain of neurons; however, discoveries of the last 25 years have demonstrated their active role in glial cells as well. Despite the large number of studies in the field, there are many unresolved questions connected with NMDA receptors in glia that are still a matter of debate. The main objective of this review is to shed light on these controversies by summarizing results from all relevant works concerning astrocytes, oligodendrocytes and polydendrocytes (also known as NG2 glial cells) in experimental animals, further extended by studies performed on human glia. The results are divided according to the study approach to enable a better comparison of how findings obtained at the mRNA level correspond with protein expression or functionality. Furthermore, special attention is focused on the NMDA receptor subunits present in the particular glial cell types, which give them special characteristics different from those of neurons – for example, the absence of Mg2+ block and decreased Ca2+ permeability. Since glial cells are implicated in important physiological and pathophysiological roles in the central nervous system (CNS), the last part of this review provides an overview of glial NMDA receptors with respect to ischemic brain injury.

show abstract

“…Even more importantly, it seems that the mechanism by which intracellular Ca 2ϩ increases during neuronal activity (in identified complex astrocytes in vivo) is via metabotropic glutamate receptors, rather than voltage-gated ion channels (Carmignoto et al, 1998). It appears that both GFAPϩ and negative astrocytes possess metabotropic glutamate receptors (Porter and McCarthy, 1996;Cai and Kimelberg, 1997;Pasti et al, 1997).…”

Section: Monitors Of Synaptic Activitymentioning

confidence: 99%

Controversy surrounding the existence of discrete functional classes of astrocytes in adult gray matter

Walz

2000

Glia

133

View full text Add to dashboard Cite

Glutamate receptor-mediated calcium responses in acutely isolated hippocampal astrocytes

Cited by 35 publications

References 51 publications

NMDA Receptors in Astroglia: Chronology, Controversies, and Contradictions from a Complex Molecule

NMDA Receptors in Astroglia: Chronology, Controversies, and Contradictions from a Complex Molecule

NMDA Receptors in Glial Cells: Pending Questions

Controversy surrounding the existence of discrete functional classes of astrocytes in adult gray matter

Contact Info

Product

Resources

About