Glutamic Pyruvate Transaminase Phenotypes in Polycythaemia Rubra Vera

Kirkland, David; Welch, S.G.; Povey, S.; Najfeld, Vesna; Price, Derek J.; Lawler, Sylvia D.

doi:10.1111/j.1365-2141.1980.tb05910.x

Cited by 9 publications

(2 citation statements)

References 16 publications

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“…Trisomy 8 is observed in cases of chronic myeloid leukemia (CML; Lindquist et al 1978;Kohno et al 1979;Puchkova et al 1983;Haas et al 1984) or polycythemia vera (Shabtai et al 1978;Kirkland et al 1980;Berger et al 1984). In these cases, the presence of the extra chromosome allows easy identification of the neoplastic cells, thereby facilitating assessment of their frequency.…”

Section: Introductionmentioning

confidence: 97%

Labeling of the centromeric region on human chromosome 8 by in situ hybridization

1991

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Probe DNA that binds preferentially to the centromeric region of human chromosomes 8 was synthesized. Alpha satellite probe DNA molecules were selectively amplified from sorter-purified human chromosomes 8 by in vitro DNA amplification using the polymerase chain reaction (PCR). Probe labeling was performed during PCR by incorporation of biotinylated deoxyuridine. In situ hybridization of unpurified probe DNA comprised of alpha satellite monomer and higher molecular weight DNA fragments with metaphase chromosome spreads showed binding to the centromeric regions of numerous chromosomes. However, blocking with unlabeled total human alphoid DNA dramatically reduced crosshybridization to chromosomes other than 8. Under these conditions, the degenerate probe DNA allowed unambiguous visualization of domains occupied by centromeric DNA of chromosome 8 in metaphase spreads and interphase cell nuclei, thus greatly facilitating the detection of numerical chromosome aberrations in tumor cells. In situ hybridization of size-fractionated alpha satellite DNA identified the monomeric fraction as the major cause of crosshybridization. Alpha satellite dimers and higher molecular weight DNA fragments showed relatively high specificity for human chromosomes 8.

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Section: Introductionmentioning

confidence: 97%

Labeling of the centromeric region on human chromosome 8 by in situ hybridization

1991

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“…2 Since then, its association with polycythemia vera has been confirmed. 3 " 12 It has been observed that the bone marrow can acquire 20q-in polycythemia vera before therapy, following therapy, or with progression to postpolycythemic myeloid metaplasia and acute leukemia. 4 ' 613 "' 5 Furthermore, 20q-has been described in other chronic myeloproliferative disorders, including agnogenic myeloid metaplasia 31014 ' 6 " 18 and essential thrombocythemia, 3101619 but not Ph+ chronic myeloid leukemia.…”

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confidence: 99%