Glutamic γ-semialdehyde in arginine and proline synthesis of neurospora: A mutant-tracer analysis

Vogel, Ruth H.; Kopac, M. J.

doi:10.1016/0006-3002(59)90192-1

Cited by 39 publications

(18 citation statements)

References 11 publications

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“…The existence of the urea cycle in Neurospora crassa has been postulated because of the presence of arginase and the enzymes involved in arginine biosynthesis (Srb & Horowitz, 1944). Further, exogenous arginine, ornithine or citrulline can replace proline in a mutant that requires the latter for growth, although it can synthesize arginine (Vogel & Kopac, 1959), which indicates that arginase can utilize exogenous but not endogenous arginine. Wagner & Bergquist (1963) found that the synthesis of isoleucine requires, not only the enzymes that participate in the biosynthetic path, but their physical connexion.…”

Section: Discussionmentioning

confidence: 99%

The Regulation of Urea-Biosynthesis Enzymes in Vertebrates

Mora¹,

Martuscelli²,

Pineda³

et al. 1965

Biochemical Journal

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1. Carbamoyl phosphate synthetase, ornithine transcarbamoylase, the arginine-synthetase system and arginase were measured in the livers of ammoniotelic, ureotelic and uricotelic animals. The chelonian reptiles, whose nitrogen excretory patterns vary according to the habitat, and the Mexican axolotl, a neotenic species, were also studied. 2. The levels of the activities of the first three enzymes mentioned correlate with the amount of nitrogen excreted as urea. 3. The terrestrial turtle, which excretes mainly uric acid, maintains a high arginase activity but has very low levels of the activities of the other three enzymes. 4. The first three enzymes of the urea cycle vary in the phylogenic scale in a co-ordinated manner, which suggests that they are under the same regulatory mechanism. 5. Urea formation from endogenous arginine in vitro has a low efficiency in the Mexican axolotl. 6. The induction of metamorphosis in the Mexican axolotl by the administration of l-tri-iodothyronine, which causes a shift from ammonio-ureotelism to complete ureotelism, is accompanied by an increase mainly in carbamoyl phosphate synthetase and also by an improvement in the efficiency of hydrolysis of endogenous arginine in vitro to give urea. 7. The results obtained by differential centrifugation of the urea-cycle enzymes in rat and Mexican-axolotl livers are presented. The location requirements for the integration of a metabolic cycle are discussed.

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Section: Discussionmentioning

confidence: 99%

The Regulation of Urea-Biosynthesis Enzymes in Vertebrates

Mora¹,

Martuscelli²,

Pineda³

et al. 1965

Biochemical Journal

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“…Labeled ornithine and arginine will label proline (Lignowski and Splittstoesser, 1971;Jones and Boulter, 1968;Duranton and Wurtz, 1965;Mestichelli et al, 1979;Coleman and Hegarty, 1957;Brown and Fowden, 1966;Liebisch and Schutte, 1967;Vogel and Kopac, 1959) and in higher plants this labeling is enhanced by water stress (Boggess et al, 1976b;Boggess and Stewart, 1976;Stewart and Boggess, 1977). These observations can be explained as the result of arginase activity, aminotransferase activity with an equilibrium away from ornithine (Fincham, 1953), and reduction of P5C and P2C to proline.…”

Section: Interconnections Between Metabolic Pathways Of Arginine mentioning

confidence: 99%

“…Mitochondrial preparations from several higher plants (Stewart and Lai, 1974;Boggess et al, 1975) oxidize P5C but not P2C to glutamic acid. In Neurospora, exogenous ornithine had ready access to the proline biosynthetic pathway while endogenous ornithine did not (Vogel and Kopac, 1959). (22) A disagreement between labeling and enzymatic data as in conversion of ornithine to proline often occurs (see Reed and Lukens, 1966) because it is difficult to prove the pathway of in vivo transformations by the presence of enzymes.…”

Section: Interconnections Between Metabolic Pathways Of Arginine mentioning

confidence: 99%

Arginine Synthesis, Proline Synthesis, and Related Processes

Thompson¹

1980

Amino Acids and Derivatives

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“…In a typical experiment the growth rate was exponential up to about 40 hr. Growth and synthesis of bacterial-cell protein proceeded actively for more than 70 hr. The initial gramicidin S content was very low; at about 50 hr the rate of production of the antibiotic increased, and in 72 hr the peptide attained a concentration of 150 mg/l.…”

Section: B Brevismentioning

confidence: 99%