Glutamine requirements in the generation of lymphokine-activated killer cells

António, José; Spagnoli, Giulio C.; Hörig, Heidi; Babst, Reto; Bremen, K. Von; Harder, F.; Heberer, Michael

doi:10.1016/0261-5614(94)90009-4

Cited by 41 publications

(19 citation statements)

References 32 publications

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“…Glutamine in in vitro studies has been shown to in¯uence the proliferation of lymphocytes (Ardawi and Newsholme 1983;Newsholme and Parry Billings 1990;Parry Billings et al 1990Rohde et al 1995) and the LAK cell activity (Juretic et al 1994;Rohde et al 1995). Furthermore, there has been found to be a correlation between the exercise-induced decline in serum glutamine and LAK cell activity (Rohde et al 1996).…”

Section: Discussionmentioning

confidence: 97%

“…Glutamine has been shown to be important for optimal in vitro lymphocyte proliferation and generation of lymphokine activated killer (LAK) cell activity (Juretic et al 1994;Rohde et al 1995Rohde et al , 1996Rowbottom et al 1996). The plasma concentration of glutamine has been found to decline after intense exercise (Parry Billings et al 1992;Keast et al 1995;Rohde et al 1996) and it has been suggested that this may play a role in the impaired lymphocyte function (Newsholme 1994).…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Competitive sustained exercise in humans, lymphokine activated killer cell activity, and glutamine ? an intervention study

Rohde

Asp

MacLean

et al. 1998

European Journal of Applied Physiology

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This study examined whether oral glutamine supplementation abolishes some of the exercise-induced changes in lymphocyte functions following long-term intense exercise. A group of 16 marathon runners participating in The Copenhagen Marathon 1996 were placed randomly in either a placebo (n = 7) or a glutamine receiving group (n = 9). Each subject received four doses of either placebo or glutamine (100 mg x kg(-1)) administered at 0, 30, 60, and 90-min post-race. In the placebo group the plasma glutamine concentrations were lower than pre-race values during the post-exercise period [mean 647 (SEM 32) compared to 470 (SEM 22) micromol x 1(-1) 90-min post-race, P < 0.05] whereas glutamine supplementation maintained the plasma glutamine concentration (at approximately 750 micromol x 1(-1)). Glutamine supplementation in vivo had no effect on the lymphokine activated killer (LAK) cell activity, the proliferative responses or the exercise-induced changes in concentrations or percentages of any of the leucocyte subpopulations examined. Glutamine addition in in vitro studies enhanced the proliferative response in both groups. These data would suggest that decreased plasma glutamine concentrations post-exercise are not responsible for exercise-induced decrease in LAK activity and that the influence of glutamine in vitro is not dependent on the plasma glutamine concentration at the time of sampling.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 98%

Competitive sustained exercise in humans, lymphokine activated killer cell activity, and glutamine ? an intervention study

Rohde

Asp

MacLean

et al. 1998

European Journal of Applied Physiology

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“…All blood samples were drawn into heparinized vacutainers, refrigerated at 4°C and processed within 12 h as previously described [19].…”

Section: Methodsmentioning

confidence: 99%

Alterations in Lymphocyte Phenotype of Infected Preterm Newborns

Juretić

António²,

Užarević³

et al. 2001

Neonatology

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The cord blood lymphocytes from 44 premature newborns were analyzed using two-color monoclonal antibodies and flow cytometry. Depending on whether or not there was an infection at birth, the newborns were divided into two groups and the immunophenotypes of infected and uninfected newborns were compared. The percentage of T lymphocytes (CD3+) was significantly lower in the infected prematures. The percentage of both helper and cytotoxic T lymphocytes was lower. The proportion of activated T lymphocytes, cytotoxic non-MHC-restricted T lymphocytes, NK cells and B lymphocytes did not differ between the group of infected and the group of uninfected prematures. The percentage of memory helper T lymphocytes (CD45RO+CD4+) was very low in premature newborns regardless of whether or not they were infected and could not be used as a marker of bacterial infection at this age.

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“…Glutamine directly enhances the function of T lymphocytes, B lymphocytes, lymphokineactivated killer cells, and macrophages. [17][18][19][20] Finally, glutamine serves as a primary energy substrate for enterocytes and plays an important role in proliferation of the intestinal mucosa and other cell types. [20][21][22][23] Nucleotides (RNA) Nucleotides (purines and pyrimidines) stimulate the immune system in several ways.…”

Section: Argininementioning

confidence: 99%