Glutamine synthetase gene of Bacillus subtilis

Fisher, Susan H.; Rosenkrantz, M S; Sonenshein, Abraham L.

doi:10.1016/0378-1119(84)90018-0

Cited by 56 publications

(49 citation statements)

References 28 publications

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“…This concentration closely matches the intracellular glutamine levels of B. subtilis cells grown in the presence of excess nitrogen (18). When glutamine was not present, both the wild-type and mutant enzymes had similar abilities in blocking TnrA DNA binding (Fig.…”

Section: Assaysupporting

confidence: 69%

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A Feedback-resistant Mutant of Bacillus subtilis Glutamine Synthetase with Pleiotropic Defects in Nitrogen-regulated Gene Expression

Wray

Fisher

2005

Journal of Biological Chemistry

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The Bacillus subtilis TnrA transcription factor regulates gene expression during nitrogen-limited growth. When cells are grown with excess nitrogen, feedback-inhibited glutamine synthetase forms a protein-protein complex with TnrA and prevents TnrA from binding to DNA. A mutation in glutamine synthetase with a phenylalanine replacement at the Ser-186 residue (S186F) was isolated by screening for B. subtilis mutants with constitutive TnrA activity. Although S186F glutamine synthetase has kinetic properties that are similar to the wild-type protein, the S186F enzyme is resistant to feedback inhibition by glutamine and AMP. Ligand binding experiments revealed that the S186F protein had a lower affinity for glutamine and AMP than the wild-type enzyme. S186F glutamine synthetase was defective in its ability to block DNA binding by TnrA in vitro. The properties of the feedback-resistant S186F mutant support the model in which the feedback-inhibited form of glutamine synthetase regulates TnrA activity in vivo.

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Section: Assaysupporting

confidence: 69%

“…The ␥-glutamyltransferase reverse reaction is inhibited by AMP, alanine, glycine, serine, tryptophan, and histidine (16). Posttranslational modification of B. subtilis GS has not been observed (17,18).…”

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confidence: 99%

A Feedback-resistant Mutant of Bacillus subtilis Glutamine Synthetase with Pleiotropic Defects in Nitrogen-regulated Gene Expression

Wray

Fisher

2005

Journal of Biological Chemistry

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“…The concentration of GS protein in B. subtilis is regulated at the transcriptional level in response to the nitrogen source available in the medium (6,7,21). The gene encoding GS, glnA, lies in a dicistronic operon in which it is preceded by glnR (25), a gene whose product is required for negative regulation of the operon (20).…”

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confidence: 99%

Autogenous regulation of the Bacillus subtilis glnRA operon

Brown

Sonenshein

1996

J Bacteriol

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“…In the case of the hut operon and citB, amino acid repression was shown to be independent of carbon catabolite repression (2,14) and glutamine synthetase (2). The second type of control requires the product of glnA, glutamine synthetase (11). In this form of regulation, expression is sensitive to nitrogen availability.…”

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confidence: 99%

Nitrogen regulation of nasA and the nasB operon, which encode genes required for nitrate assimilation in Bacillus subtilis

et al. 1995

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The divergently transcribed nasA gene and nasB operon are required for nitrate and nitrite assimilation in Bacillus subtilis. The ␤-galactosidase activity of transcriptional lacZ fusions from the nasA and nasB promoters was high when cells were grown in minimal glucose medium containing poor nitrogen sources such as nitrate, proline, or glutamate. The expression was very low when ammonium or glutamine was used as the sole nitrogen source. The repression of the genes during growth on good sources of nitrogen required wild-type glutamine synthetase (GlnA), but not GlnR, the repressor of the glnRA operon. Primer extension analysis showed that the ؊10 region of each promoter resembles those of A -recognized promoters. Between the divergently oriented nasA and nasB promoters is a region of dyad symmetry. Mutational analysis led to the conclusion that this sequence is required in cis for the activation of both nasA and nasB. The derepression of these genes in a glnA mutant also required this sequence. These results suggest that an unidentified transcriptional activator and glutamine synthetase function in the regulation of nasA and the nasB operon.

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Glutamine synthetase gene of Bacillus subtilis

Cited by 56 publications

References 28 publications

A Feedback-resistant Mutant of Bacillus subtilis Glutamine Synthetase with Pleiotropic Defects in Nitrogen-regulated Gene Expression

A Feedback-resistant Mutant of Bacillus subtilis Glutamine Synthetase with Pleiotropic Defects in Nitrogen-regulated Gene Expression

Autogenous regulation of the Bacillus subtilis glnRA operon

Nitrogen regulation of nasA and the nasB operon, which encode genes required for nitrate assimilation in Bacillus subtilis

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