Glutathione and GSH-dependent enzymes in bronchoalveolar lavage fluid cells in response to ozone

Boehme, D.S.; Hotchkiss, Jon A.; Henderson, Rogene F.

doi:10.1016/0014-4800(92)90021-3

Cited by 45 publications

(22 citation statements)

References 30 publications

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“…On the other hand, rhinovirus infection of bronchial epithelial cells has been shown to induce ROS formation (35) and to increase SOD 1 expression and total SOD activity at early time points of infection, with no changes in SOD 2, catalase, and GPx (36), although AOE expression/activity was investigated only at 6 hours after infection. Regarding other noninfectious conditions, an increase in antioxidant defenses has been shown to occur in certain pulmonary diseases with significant oxidant burden, such as exposure to hyperoxia (37), ozone (38), and cigarette smoke (39), although decreased antioxidant expression and/or activity has been reported in other respiratory acute and chronic inflammatory disease, such as asthma and COPD (40)(41)(42)(43). Reduced SOD and catalase activity has been shown in airway epithelial cells and/or bronchoalveolar lavage obtained from patients with asthma (40)(41)(42).…”

Section: Discussionmentioning

confidence: 99%

Viral-mediated Inhibition of Antioxidant Enzymes Contributes to the Pathogenesis of Severe Respiratory Syncytial Virus Bronchiolitis

Hosakote

Jantzi

Esham

et al. 2011

Am J Respir Crit Care Med

149

161

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Rationale : Respiratory syncytial virus (RSV) is a major cause of lower respiratory tract infections in children, for which no specific treatment or vaccine is currently available. We have previously shown that RSV induces reactive oxygen species in cultured cells and oxidative injury in the lungs of experimentally infected mice. The mechanism(s) of RSV-induced oxidative stress in vivo is not known. Objectives : To measure changes of lung antioxidant enzymes expression/activity and activation of NF-E2-related factor 2 (Nrf2), a transcription factor that regulates detoxifying and antioxidant enzyme gene expression, in mice and in infants with naturally acquired RSV infection. Methods : Superoxide dismutase 1 (SOD 1), SOD 2, SOD 3, catalase, glutathione peroxidase, and glutathione S-transferase, as well as Nrf2 expression, were measured in murine bronchoalveolar lavage, cell extracts of conductive airways, and/or in human nasopharyngeal secretions by Western blot and two-dimensional gel electrophoresis. Antioxidant enzyme activity and markers of oxidative cell injury were measured in either murine bronchoalveolar lavage or nasopharyngeal secretions by colorimetric/immunoassays. Measurements and Main Results : RSV infection induced a significant decrease in the expression and/or activity of SOD, catalase, glutathione S-transferase, and glutathione peroxidase in murine lungs and in the airways of children with severe bronchiolitis. Markers of oxidative damage correlated with severity of clinical illness in RSVinfected infants. Nrf2 expression was also significantly reduced in the lungs of viral-infected mice. Conclusions : RSV infection induces significant down-regulation of the airway antioxidant system in vivo, likely resulting in lung oxidative damage. Modulation of oxidative stress may pave the way toward important advances in the therapeutic approach of RSV-induced acute lung disease.

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Section: Discussionmentioning

confidence: 99%

Viral-mediated Inhibition of Antioxidant Enzymes Contributes to the Pathogenesis of Severe Respiratory Syncytial Virus Bronchiolitis

Hosakote

Jantzi

Esham

et al. 2011

Am J Respir Crit Care Med

149

161

View full text Add to dashboard Cite

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“…Another thiol, N-acetylcysteine, also has found use as an approach to augment RTLF antioxidant capabilities and in some studies appears to have produced small rises in RTLF GSH (83). However, several conditions associated with inflammatory airway responses, including cigarette smoking (51,52), asthma (84), and experimental exposure to 03 (85), are associated with high levels of RTLF GSH. Perhaps this represents an adaptive response to the oxidant stress of cigarette smoking or the inflammatory response (52), or perhaps it occurs subsequent to GSH release from inflammatory cells or RTECs that have been injured or shedded.…”

Section: Comments About Specific Antioxidant Substances In Rtlfsmentioning

confidence: 99%

Oxidants, antioxidants, and respiratory tract lining fluids.

Cross

Vliet

O’Neill

et al. 1994

Environ Health Perspect

222

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Respiratory tract lining fluids (RTLFs) are a heterogeneous group of substances covering the respiratory tract epithelial cells (RTECs) from nasal mucosa to alveoli. Antioxidants contained in the RTLFs can be expected to provide an initial defense against inhaled environmental toxins. The major antioxidants in RTLF include mucin, uric acid, protein (largely albumin), ascorbic acid, and reduced glutathione (GSH). RTLF antioxidants can be augmented by such processes as transudation/exudation of plasma constituents; RTEC secretory processes, including glandular mucus secretion; and cellular antioxidants derived from lysis of RTECs and of inflammatory cells. The antioxidant composition of RTLFs and their role in modulating normal and pathophysiologic RTEC functions under conditions of oxidative stress are yet to be fully characterized. -Environ Health Perspect 102(Suppl 10): 185-191 (1994)

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“…In vitro und in vivo werden durch Ozon-Exposition zahlreiche Enzyme induziert, die zu den Schutzmechanismen gegen oxidativen Streß gehören. Zu diesen zählen die Superoxid-Dismutase, die Glutathionperoxidase, die Glutathionreduktase und die Katalase sowie das Glutathion (Boehme et al 1992;Rahman et al 1991). Daneben werden Phospholipasen und Faktoren, die bei der Lipidperoxidation induziert werden, aktiviert (Wright et al 1994).…”

Section: Wirkungsmechanismusunclassified

Ozon [MAK Value Documentation in German language, 1995]

2012

The MAK‐Collection for Occupational Health and Safety

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Veröffentlicht in der Reihe Gesundheitsschädliche Arbeitsstoffe , 21. Lieferung, Ausgabe 1995 Der Artikel enthält folgende Kapitel: Allgemeiner Wirkungscharakter Wirkungsmechanismus Toxikokinetik Erfahrungen beim Menschen Einmalige inhalative Exposition Wiederholte inhalative Exposition Tierexperimentelle Befunde und in‐vitro‐Untersuchungen Akute und subakute inhalative Toxizität Subchronische und chronische Toxizität Allergene Wirkung Reproduktionstoxizität Genotoxizität Kanzerogenität Wirkung auf das Immunsystem Bewertung

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Glutathione and GSH-dependent enzymes in bronchoalveolar lavage fluid cells in response to ozone

Cited by 45 publications

References 30 publications

Viral-mediated Inhibition of Antioxidant Enzymes Contributes to the Pathogenesis of Severe Respiratory Syncytial Virus Bronchiolitis

Viral-mediated Inhibition of Antioxidant Enzymes Contributes to the Pathogenesis of Severe Respiratory Syncytial Virus Bronchiolitis

Oxidants, antioxidants, and respiratory tract lining fluids.

Ozon [MAK Value Documentation in German language, 1995]

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