Glutathione Concentration during Maturation and after Fertilization in Pig Oocytes: Relevance to the Ability of Oocytes to Form Male Pronucleus1

Yoshida, Mitsutoshi; Ishigaki, Koji; Nagai, Taku; Chikyu, Mikio; Pursel, V. G.

doi:10.1095/biolreprod49.1.89

Cited by 333 publications

(248 citation statements)

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“…Because BDNF treatment enhanced oocyte glutathione content, these findings suggest that BDNF could play a role in successful fertilization. Our data are consistent with earlier studies showing a correlation between glutathione levels in mature pig oocytes and their ability to form male pronuclei after fertilization (19). Thus, ovarian BDNF could activate separate downstream pathways in the oocyte to facilitate nuclear maturation (first polar body extrusion) as well as fertilization (glutathione content) and early embryo development.…”

Section: Discussionsupporting

confidence: 93%

Ovarian brain-derived neurotrophic factor (BDNF) promotes the development of oocytes into preimplantation embryos

Kawamura

Mulders

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

168

126

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Optimal development of fertilized eggs into preimplantation embryos is essential for reproduction. Although mammalian oocytes ovulated after luteinizing hormone (LH) stimulation can be fertilized and promoted into early embryos in vitro, little is known about ovarian factors important for the conditioning of eggs for early embryo development. Because LH interacts only with ovarian somatic cells, its potential regulation of oocyte functions is presumably mediated by local paracrine factors. We performed DNA microarray analyses of ovarian transcripts and identified brainderived neurotrophic factor (BDNF) secreted by granulosa and cumulus cells as an ovarian factor stimulated by the preovulatory LH surge. Ovarian BDNF acts on TrkB receptors expressed exclusively in oocytes to enhance first polar body extrusion of oocytes and to promote the in vitro development of zygotes into preimplantation embryos. Furthermore, in vivo treatment with a Trk receptor inhibitor suppressed first polar body extrusion and the progression of zygotes into blastocysts. Thus, ovarian BDNF is important to nuclear and cytoplasmic maturation of the oocyte, which is essential for successful oocyte development into preimplantation embryos. Treatment with BDNF could condition the cultured oocytes for optimal progression into the totipotent blastocysts.early embryo development ͉ gonadotropins ͉ ovulation I n vertebrates, rupture of ovarian follicles and final maturation of oocytes occur in response to stimulation by pituitaryderived luteinizing hormones (LH) that act on the somatic granulosa and theca cells surrounding the oocyte. Shortly after stimulation by the preovulatory surge of LH, oocytes arrested at the late prophase resume meiosis characterized by germinal vesicle (nuclear envelope) breakdown (GVBD), chromosome condensation, and extrusion of the first polar body in preparation for fertilization and early embryonic development. Recent studies demonstrated that the endocrine hormone LH stimulates ovarian production of EGF-like factors from granulosa cells and insulin-like 3 from theca cells to promote GVBD (1, 2). In addition to nuclear maturation exemplified by GVBD and extrusion of the first polar body to complete the first meiotic division, oocytes also undergo cytoplasmic maturation characterized by cytoplasmic changes essential for monospermic fertilization, processing of the sperm, and preparation for development to preimplantation embryos (3, 4). Although the spermatozoon provides an essential element for embryo generation, the developmental fate of the embryo is principally dictated by the oocyte. However, few studies have explored ovarian factors that may be important for the conditioning of the oocyte in preparation for fertilization and preimplantation development.Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of proteins known to activate the highaffinity TrkB receptor and the pan-neurotrophin low-affinity receptor p75 (5). Although neurotrophins are widely expressed in the central nervous syst...

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Section: Discussionsupporting

confidence: 93%

Ovarian brain-derived neurotrophic factor (BDNF) promotes the development of oocytes into preimplantation embryos

Kawamura

Mulders

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

168

126

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“…With BCB+ oocytes, the difference between the percentage of cleaved embryos (45.3%) and the percentage of 2PN oocytes (39.7%) was reduced which suggests a lower number of haploid embryos obtained with BCB+ oocytes matured with cysteamine. Several authors have found an effect of intracytoplasmic GSH levels on MPN formation [15,16,28,31,33,34]. In our study we observed significantly higher percentages of MPN in oocytes matured with cysteamine compared to the control group (80.3 vs. 61.5%, respectively).…”

Section: Discussionsupporting

confidence: 59%

“…In oocytes, intracytoplasmic GSH participates in the transformation of the fertilising sperm head in the male pronucleus. It is believed that at fertilisation, a high intracellular GSH content is needed for the transformation of the disulphide-stabilised sperm nucleus into the male pronucleus [30,31]. Indeed, the decrease in intracellular GSH of mouse oocytes impairs the decondensation of the sperm nucleus [32].…”

Section: Discussionmentioning

confidence: 99%

Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation

et al. 2003

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-The aim of this study was to assess the effect of oocyte selection using the brilliant cresyl blue (BCB) test plus the addition of cysteamine to the in vitro maturation (IVM) medium to improve the in vitro embryo development of prepubertal goat oocytes. The oocytes were exposed to 26 µM BCB and classified according to their cytoplasm coloration: BCB+ (oocytes with blue cytoplasm) and BCB-(unstained oocytes). The oocytes were matured in a conventional IVM medium supplemented with cysteamine 100 µM. The control group consisted of oocytes not exposed to BCB and matured without cysteamine. The IVM-oocytes were inseminated and cultured in synthetic oviductal fluid (SOF) for 7 days. The normal fertilisation rate (oocytes showing 2 pronuclei and 1 sperm tail) of BCB+ oocytes (40%) was higher than those of BCB-(21%) and control oocytes (22%). The percentage of morulae plus blastocysts was higher (P < 0.05) in the BCB+ group than in the BCB-group (23.8 vs. 5.1%, respectively). In conclusion, the integration of the BCB test and the addition of cysteamine in the protocol of in vitro embryo production from prepubertal goat oocytes has improved the developmental rates of embryo development. embryo / IVF / IVM / thiol

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“…Particularly interesting is the fact that the addition of CYS to IVM medium in pig oocytes results in increased glutathione (GSH, a derivative product of CYS) synthesis by the pig oocytes (Yoshida et al 1993), enhancing in vitro development after IVF (Grupen et al 1995;Kishida et al 2004). In addition, GSH synthesis during oocyte maturation is an important factor for promoting their ability to form a MPN after IVF (Yoshida et al 1993;Sawai et al 1997), and GSH is known to be a prerequisite to ensure sperm chromatin decondensation (Perreault et al 1984).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, GSH synthesis during oocyte maturation is an important factor for promoting their ability to form a MPN after IVF (Yoshida et al 1993;Sawai et al 1997), and GSH is known to be a prerequisite to ensure sperm chromatin decondensation (Perreault et al 1984). Accordingly, porcine IVM media have routinely been supplemented with CYS.…”

Section: Introductionmentioning

confidence: 99%

Male pronucleus formation after ICSI: effect of oocyte cysteine or sperm Triton X-100 treatments

García-Mengual¹,

Silvestre²,

Salvador³

et al. 2015

CZECH J ANIM SCI

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ABSTRACT:In pigs, intracytoplasmic sperm injection (ICSI) efficiency is still poor. The inadequate decondensation of the sperm chromatin, its transformation into the male pronucleus (MPN) together with the subsequent inability to activate the oocyte, seem to be the main causes of the low ICSI efficiency. In order to improve the MPN formation we took two different approaches. On the one hand, the in vitro culture (IVC) medium post-ICSI was supplemented with 1.71mM cysteine (CYS). Alternatively, the sperm membrane was digested with Triton X-100 (TX) before ICSI, to improve the exposure of the sperm chromatin to the oocyte cytoplasm. After 6 h post-ICSI, the activation rate was significantly higher in TX group (70.0%) compared with CYS and control groups (42.2% and 48.9%, respectively; P < 0.05). However, no significant differences between the three groups were observed in terms of the number of pronuclei, 2PN (oocytes with 2 pronuclei and no visible sperm), and 1PN + sperm (oocytes with 1 pronucleus and one sperm head). At 22 h post-ICSI, the activation rates were similar in TX, CYS, and control groups (73.1, 78.9, and 75.7%, respectively). In addition, we did not observe significant differences between TX, CYS, and control groups for the number of pronuclei, 2PN (52.6, 56.7, and 50%, respectively) or 1PN + sperm (21.1, 33.3, and 32.1%, respectively). While no cleavage was observed in the CYS group, no significant differences in the cleavage rate were observed between control (21.3%) and TX (10.5%) groups. In summary, and under our conditions, neither CYS supplement, nor sperm TX pre-treatment were able to improve MPN formation at 6 and 22 h post-ICSI. However, the sperm TX pre-treatment improved oocyte activation at 6 h post-ICSI, although 22 h post-ICSI such a beneficial effect did not persist.Keywords: porcine; assisted reproductive technology; pronuclear formation List of abbreviations: ICSI = intracytoplasmic sperm injection, PN = pronucleus, MPN = male pronucleus, IVC = in vitro culture, CYS = cysteine, TX = Triton X-100, IVP = in vitro production, IVM = in vitro maturation, IVF = in vitro fertilization, EGF = epidermal growth factor, GSH = glutathione, COC = cumulus oocyte complex, PZM-3 = porcine zygote medium-3, MM199 = maturation medium, HM199 = handling medium 242

show abstract

Glutathione Concentration during Maturation and after Fertilization in Pig Oocytes: Relevance to the Ability of Oocytes to Form Male Pronucleus1

Cited by 333 publications

References 32 publications

Ovarian brain-derived neurotrophic factor (BDNF) promotes the development of oocytes into preimplantation embryos

Ovarian brain-derived neurotrophic factor (BDNF) promotes the development of oocytes into preimplantation embryos

Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation

Male pronucleus formation after ICSI: effect of oocyte cysteine or sperm Triton X-100 treatments

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