Glutathione levels in hepatic and extrahepatic tissues of mice as a function of age

Stohs, Sidney J.; Hassing, Jeanne M.; Al‐Turk, Walid A.; Masoud, Asaad N.

doi:10.1007/bf02435000

Cited by 51 publications

(4 citation statements)

References 14 publications

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“…The GSH level in the 24-month-old mice was higher than in 9-month-old ani mals but was significant only when the level was expressed in terms of pig/106 cells. These results are in contrast with the results ob tained by Stohs et al [15] where the GSH level in whole blood of mice increased when comparing young and middle-aged animals but decreased in old mice. The reasons for these differences are not known; however, the results reported for whole blood GSH One unit is defined as the amount of enzyme cat alyzing the formation of I pmol product/min/106 cells.…”

Section: Resultscontrasting

confidence: 57%

“…As previously noted with GSH these re sults are in sharp contrast to the marked decrease in whole blood GST seen in 18-month-old female mice [ 15]. It has also been reported that GST activities in rat and rabbit liver, lung, testes, ovaries, adrenal glands and serum are low in neonatal and/or prenatal One unit of activity is defined as the amount of enzyme which will oxidize I pmol of NADPH/ min/IO6 cells.…”

Section: Resultscontrasting

confidence: 42%

“…GSH also plays an important role in cells as an antioxidant and, since free radicals may be responsible for cellular aging [13,14], attempts have been made to correlate GSH and its metabolizing enzymes to the aging process [15,16].…”

Section: Introductionmentioning

confidence: 99%

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Changes in Glutathione, Glutathione Reductase and Glutathione-S-Transferase as a Function of Cell Concentration and Age

et al. 1987

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The activities of glutathione-S-transferase (GST) and glutathione reductase (GSR) in mouse lymphocytes as a function of cell concentration and age were determined. Lymphocytes from 2-, 9- and 24-month-old mice were isolated and the activity of GST was determined spectrophotometrically using 1-chloro-2,4-dinitrobenzene as the substrate. Lymphocyte concentrations of 0.44, 0.75 and 0.55 × 10⁶ cells/ml were found to be optimal for assaying GST in 2-, 9- and 24-month-old mice, respectively. Determination of GSR activity was based on NADPH reduction of oxidized glutathione and cell concentrations of 0.29, 0.30 and 0.22 × 10⁶ cells/ml were chosen for assaying the enzyme activity for the three age groups, respectively. Glutathione levels and GSR activity of mice lymphocytes were higher in 2- and 24-month-old mice as compared to 9-month-old animals. However, GST activity of mouse lymphocytes was low in 2- and 9-month-old mice and increased significantly in 24-month-old animals.

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Section: Resultscontrasting

confidence: 57%

Section: Resultscontrasting

confidence: 42%

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Changes in Glutathione, Glutathione Reductase and Glutathione-S-Transferase as a Function of Cell Concentration and Age

et al. 1987

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“…The role of GSH in the aging process has been a subject of controversy for more than a decade. Some investigators observed a decline in the GSH content of tissues from old animals and proposed that diminished GSH concentrations were responsible, at least in part, for mammalian aging [Hazelton and Lang, 1980;Stohs et al, 1980;Laganiere and Yu, 19891. In contrast, others found that GSH levels were well maintained in old age and that declining GSH was not a general phenomenon [Rikans and Moore, 1988;Carrillo et al, 1989;Barja de Quiroga et al, 19901. Some of the contradictory findings about the effects of aging on GSH may be attributable to differences in species, sex, strain, nutritional status, or ages compared; others, however, cannot be reconciled on these grounds.…”

Section: Introductionmentioning

confidence: 99%

Diurnal variation in hepatic glutathione content as a function of age

Rikans

Nokubo

Kanai

et al. 1992

Drug Development Research

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Rikans, L.E., M. Nokubo, S. Kanai, and K. Kitani: Diurnal variation in hepatic glutathione content as a function of age. Drug Dev. Res. 26:461-465, 1992.The purpose of this study was to investigate the influence of aging on the diurnal variation in glutathione (GSH) content of mouse liver. Young (7 months) and old (27 months) C57BL mice were housed under standarized conditions with lighting from 0800 to 2000 and free access to food and water. GSH was determined in acid extracts of liver homogenates by HPLC separation and fluorometric detection of the o-phthaldialdehyde derivative. A diurnal rhythm in hepatic GSH concentration was evident in young and old mice of both sexes.Peak levels of GSH occurred at 1000 and were unaffected by age or gender: however, age and sex-dependent differences were observed in the lowest concentration of GSH and in the amplitude of the GSH cycle. Hepatic GSH levels at 2200 and 0600 were 30-35% lower in old males than in young males and 25-40% lower in young females than in young males. The results may provide an explanation for the inconsistencies in previous findings about the influence of aging on hepatic GSH concentrations. o 1992 Wiiey-Liss, inc.

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Interactions between Occupational and Environmental Factors in Toxicology, Hazard Evaluation and Risk Assessment

Gomes

Meek

2009

General, Applied and Systems Toxicology

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Occupational and environmental exposures to toxicants rarely occur in isolation or singly and the potential for multiple exposures occurring with different degrees of overlap and multiple numbers of toxicants is high. Interactions occur when multiple chemicals are present in biological systems at the same time. The nature and the intensity of the interactions vary greatly from individual to individual and are dependent upon a number of factors. Primarily, the interaction response is dependent both upon the chemicals in question and the individual that is exposed. Chemical‐associated factors include mechanisms or modes of action of the chemicals, timing of exposure and dose of exposure. Individual‐associated factors include personal characteristics, lifestyle factors, physiological factors and genetic factors. An integrated systems‐biology approach is necessary to evaluate the exposure, the interaction and the biological response. Newer technologies (omics) could play a significant role in assessing the risk of interaction from exposure to multiple toxins.

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Glutathione levels in hepatic and extrahepatic tissues of mice as a function of age

Cited by 51 publications

References 14 publications

Changes in Glutathione, Glutathione Reductase and Glutathione-S-Transferase as a Function of Cell Concentration and Age

Changes in Glutathione, Glutathione Reductase and Glutathione-S-Transferase as a Function of Cell Concentration and Age

Diurnal variation in hepatic glutathione content as a function of age

Interactions between Occupational and Environmental Factors in Toxicology, Hazard Evaluation and Risk Assessment

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