Glutathione Reductase in Leaves of Cowpea: Cloning of Two cDNAs, Expression and Enzymatic Activity under Progressive Drought Stress, Desiccation and Abscisic Acid Treatment

Contour-Ansel, Dominique; Torres-Franklin, Maria Lucia; Carvalho, Maria Helena Cruz de; d’Arcy-Lameta, Agnès; Zuily-Fodil, Yasmine

doi:10.1093/aob/mcl217

Cited by 127 publications

(57 citation statements)

References 33 publications

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“…ABA application significantly enhances the expression of GR genes in rice (Kaminaka et al 1998) and cowpea (Contour-Ansel et al 2006). In the present study, exogenous ABA enhanced the expression OsGR2 and OsGR3 in rice roots (Fig.…”

Section: Discussionmentioning

confidence: 99%

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NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid

et al. 2009

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Reactive oxygen species (ROS) play an important role in NaCl stress. Plants tolerant to NaCl stress may evolve certain strategies to remove these ROS, thus reducing their toxic effects. Therefore, the expression patterns of the gene family encoding glutathione reductase (GR, EC 1.6.4.2) were analyzed in roots of etiolated rice (Oryza sativa L.) seedlings in response to NaCl stress. Semi-quantitative RT-PCR was applied to quantify the mRNA levels for one cytosolic (OsGR2) and two chloroplastic (OsGR1 and OsGR3) isoforms of glutathione reductase identified in the rice genome. The expression of OsGR2 and OsGR3 but not OsGR1 was increased in rice roots treated with 150 mM NaCl. The Rab16A is an abscisic acid (ABA)-responsive rice gene. Increasing concentrations of ABA, from 1 to 12 μM, progressively increased the expression of OsRab16A in rice roots. In the present study, the ABA level was judged by the expression of OsRab16A in rice roots. Treatment with 150 mM NaCl induced the expression of OsRab16A, and the expression increased with increasing concentrations of ABA, which suggests that ABA may be involved in this response in rice roots. In fact, exogenous application of ABA enhanced the expression of OsGR2 and OsGR3 in rice roots. On inhibiting ABA accumulation with sodium tungstate (Tu), an inhibitor of ABA biosynthesis, the expression of OsGR2 and OsGR3 was still induced by NaCl; therefore, NaCl-triggered expression of OsGR2 and OsGR3 in rice roots is not mediated by accumulation of ABA. However, NaCl treatment could induce H 2 O 2 production in rice roots, and H 2 O 2 treatment resulted in enhanced OsGR2 and OsGR3 induction. On inhibiting the NaCl-induced accumulation of H 2 O 2 with diphenylene iodonium, the expression of OsGR2 and OsGR3 was also suppressed. Moreover, the increase in H 2 O 2 level was prior to the induction of OsGR2 and OsGR3 in NaCl-treated rice roots. Thus, H 2 O 2 , but not ABA, is involved in regulation of OsGR2 and OsGR3 expression in NaCltreated rice roots.

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Section: Discussionmentioning

confidence: 99%

“…The expression of GR is enhanced in plants by drought/desiccation (Contour-Ansel et al 2006;Torres-Franklin et al 2008) and salt (Kaminaka et al 1998). Abscisic acid (ABA) and H 2 O 2 accumulate in plants under salt stress (Moons et al 1995;Montero et al 1997;Tsai et al 2005;Hong et al 2007).…”

Section: Introductionmentioning

confidence: 99%

NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid

et al. 2009

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“…During abiotic stresses, upon reaction with oxidizing agents, GSH, a tripeptide composed of the amino acids glutamic acid, cystine and glycine (γ-L-Glutamyl-L-cystine glycine), is converted to GSSG [11]. It has been reported that glutathione levels (GSH/GSSG ratio) changes rapidly in response to water deficit, thus helping maintain the redox homeostasis of the cell [12]. In wheat (Triticum sp.)…”

Section: Effects Of 17β-estradiol Application On Stress-related Biochmentioning

confidence: 99%

“…A common consequence of various abiotic stresses, such as drought, salt, heavy metal and other stresses, is the production of reactive oxygen species (ROS) in plant cells and oxidative damage to tissues [10]. Studies have shown a direct correlation between the enhanced antioxidant activities and drought tolerance in various plant species [10] [11] [12]. Treatment of germinating bean (Phaseolus vulgaris) seeds with various concentrations of progesterone, β-estradiol and androsterone resulted in increased activity of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT) in plants grown under drought stress.…”

Section: Introductionmentioning

confidence: 99%

Alleviation of Drought Stress in <i>Arabidopsis thaliana</i> by 17<i>β</i>-Estradiol Application

Upadhyay¹,

Maier²

2016

AJPS

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Animal steroidal hormones, including estrogens, are being introduced into the agricultural soil and water supply from increased pharmaceutical and farm waste. Considering the current levels of xenoestrogen contamination of plant environments in view of the climate change induced drought conditions, this study was designed to understand the effect of estradiol (ES) application on Arabidopsis drought stress responses. Estradiol treatment (10 nM, 100 nM) of plants subjected to drought stress conditions by withholding water for 7 days resulted in increased tolerance to drought stress reflected in the significantly higher plant survival rates of 74% and 78%, respectively compared to control plants' survival rates of 36% (no treatment) and 40% (mock treatment). Estradiol application significantly increased the content of glutathione, proline and H 2 O 2 and significantly enhanced the transcription of the stress responsive genes GSTU3, GER5, HSP101, and HSP70b. A high concentration of ES (10 µM) did not protect plants against drought stress and proved to be toxic. These results provide new insight into the effect of ES on drought-stress responses in Arabidopsis with possible practical agricultural applications regarding the effect of environmental estrogens on crop plants.

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“…The effects of water stress reported in the literature on enzyme activities are disputable, depending both on the degree of tolerance of the plant, and on the way of water stress (Contour-Ansel et al 2006). In any case, these systems in overall, help plants growing in adverse environments alleviate the deleterious effect of active oxygen species (AOS) which are produced at increased rates (Jebara et al 2005).…”

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confidence: 99%

Drought-induced oxidative damage and antioxidant responses in peanut (Arachis hypogaea L.) seedlings

et al. 2010

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Two cultivars of peanut (Arachis hypogaea L.) which were designated as resistant (Florispan) and sensitive (Gazipasa) according to their growth retardation under drought stress conditions were compared for their oxidative damage and antioxidant responses. Sixteen days-old peanut seedlings were subjected to PEG-6000 solutions of two different osmotic potentials; -0.4 and -0.8 MPa, and various growth parameters, photosystem II activity, changes in malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 ) and proline levels, activities of ascorbate peroxidase (APX), catalase (CAT), peroxidase (POX) and gluthatione reductase (GR) enzymes were determined. Both cultivars exhibited water deficit at -0.8 MPa osmotic potential of PEG-6000 and H 2 O 2 levels significantly increased during exposure to -0.4 MPa osmotic potential. However, H 2 O 2 levels were under control in both cultivars at exposure to -0.8 MPa osmotic potential. Significant proline accumulation was observed in the tissues of cv. Florispan at -0.8 MPa osmotic potential, whereas proline accumulation did not appear to be an essential part of the protection mechanism against drought in cv. Gazipasa. No significant variation in chlorophyll fluorescence values were detected in neither of the cultivars. Enzyme activity measurements revealed that Gazipasa copes well with lesser magnitudes of drought stress by increasing the activity of mainly APX, and during harsh stress conditions, only APX maintains its activity in the tissues. In cultivar Florispan, GR activity appears to take role in lesser magnitudes of drought stress, whereas CAT and APX activities appear to be very crucial antioxidative defenses during intense stress conditions. The results indicate that, the level of proline and activities of the enzymes CAT and APX are important mechanisms for the maintenance of drought tolerance in peanut plants.

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Glutathione Reductase in Leaves of Cowpea: Cloning of Two cDNAs, Expression and Enzymatic Activity under Progressive Drought Stress, Desiccation and Abscisic Acid Treatment

Cited by 127 publications

References 33 publications

NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid

NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid

Alleviation of Drought Stress in <i>Arabidopsis thaliana</i> by 17<i>β</i>-Estradiol Application

Drought-induced oxidative damage and antioxidant responses in peanut (Arachis hypogaea L.) seedlings

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Glutathione Reductase in Leaves of Cowpea: Cloning of Two cDNAs, Expression and Enzymatic Activity under Progressive Drought Stress, Desiccation and Abscisic Acid Treatment

Cited by 127 publications

References 33 publications

NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid

NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid

Alleviation of Drought Stress in &lt;i&gt;Arabidopsis thaliana&lt;/i&gt; by 17&lt;i&gt;β&lt;/i&gt;-Estradiol Application

Drought-induced oxidative damage and antioxidant responses in peanut (Arachis hypogaea L.) seedlings

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Alleviation of Drought Stress in <i>Arabidopsis thaliana</i> by 17<i>β</i>-Estradiol Application