Glutathione S-transferase activities in phytophagous insects: Induction and inhibition by plant phototoxins and phenols

Lee, Keywan

doi:10.1016/0020-1790(91)90001-u

Cited by 109 publications

(50 citation statements)

References 31 publications

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“…The authors reported significantly higher GST activity in field-collected C. gibbosum feeding on gorgonians Gorgonia ventalina and Briareum asbestinum, suggesting that GST expression varies in response to different suites of gorgonian allelochemicals. Additionally, GST activity from C. gibbosum cytosolic preparations was among the highest ever reported from a molluscan digestive gland and was similar to values described from Papilio polyxenes, a specialist insect that feeds solely on chemically defended plants [10,11]. In a subsequent study [12], thin-layer chromatographic profiles of nonpolar tissue extracts from C. gibbosum feeding on G. ventalina did not mirror those of its octocoral prey, lending further support to the idea that this gastropod predator has the capacity to biotransform dietary compounds to readily excretable metabolites.…”

Section: Introductionsupporting

confidence: 75%

Proteomic identification, cDNA cloning and enzymatic activity of glutathione S-transferases from the generalist marine gastropod, Cyphoma gibbosum

Whalen¹,

Morin²,

Lin³

et al. 2008

Archives of Biochemistry and Biophysics

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Short title: Characterization of glutathione S-transferases from Cyphoma gibbosumFootnote: The nucleotide and translated amino acid sequences for C. gibbosum GSTs have been deposited in GenBank with the following accession nos. EU008563 (CgGSTM1) and EU008562 (CgGSTM2).Abbreviations footnote: CDNB, 1-chloro-2,4-dinitrobenzene; GST, glutathione S-transferase; nrDB, non-redundant database; RACE, rapid amplification of cDNA ends; RT-PCR, reverse transcriptase polymerase chain reaction 2 ABSTRACT Glutathione S-transferases (GST) were characterized from the digestive gland of Cyphoma gibbosum (Mollusca; Gastropoda), to investigate the possible role of these detoxification enzymes in conferring resistance to allelochemicals present in its gorgonian coral diet. We identified the collection of expressed cytosolic Cyphoma GST classes using a proteomic approach involving affinity chromatography, HPLC and nanospray liquid chromatography-tandem mass spectrometry (LC-MS/MS). Two major GST subunits were identified as putative mu-class GSTs; while one minor GST subunit was identified as a putative theta-class GST, apparently the first theta-class GST identified from a mollusc. Two Cyphoma GST cDNAs (CgGSTM1 and CgGSTM2) were isolated by RT-PCR using primers derived from peptide sequences. Phylogenetic analyses established both cDNAs as mu-class GSTs and revealed a mollusc-specific subclass of the GST-mu clade. These results provide new insights into metazoan GST diversity and the biochemical mechanisms used by marine organisms to cope with their chemically defended prey.

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Section: Introductionsupporting

confidence: 75%

Proteomic identification, cDNA cloning and enzymatic activity of glutathione S-transferases from the generalist marine gastropod, Cyphoma gibbosum

Whalen¹,

Morin²,

Lin³

et al. 2008

Archives of Biochemistry and Biophysics

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“…The high GST levels reported in Cyphoma gibbosum (2.76 to 4.05 pm01 min-'mg-') have also been reported in Papilio polyxenes, a specialist insect herbivore whose larvae feed on toxic, furanocoumarincontaining plants (Lee 1991). High GST activity in other molluscs has also been correlated with feeding on allelochemically defended macroalgae , Schlenk & Buhler 1988.…”

Section: Glutathione Transferases Are a Group Of Phase I1mentioning

confidence: 83%

“…The model substrate CDNB is used in many studies of GST and provides an indication of the combined catalytic activity of all GST isozymes (Clark 1989). In addition to conjugating reactive metabolites formed by P450 oxidative metabolism, GSTs are also induced by dietary allelochemicals in herbivorous insects (Yu 1982, Wadleigh and Yu 1987, Lee 1991. Wadleigh & Yu (1987 have further demonstrated metabolism and detoxification of a$-unsaturated carbonyl allelochemicals.…”

Section: Glutathione Transferases Are a Group Of Phase I1mentioning

confidence: 99%

Biotransformation enzymes in Cyphoma gibbosum (Gastropoda: Ovulidae)- implications for detoxification of gorgonian allelochemicals

Vrolijk¹,

Targett²

1992

Mar. Ecol. Prog. Ser.

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The role of biotransformation enzymes in metabolism of lipophilic, dietary allelochemicals such as terpenoids and steroids has not been studied in marine organisms despite the significance of allelochemicals in the evolution of biotransformation enzymes and the importance of these enzymes in detoxification of anthropogenic xenobiotics. Here we report on cytochrome P450 and glutathione transferase (GST) from the digestive gland of Cyphoma gibbosum, a specialist predator that feeds on allelochemically rich gorgonian corals. Levels of these enzymes were compared from C. gibbosum collected in Belize from 4 different gorgonian prey species (Briareum asbestinum, Gorgonia ventalina, Plexaura homomalla, and Pseudopterogorgia americana). Cytochrome P450 specific content was very low and only quantifiable in C, gibbosum collected from P americana (21.4 to 27.4 pm01 mg-'1. A peak at ca 420 nm was also detected in most samples, indicative of degraded P450 or an independent, functional haemoprotein Methoxyresorufin 0-deethylase (MROD) and ethoxyresorufin 0-deethylase (EROD) were not detected in C. gibbosum. However, NADPH-cytochrome c (P4501 reductase (NCR) was detected in all samples and the specific activity ranged from 23.2 to 67.3 nmol min-' mg-' protein. Significantly higher NCR activity was detected in C. gibbosum collected from prostaglandin-rich P. homomalla. Glutathione transferase specific activity ranged from 2.76 to 4.05 pm01 min-' mg-l, ranking it among the highest ever reported (using l-chloro-2,4-dinitrobenzene as a model substrate). Significantly higher GST activity was detected in C. gibbosum collected from G. ventalina and B. asbestinum, suggesting that GST actlvity varies in response to different suites of gorgonian allelochemicals.

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“…Harmine and dietary methoxsalen increased glutathione transferase activity toward the cytosolic fraction of midgut almost two folds in Trichoplusia ni [28] . The effects of harmine on the food utilization efficiencies and enzymatic detoxification systems were investigated in the polyphagous noctuid Trichoplusia ni.…”

Section: Effect Of Harmine On Enzyme Systemsmentioning

confidence: 94%

A review on medicinal importance, pharmacological activity and bioanalytical aspects of beta-carboline alkaloid “Harmine”

Patel

Gadewar

Tripathi

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

219

120

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Glutathione S-transferase activities in phytophagous insects: Induction and inhibition by plant phototoxins and phenols

Cited by 109 publications

References 31 publications

Proteomic identification, cDNA cloning and enzymatic activity of glutathione S-transferases from the generalist marine gastropod, Cyphoma gibbosum

Proteomic identification, cDNA cloning and enzymatic activity of glutathione S-transferases from the generalist marine gastropod, Cyphoma gibbosum

Biotransformation enzymes in Cyphoma gibbosum (Gastropoda: Ovulidae)- implications for detoxification of gorgonian allelochemicals

A review on medicinal importance, pharmacological activity and bioanalytical aspects of beta-carboline alkaloid “Harmine”

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