Glutathione S-Transferase Gene Polymorphisms and the Development of New-Onset Diabetes After Liver Transplant

Abstract

Objectives: The association between the glutathione Stransferase polymorphisms and the development of new-onset diabetes mellitus after liver transplant was studied. Materials and Methods: Peripheral blood samples were collected from 106 liver transplant patients divided into 2 groups: 52 with new-onset diabetes mellitus and 54 without new-onset diabetes mellitus; 169 healthy individuals with no clinical evidence of diabetes mellitus were selected as a control group. The multiplex polymerase chain reaction technique was used for genotyping GSTM1 and GSTT1 genes, using the cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1) gene as an internal control. The genotype of GSTP1 was determined using the restriction fragment length polymorphism-polymerase chain reaction technique. Results: The frequency of both GSTM1 null and GSTT1 null genotypes was not significantly different in liver transplant patients with new-onset diabetes mellitus compared with the control group (P = .11 for GSTM1; P = .71 for GSTT1). Also, there was no statistically significant association between the frequency of the GSTP1 genotypes in the liver transplant patients with new-onset diabetes mellitus compared with controls. Neither GSTM1 nor GSTT1 null genotypes were associated with the risk of developing new-onset diabetes mellitus (P = .22 for GSTM1; P = .56 for GSTT1). However, the frequency of the heterozygous mutation (AG) in the A313G GSTP1 polymorphism in patients with new-onset diabetes mellitus was significantly higher than in patients without new-onset diabetes mellitus (55.8% vs 7.4%; P = .00). Thus, the risk of developing new-onset diabetes mellitus was significantly higher in patients presenting with heterozygous GSTP1 genotypes (odds ratio = 15.76; 95% confidence interval = 4.53-60.28; P = .00). Conclusions: The GSTP1 AG genotype was associated with an increased susceptibility to the development of new-onset diabetes mellitus after liver transplant.

Glutathione S-transferases gene polymorphism influence on the age of diabetes type 2 onset

“…16 Another study, conducted in Iran, revealed that GSTP1Ile105Val polymorphism is associated with an increased risk of newonset diabetes mellitus after liver transplantation. 10 On the other hand, a meta-analysis of 18 studies has shown no significant association between GSTP1 polymorphism and the risk of T2D. 19 Results of our study has shown only slight differences in GST gene polymorphism frequency between patients with T2D and healthy controls, statistically not significant (table 2).…”

Single-nucleotide polymorphisms as important risk factors of diabetes among Middle East population

“…The heterozygous (AG) genotype was more frequent in liver transplant cases with NODM compared with non-NODM cases. AG allele of the GSTP1 (A313G) increased risk of NODM in a subpopulation of Iranian cases [ 163 ]. A study evaluated the genotype frequencies of the GSTP1, GSTT1, and GSTM1 polymorphisms in order to find the probable correlation of the GST polymorphisms with susceptibility to DM among the Turkish individuals.…”