Glutathione S-transferase in the developmental stages of the insect Apis mellifera macedonica

Papadopoulos, Andrew; Polemitou, Irene; Laifi, Pshychoula; Yiangou, Astero; Tananaki, Chrysoula

doi:10.1016/j.cca.2004.09.009

Cited by 14 publications

(16 citation statements)

References 20 publications

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“…Our study showed a gradual increase in the activity of GST (from 0.739 to 1.006 U•mg -1 ) with increasing doses of oxamyl in comparison with the control (0.724 U•mg -1 ). An increased specific activity of GST was observed in honeybees exposed to permetrin, deltametrin and flumetrin [20]. The GST of the class sigma showed high affinity to products of lipid peroxidation.…”

Section: Discussionmentioning

confidence: 95%

Antioxidant Enzymes of Honeybee Larvae Exposed to Oxamyl

2019

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Oxamyl is a carbamate insecticide used to control a broad spectrum of insects. It can also affect non-targeted organisms when applied incorrectly. The world food production depends partially on honeybee pollination abilities and therefore it is directly linked to the health of bees. The success of the colony development depends, among other factors, on the health of the larvae. The first 6 days are crucial for their development. In this stage, the worker larvae grow exponentially and may be exposed to xenobiotics via their diet. In this study, we investigated the effect of oxamyl on honeybee larvae (Apis mellifera) by monitoring the changes in their antioxidant enzyme system. The activities of superoxide dismutase, catalase and glutathione-S-transferase were determined in the homogenates of in vitro reared honeybee larvae after their single dietary exposure to oxamyl at doses of 1.25, 2.5, 5, 10 and 20 µg a.i./larva (a. i.—active ingredient). The doses of oxamyl did not cause statistically significant changes in the activities of the enzymes. Even a slight activation of these enzymes protected the larvae from the adverse effects of the reactive oxygen species (ROS). Marked changes in both the enzyme activity and the content of lipid peroxidation products were observed at the oxamyl dose of 10 µg a. i./larva. This fact may indicate a potential oxidative damage to the larvae. These results allowed us to assume that the toxic effects of oxamyl involves not only the inhibition of acetylcholine esterase but is also associated with ROS production.

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Section: Discussionmentioning

confidence: 95%

Antioxidant Enzymes of Honeybee Larvae Exposed to Oxamyl

2019

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“…In honeybees, there are qualitative and quantitative changes in the GST isoforms during the course of development. In adults, the alkaline isoform is the most prevalent (>84%) . Papadopoulos et al observed that in adult bees, the acid and alkaline isoforms of GST show differential responses to deltamethrin and methyl parathion.…”

Section: Discussionmentioning

confidence: 99%

“…In adults, the alkaline isoform is the most prevalent (>84%) . Papadopoulos et al observed that in adult bees, the acid and alkaline isoforms of GST show differential responses to deltamethrin and methyl parathion. Thus, the small variation found in GST activity after exposure of honeybees to fipronil may be explained by the variability between the isoforms of GST, as well as by the insecticide and the dose, showing that future studies using GST as a biomarker should measure both isoforms.…”

Section: Discussionmentioning

confidence: 99%

Biochemical response of the Africanized honeybee exposed to fipronil

Roat

Carvalho

Palma

et al. 2017

Enviro Toxic and Chemistry

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Bees are recognized worldwide for their social, economic, and environmental value. In recent decades they have been seriously threatened by diseases and high levels of pesticide use. The susceptibility of bees to insecticides makes them an important terrestrial model for assessing environmental quality, and various biomarkers have been developed for such assessments. The present study aimed to evaluate the activity of the enzymes acetylcholinesterase (AChE), carboxylesterase (CaE), and glutathione-S-transferase (GST) in Africanized honeybees exposed to fipronil. The results showed that fipronil at a sublethal dose (0.01 ng/bee) modulates the activity of CaE in all isoforms analyzed (CaE-1, CaE-2, and CaE-3) in both newly emerged and aged bees, and does not affect the activity of AChE or GST. The recovery of the bees after fipronil exposure was also investigated, and these results demonstrated that even the cessation of fipronil ingestion might not lead to complete recovery of individual bees. Even at low doses, fipronil was shown to cause changes in the activity of key enzymes in bees. The possible consequences of these changes are discussed. Environ Toxicol Chem 2017;36:1652-1660. © 2016 SETAC.

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“…It was thought that there were two ways to explain this observation. One assumed that some alterations to the protein structure occurred during the developmental stages to meet the continuously changing demands of the development of insects, and the differences in expression level of genes encoding specific enzymes might be another factor resulting in changes 37. As no significant difference in expression of AChE was found among larvae, pupae and adults of B. dorsalis , the changes in activities of AChE during the developmental stages could be caused by the nutritional condition, as the adults were reared on an artificial diet while the larvae were fed on banana.…”

Section: Discussionmentioning

confidence: 99%