Glutathione S-transferase in the insect Apis mellifera macedonica

Papadopoulos, Andrew; Polemitou, Irene; Laifi, Pshychoula; Yiangou, Astero; Tananaki, Chrysoula

doi:10.1016/j.cca.2004.09.010

Cited by 17 publications

(2 citation statements)

References 21 publications

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“…Several studies have demonstrated the involvement of the P450-, GST- or COE-enzyme families in pesticide and secondary metabolite detoxification in honey bees 13 14 15 16 17 18 . Mao et al (2009) were the first to assign a function to a specific honey bee cytochrome P450 enzyme by demonstrating that CYP6AS3 is involved in the detoxification of quercetin, a flavonol present in pollen and numerous floral nectars, while CYP9Q1, CYP9Q2 and CYP9Q3 were the first specific P450s identified to contribute to pesticide (tau-fluvalinate and coumaphos) detoxification in honey bees 17 .…”

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confidence: 99%

Detoxification mechanisms of honey bees (Apis mellifera) resulting in tolerance of dietary nicotine

Rand

Smit

Beukes

et al. 2015

Sci Rep

166

117

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Insecticides are thought to be among the major factors contributing to current declines in bee populations. However, detoxification mechanisms in healthy, unstressed honey bees are poorly characterised. Alkaloids are naturally encountered in pollen and nectar, and we used nicotine as a model compound to identify the mechanisms involved in detoxification processes in honey bees. Nicotine and neonicotinoids have similar modes of action in insects. Our metabolomic and proteomic analyses show active detoxification of nicotine in bees, associated with increased energetic investment and also antioxidant and heat shock responses. The increased energetic investment is significant in view of the interactions of pesticides with diseases such as Nosema spp which cause energetic stress and possible malnutrition. Understanding how healthy honey bees process dietary toxins under unstressed conditions will help clarify how pesticides, alone or in synergy with other stress factors, lead to declines in bee vitality.

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confidence: 99%

Detoxification mechanisms of honey bees (Apis mellifera) resulting in tolerance of dietary nicotine

Rand

Smit

Beukes

et al. 2015

Sci Rep

166

117

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“…While, both forms of chlorntraniliprole (technical-grade and formulation product) had a toxic effect after 4 or 72 h post-treatments (Williams et al 2020 ). On the other hand, few studies have demonstrated the detoxification enzymes to conventional insecticides in honeybees (Papadopoulos et al 2004 ; Johnson et al 2006 ).…”

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confidence: 99%

Sulfoxaflor influences the biochemical and histological changes on honeybees (Apis mellifera L.)

et al. 2023

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Pesticide application can have an adverse effect on pollinator honey bees, Apis mellifera L., ranging from mortality to sublethal effects. Therefore, it is necessary to understand any potential effects of pesticides. The present study reports the acute toxicity and adverse effects of sulfoxaflor insecticide on the biochemical activity and histological changes on A. mellifera. The results showed that after 48 h post-treatment, the LD25 and LD50 values were 0.078 and 0.162 µg/bee, respectively, of sulfoxaflor on A. mellifera. The detoxification enzyme activity shows an increase of glutathione-S-transferase (GST) enzyme on A. mellifera in response to sulfoxaflor at LD50 value. Conversely, no significant differences were found in mixed-function oxidation (MFO) activity. In addition, after 4 h of sulfoxaflor exposure, the brains of treated bees showed nuclear pyknosis and degeneration in some cells, which evolved to mushroom shaped tissue losses, mainly neurons replaced by vacuoles after 48 h. There was a slight effect on secretory vesicles in the hypopharyngeal gland after 4 h of exposure. After 48 h, the vacuolar cytoplasm and basophilic pyknotic nuclei were lost in the atrophied acini. After exposure to sulfoxaflor, the midgut of A. mellifera workers showed histological changes in epithelial cells. These findings of the present study showed that sulfoxaflor could have an adverse effect on A. mellifera.

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Identification and characterisation of ten glutathione S‐transferase genes from oriental migratory locust, Locusta migratoria manilensis (Meyen)

Qin

Jia

Liu

et al. 2011

Pest Management Science

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Glutathione S-transferase in the insect Apis mellifera macedonica

Cited by 17 publications

References 21 publications

Detoxification mechanisms of honey bees (Apis mellifera) resulting in tolerance of dietary nicotine

Detoxification mechanisms of honey bees (Apis mellifera) resulting in tolerance of dietary nicotine

Sulfoxaflor influences the biochemical and histological changes on honeybees (Apis mellifera L.)

Identification and characterisation of ten glutathione S‐transferase genes from oriental migratory locust, Locusta migratoria manilensis (Meyen)

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