Glutathione-S-transferase-pi (GST-pi) expression in renal cell carcinoma

Kaprilian, Christina; Horti, Maria; Kandilaris, Kosmas; Skolarikos, Andreas; Τράκας, Νικόλαος; Kastriotis, Ioannis; Deliveliotis, Charalambos

doi:10.15586/jkcvhl.2015.22

Cited by 4 publications

(5 citation statements)

References 17 publications

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“…Some studies indicated an overexpression of GSTP1 gene in esophageal cancer (Joshi et al., ), colorectal cancer (Zhang et al., ), renal cancer (Kaprilian et al., ), lung cancer (Yang et al., ), and in BC (Chen et al., ; Pljesa‐Ercegovac et al., ; Savic‐Radojevic et al., ). Other studies showed reduced GSTP1 gene expression in prostate, endometrial, hepatocellular, and ovarian cancers (Chan et al., ; Li et al., ; Lin et al., ; Martignano et al., ; Mian et al., ; Shilpa et al., ; Zelic et al., ; Zhang et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…In response to oxidative stress, a wide variety of stressed tumor cells show increased levels of GSTP1; GSTP1 overexpression was found in many tumors such as esophageal cancer (Joshi et al, 2005), colorectal cancer (Zhang et al, 2014), renal cancer (Kaprilian et al, 2015), lung cancer (Yang, Ebbert, Sun, & Weinshilboum, 2006), and breast cancer (Batist et al, 1986;Huang, Tan, Thiyagarajan, & Bay, 2003;Muftin, AL-Rubaiꞌe, Yaseen, & Aziz, 2015;Vecanova et al, 2011). These studies have also demonstrated that high levels of GSTP1 correlated with cancer drugs resistance, failure of chemotherapy, and poor prognosis of tumors.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of glutathione S‐transferase pi 1 expression and gene promoter methylation in Moroccan patients with urothelial bladder cancer

Hadami

Dakka

Bensaid

et al. 2018

Molec Gen & Gen Med

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BackgroundGlutathione S‐transferase pi 1 (GSTP1) is a cytosolic detoxifying enzyme that protects cells against deleterious effects of oxidative stress. Deregulated expression of GSTP1 protein and aberrant promoter methylation of GSTP1 gene were reported in various human tumors and were shown to be involved in the molecular pathway for cancer development.Aims and methodsIn this study, we aimed to determine the expression status of GSTP1 in relation to its gene promoter methylation in Moroccan population of 30 bladder cancer (BC) patients and in two noncancerous bladder tissues used as controls. GSTP1 expression was assessed by immunohistochemistry and GSTP1 gene promoter methylation status was studied by methylation‐specific PCR (MS‐PCR).ResultsGlutathione S‐transferase pi 1 was expressed in the two normal tissues. In BC cases, GSTP1 expression was strong in 23.33% (7/30), moderate in 60% (18/30), and weak in 13.33% (4/30) of cases, while GSTP1 was not expressed in one cancer case (3.33%). Variability of GSTP1 expression does not correlate with high‐grade cancer or invasive‐stage (p > 0.05). No GSTP1 gene promoter methylation was detected in all control and cancer cases.ConclusionOur data suggest that GSTP1 expression is not associated with BC development, limiting its use as a biomarker for BC management in Morocco. Moreover, difference in GSTP1 expression among BC cases is not due to GSTP1 promoter methylation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of glutathione S‐transferase pi 1 expression and gene promoter methylation in Moroccan patients with urothelial bladder cancer

Hadami

Dakka

Bensaid

et al. 2018

Molec Gen & Gen Med

View full text Add to dashboard Cite

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“…This variability is even more potentiated in cancer cells [29]. In general, GSTP1 over-expression seems to be a hallmark of proliferating cells in many solid tumors, including transitional cell carcinoma of urinary bladder [30,31], renal cell carcinoma [32,33], ovarian cancer [34,35], breast cancer [36,37] and colorectal cancer [38,39]. Regarding other GST classes, increased expression of GSTA1 is confirmed in colorectal cancer [39], GSTO1-1 is upregulated in transitional cell carcinoma [40], esophageal squamous cell carcinoma [41], pancreatic cancer [42], and breast cancer [43], while GSTM1 overexpression is observed in transitional cell carcinoma of urinary bladder [31], renal cell carcinoma [33] and breast cancer [44].…”

Section: Glutathione Transferases In Cancermentioning

confidence: 99%

Glutathione Transferases: Potential Targets to Overcome Chemoresistance in Solid Tumors

Plješa-Ercegovac

Savić-Radojević

Matić

et al. 2018

IJMS

110

101

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Multifunctional enzymes glutathione transferases (GSTs) are involved in the development of chemoresistance, thus representing a promising target for a novel approach in cancer treatment. This superfamily of polymorphic enzymes exhibits extraordinary substrate promiscuity responsible for detoxification of numerous conventional chemotherapeutics, at the same time regulating signaling pathways involved in cell proliferation and apoptosis. In addition to upregulated GST expression, different cancer cell types have a unique GST signature, enabling targeted selectivity for isoenzyme specific inhibitors and pro-drugs. As a result of extensive research, certain GST inhibitors are already tested in clinical trials. Catalytic properties of GST isoenzymes are also exploited in bio-activation of specific pro-drugs, enabling their targeted accumulation in cancer cells with upregulated expression of the appropriate GST isoenzyme. Moreover, the latest approach to increase specificity in treatment of solid tumors is development of GST pro-drugs that are derivatives of conventional anti-cancer drugs. A future perspective is based on the design of new drugs, which would selectively target GST overexpressing cancers more prone to developing chemoresistance, while decreasing side effects in off-target cells.

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“…Many molecules in the GST family play an important role in the treatment and prognosis of tumors. For example, GSTP1 overexpression is a marker of cell proliferation in a variety of tumors, such as transitional cell carcinoma of the bladder [ 22 , 23 ], renal epithelial renal cell carcinoma [ 24 , 25 ], ovarian cancer [ 26 , 27 ], breast cancer [ 28 , 29 ], and colorectal cancer [ 30 , 31 ]. GSTM1 and GSTM2 could serve as potential biomarkers of COAD prognosis [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

The Upregulation of GSTO2 is Associated with Colon Cancer Progression and a Poor Prognosis

Peng

Zhu

Liu

et al. 2023

Journal of Oncology

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Colorectal cancer is the second-leading cause of cancer-related mortality in the United States. Glutathione S-transferase can affect the development of cancer. Glutathione S-transferase omega 2, a member of the GST family, plays an important role in many tumors. However, the role of Glutathione S-transferase omega 2 in the development of colon cancer remains unclear. Herein, our study aimed to investigate the exact role of Glutathione S-transferase omega 2 in colon cancer. We used RNA sequencing data from The Cancer Genome Atlas and the Genotype-Tissue Expression database to analyze Glutathione S-transferase omega 2 expressions. Then, we explore the protein information of Glutathione S-transferase omega 2 in the Human Protein Atlas, GeneCards, and String database. In addition, western blot and immunohistochemistry were performed to evaluate the protein levels of Glutathione S-transferase omega 2 in colon cancer tissues. We acquire data from the Gene Expression Omnibus and The Cancer Genome Atlas databases. Also, we performed relevant prognostic analyses of these data. In addition, we performed a statistical analysis of the clinical data from The Cancer Genome Atlas database and the expression level of Glutathione S-transferase omega 2. Then, we performed Cox regression analysis and found independent risk factors for prognosis in patients with colon cancer. The Kyoto Encyclopedia of Genes and Genomes and Gene Ontology enrichment analyses were used to explore the potential biological functions of Glutathione S-transferase omega 2. The infiltration of colon cancer-immune cells was evaluated by the CIBERSORT method. RNA silencing was performed using siRNA constructs in HCT-116 and HT-29 cell lines. Cell Counting Kit-8 and EdU assays were performed to determine cell proliferation. Transwell experiments and scratch tests were used to determine cell migration. As for the mRNA and protein expression levels of cells, we used quantitative real-time PCR and western blot to detect them. Our research shows that Glutathione S-transferase omega 2 is overexpressed in colon cancer patients, and this overexpression is associated with a poor prognosis. The high expression of Glutathione S-transferase omega 2 is significantly correlated stage with stage, M, and N classification progression in colon cancer by statistical analysis. Univariate and multivariate Cox regression analyses showed that Glutathione S-transferase omega 2 was an independent risk factor for poor prognosis in colon cancer. In addition, we also found that Glutathione S-transferase omega 2 expression levels can affect the immune microenvironment of colon cancer cells. Gene silencing of Glutathione S-transferase omega 2 in HT-29 and HCT-116 cells significantly inhibited tumor growth and migration. In summary, we found that Glutathione S-transferase omega 2 can be used as a molecular indicator of colon cancer prognosis. In vitro, gene silencing of Glutathione S-transferase omega 2 inhibited colon cancer cells’ growth and migration.

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Glutathione-S-transferase-pi (GST-pi) expression in renal cell carcinoma

Cited by 4 publications

References 17 publications

Evaluation of glutathione S‐transferase pi 1 expression and gene promoter methylation in Moroccan patients with urothelial bladder cancer

Evaluation of glutathione S‐transferase pi 1 expression and gene promoter methylation in Moroccan patients with urothelial bladder cancer

Glutathione Transferases: Potential Targets to Overcome Chemoresistance in Solid Tumors

The Upregulation of GSTO2 is Associated with Colon Cancer Progression and a Poor Prognosis

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