Glutathione-S-transferase µ and theta gene polymorphisms as new risk factors of atopic bronchial asthma

Abstract

The genetic polymorphism of glutathione- S-transferase M1 (GSTM1) and glutathione- S-transferase T1 (GSTT1) genes and the cytochrome P4501A1 gene responsible for xenobiotic conjugating enzymes of the phase II and phase I detoxification system were studied by PCR-RFLP in the blood spots of 109 patients with atopic bronchial asthma and 90 healthy individuals. GSTM1 gene deletion (GSTM1(0/0)) was detected in 47.8% of individuals in the control group and in 76.1% of asthmatic patients. Individuals without the GSTM1 gene were at approximately 3.5--fold higher risk of developing asthma. The proportion of GSTT1(0/0) genotypes was significantly higher in the group of asthmatics (67.0%) than in controls (23.3%). The proportion of individuals with a deficiency in both GSTM1 and GSTT1 gene activity was more than four times higher in asthmatic patients than in the control group (54.1% and 12.2%, respectively). The frequency of the Ile-Val polymorphism of the CYP1A1 gene was similar in controls and asthmatic patients. This study shows the association of atopic bronchial asthma with GSTM1(0/0), GSTT1(0/0) genotypes.

The influence of genetic variation in oxidative stress genes on human noise susceptibility

“…These include polymorphic large deletions causing inactivation of two antioxidant genes, GSTM1 and GSTT1, that have previously been associated with several conditions where oxidative stress has been implicated. These include various forms of cancer (Strange et al, 2001), multiple sclerosis (Mann et al, 2000), ParkinsonÕs disease (Tan et al, 2000), asthma (Ivaschenko et al, 2002), and liver cirrhosis (Ghobadloo et al, 2004). For each of 5 other antioxidant genes, where no inactivating polymorphisms were present, several single nucleotide polymorphisms were used.…”

Implications of gene copy-number variation in health and diseases

“…49,50 CNVs of the GST genes were examined in patients with atopic asthma and it was found that the null genotypes of GSTT1 and GSTM1 together with GSTP1 Val/Val polymorphism have a significant role in the asthma pathogenesis. 51 CNV AND CARDIOVASCULAR DISEASE Structural variations are also found to be affecting the susceptibility to many cardiovascular diseases.…”

Impact of glutathione-S-transferase gene polymorphisms on enzyme activity, lung function and bronchial asthma susceptibility in Egyptian children

“…Asthma should be regarded as a multifactorial disease, involving both a genetic predisposition and environmental factors (Ivaschenko et al, 2002). The presence of inflammation in the airway is an important biochemical feature of asthma.…”