Glutathione S-transferases from larval Manduca sexta midgut: Sequence of two cdnas and enzyme induction

Comparative Studies of Substrate and Inhibitor Specificity of Glutathione S-Transferases in Six Tissues of Oxya chinensis (Thunberg) (Orthoptera: Acrididae)

“…Furthermore, conjugating-DCNB activity was not detected in any of these isozymes (Yu 2002). Multiple forms of GST were also observed in other insects, including Diptera (Snyder et al 1995), Coleoptera (Kostaropoulos et al 1996), Hymenoptera (Valles et al 2003), and Lepidoptera (Kirby and Ottea 1995). From present studies it can be inferred that the changes of substrate specificity, kinetic parameters and inhibitory properties of GST might be because of the presence of different and multiple isozymes among the six tissues of O. chinensis.…”

“…This suggested chinensis. Some studies showed that GST activities were the highest in the fat body and midgut (Snyder et al 1995;Tang et al 2005;Tate et al 1982). Furthermore, the relatively high GST activities in the hindgut might be related with the Malpighian tubes in the hindgut where the highest GST activity was found in other studies (Konno and Shishido 1992).…”

Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution

“…Therefore, information regarding the tissue distribution of specific GST isoforms is required in order to study the implications of GST activity in the detoxification of harmful physiological and xenobiotic electrophilic compounds. In this study, BmGSTD2 and BmGSTS2 showed high protein sequence identities to Delta-class and Sigma-class GSTs from the midgut of larvae of M. sexta (Snyder et al, 1995). In addition, BmGSTD2 had greater homology to Delta-class GST from the midgut of larvae of M. sexta (Snyder et al, 1995) than Delta-class GST from the fat body of B. mori (Yamamoto et al, 2005).…”

“…In this study, BmGSTD2 and BmGSTS2 showed high protein sequence identities to Delta-class and Sigma-class GSTs from the midgut of larvae of M. sexta (Snyder et al, 1995). In addition, BmGSTD2 had greater homology to Delta-class GST from the midgut of larvae of M. sexta (Snyder et al, 1995) than Delta-class GST from the fat body of B. mori (Yamamoto et al, 2005). Taken together, our results indicate that BmGSTD2 and BmGSTS2 are expressed exclusively in the gut of larvae and suggest that the expression of BmGSTD2 and BmGSTS2 is regulated in a tissuespecific manner.…”

“…An increase in the activity of these enzymes is associated with resistance to all of the major classes of insecticides (Fournier et al, 1992;Prapanthadara et al, 1993;Huang et al, 1998;Ranson et al, 2001;Vontas et al, 2001;Ortelli et al, 2003;Enayati et al, 2005;Lumjuan et al, 2005). Due to the important role of GST, GST genes have been cloned from several insect species (Snyder et al, 1995;Arruda et al, 1997;Ranson et al, 1997;Ding et al, 2003;Valles et al, 2003;Claudianos et al, 2006;Yamamoto et al, 2005Yamamoto et al, , 2006Yamamoto et al, , 2007.…”