Glutathione S-transferases related to P. aeruginosa lung infection in cystic fibrosis children: Preliminary study

Feuillet‐Fieux, Marie‐Noëlle; Nguyen–Khoa, Thao; Loriot, Marie‐Anne; Villartay, Philippe de; Sermet, Isabelle; Verrier, P. E.; Bonnefont, Jean‐Paul; Beaune, Philippe; Lenoir, G.; Lacour, Bernard

doi:10.1016/j.clinbiochem.2008.09.116

Cited by 16 publications

(13 citation statements)

References 32 publications

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“…This result is in contrast with that of Feuillet-Fieux et al [40] who found no differences in the level of GST activity in blood between children with CF and healthy group. GST biosynthesis is upregulated by activation of Nrf2 factor by oxidative/electrophile stress [41].…”

Section: Discussioncontrasting

confidence: 99%

Oxidative Modification of Proteins in Pediatric Cystic Fibrosis with Bacterial Infections

Sadowska-Bartosz

Galiniak

Bartosz

et al. 2014

Oxidative Medicine and Cellular Longevity

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Pseudomonas aeruginosa and Staphylococcus aureus cause chronic lung infection in cystic fibrosis (CF) patients, inducing chronic oxidative stress. Several markers of plasma protein oxidative damage and glycoxidation and activities of erythrocyte antioxidant enzymes have been compared in stable CF patients chronically infected with Pseudomonas aeruginosa (n = 12) and Staphylococcus aureus (n = 10) in relation to healthy subjects (n = 11). Concentration of nitric oxide was also measured in the exhaled air from the lower respiratory tract of patients with CF. Elevated glycophore (4.22 ± 0.91 and 4.19 ± 1.04 versus control 3.18 ± 0.53 fluorescence units (FU)/mg protein; P < 0.05) and carbonyl group levels (1.9 ± 0.64, 1.87 ± 0.45 versus control 0.94 ± 0.19 nmol/mg protein; P < 0.05) as well as increased glutathione S-transferase activity (2.51 ± 0.88 and 2.57 ± 0.79 U/g Hb versus 0.77 ± 0.16 U/g Hb; P < 0.05) were noted in Pseudomonas aeruginosa and Staphylococcus aureus infected CF. Kynurenine level (4.91 ± 1.22 versus 3.89 ± 0.54 FU/mg protein; P < 0.05) was elevated only in Staphylococcus aureus infected CF. These results confirm oxidative stress in CF and demonstrate the usefulness of the glycophore level and protein carbonyl groups as markers of oxidative modifications of plasma proteins in this disease.

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Section: Discussioncontrasting

confidence: 99%

Oxidative Modification of Proteins in Pediatric Cystic Fibrosis with Bacterial Infections

Sadowska-Bartosz

Galiniak

Bartosz

et al. 2014

Oxidative Medicine and Cellular Longevity

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“…Feuillet--Fieux et al (2009), comparing antioxidant markers concentrations -including vitamin A in healthy subjects (n = 9) and CF patients infected (n = 12) and uninfected (n = 24) Pseudomonas aeruginosa -found no significant differences in vitamin A levels between groups. Maqbool et al (2008) showed that serum retinol levels were negatively correlated with standardized body weight and height, and not associated with age, gender or vitamin A intake (from food, supplement or both).…”

Section: Discussionmentioning

confidence: 99%

Vitamin A status and its determinants in patients with cystic fibrosis

Sapiejka¹,

Krzyżanowska

Walkowiak

et al. 2017

Acta Sci Pol Technol Aliment

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Background. Routine administration of vitamin A, recommended in CF patients, can help to prevent its deficiency. However, high vitamin A supplementation may lead to its excessive level and possible toxicity. Therefore, the aim of the present study was to assess the status of vitamin A and the determinants of its body resources in CF patients. Material and methods. In 196 CF patients aged from 4 months to 47 years, the following parameters were analysed: nutritional status (standardized body weight and height, serum albumin concentration) and clinical expression of disease (lung function − spirometry; biochemical markers of liver function − ALT, AST, GGT; respiratory tract colonization by Pseudomonas aeruginosa; diabetes; cirrhosis, non-cirrhotic liver disease; exocrine pancreatic function − fecal elastase-1 concentration; blood clotting -INR and vitamin A supplementation). Results. Median vitamin A concentration in the study group was 383.0 ng/ml (1

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“…It is a crucial part of the intracellular defense system, which protects the epithelium against the injuries and inflammation [17] common to CF that are caused by oxidation [18]. As polymorphisms can alter the GSH metabolic pathway, genetic variations of this pathway have previously been studied in association with CF [19-21]. …”

Section: Introductionmentioning

confidence: 99%

“…Of these genes, GSTP1 is associated with hepatic disease [19] and infection [20], GSTM1 with greater CF clinical severity [21], GSTT1 with no CF clinical variables, while GCLC has not been previously studied in relation to CF. However, as the action of the GSH protein is closely related to that of CFTR [29], it is conceivable that GCLC and GST polymorphisms influence CF severity [19-21,26,30].…”

Section: Introductionmentioning

confidence: 99%

“…However, as the action of the GSH protein is closely related to that of CFTR [29], it is conceivable that GCLC and GST polymorphisms influence CF severity [19-21,26,30]. This study therefore aimed to determine whether genetic polymorphisms in the GSH metabolic pathway are associated with CF severity under different phenotypes of the disease.…”

Section: Introductionmentioning

confidence: 99%

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Polymorphisms in the glutathione pathway modulate cystic fibrosis severity: a cross-sectional study

et al. 2014

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BackgroundCystic fibrosis (CF) clinically manifests with various levels of severity, which are thought to be modulated by mutations in the cystic fibrosis transmembrane conductance regulator gene (CFTR), modifier genes, and the environment. This study verified whether polymorphisms in modifier genes associated with glutathione (GSH) metabolism influence CF severity.MethodsA cross-sectional study of 180 CF patients was carried out from 2011 to 2012. We analyzed CFTR mutations, polymorphisms (GSTM1 and GSTT1 deletions, GSTP1 + 313A > G, GCLC-129C > T, and GCLC-3506A > G) in modifier genes and CF clinical severity as assessed by 28 clinical and laboratory variables.ResultsSignificant associations were found between modifier gene polymorphisms and particular phenotypes or genotype changes. These included GCLC-129C > T with a higher frequency of the Pseudomonas aeruginosa mucoid to CC genotype (p = 0.044), and GCLC-3506A > G with a higher frequency of the no-mucoid P. aeruginosa (NMPA) to AA genotype (p = 0.012). The GSTT1 deletion was associated with a higher frequency of the NMPA to homozygous deletion (p = 0.008), GSTP1 + 313A > G with a minor risk of osteoporosis (p = 0.036), and patient age ≤ 154 months (p = 0.044) with the AA genotype. The Bhalla score was associated with GCLC-3506A > G (p = 0.044) and GSTM1/GSTT1 deletion polymorphisms (p = 0.02), while transcutaneous hemoglobin oxygen saturation levels were associated with GSTT1 deletions (p = 0.048).ConclusionCF severity is associated with polymorphisms in GSH pathways and CFTR mutations.

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Glutathione S-transferases related to P. aeruginosa lung infection in cystic fibrosis children: Preliminary study

Cited by 16 publications

References 32 publications

Oxidative Modification of Proteins in Pediatric Cystic Fibrosis with Bacterial Infections

Oxidative Modification of Proteins in Pediatric Cystic Fibrosis with Bacterial Infections

Vitamin A status and its determinants in patients with cystic fibrosis

Polymorphisms in the glutathione pathway modulate cystic fibrosis severity: a cross-sectional study

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