Glutathionyl hemoglobin in uremic patients undergoing hemodialysis and continuous ambulatory peritoneal dialysis

Abstract

Glutathionyl Hb levels were increased in HD and CAPD patients, probably because of enhanced oxidative stress. The measurement of glutathionyl Hb may be useful to assess oxidative stress in uremic patients.

Protein glutathionylation and oxidative stress

“…3). Glutathionyl Hb levels in hemodialysis patients (8.0 ± 3.6%; n = 30; p < 0.0001) and continuous ambulatory peritoneal dialysis patients (5.9 ± 2.7%; n = 10; p < 0.05) were significantly elevated as compared with normal subjects (3.0 ± 1.6%; n = 20) [25]. However, there were no significant differences in glutathionyl Hb between hemodialysis patients and continuous ambulatory peritoneal dialysis patients.…”

S‐Glutathionylation: from redox regulation of protein functions to human diseases

“…Since the blood concentration of PSSGs may reflect alterations in the oxidative stress status even in hardly accessible tissues and compartments of the body, haemoglobin has been analysed in some human diseases as a marker of whole-body oxidative stress. Glutathionyl-haemoglobin is increased in patients suffering from type I and type II diabetes, Friedreich's ataxia, hyperlipidemia, and uremia associated with haemodialysis or peritoneal dialysis [20,58,[61][62][63]. Differently, a decrease in the normal (basal) concentration of glutathionylhaemoglobin has been measured in children with Down's syndrome [64].…”

Hemoglobin glutathionylation can occur through cysteine sulfenic acid intermediate: Electrospray ionization LTQ-Orbitrap hybrid mass spectrometry studies

“…Among others, Hb-SSG is now recognized as a promising bio-marker of systemic oxidative stress [3]. Significant increase of Hb-SSG content, in respect to its low value in healthy individuals, has been found in several oxidative stress-based diseases such as diabetes, hyperlipidemia, Friedreich ataxia and chronic renal failure [4][5][6][7][8].…”