Gly-Pro and Adaptive Reactions in Multicomponent Stress

Tomova, Tatiana; Замощина, Т. А.; Svetlik, M. V.; Седокова, Марина Львовна; Gostyukhina, Alеna; Fatyushina, A. M.

doi:10.1007/s11055-020-01009-7

Cited by 2 publications

(1 citation statement)

References 21 publications

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“…Тварини у «відкритому полі» знаходяться під впливом стресу, на який вони реагують по-різному, що відображається на їхній руховій активності, стані автономної нервової діяльності [8]. Особливості поведінки у даному тесті дають змогу оцінити рухову, дослідницьку, емоційну реакції, виявити особин із високою і низькою руховою активністю, стійких та нестійких до емоційного стресу тварин, зробити прогноз індивідуальної стійкості до стресу [9].…”

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Effect of stress on the development of lipid peroxide oxidation in rats with different motor activity

Denefil,

Medynskyi

2022

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Summary. Today in Ukraine and in the world the study of the stress problem has become especially acute. Firstly, it is connected with sedentary lifestyle during the COVID-19 pandemic, and secondly, in connection with the war in Ukraine, in which the whole world is involved. Both causes contribute to the development of stress, which will lead to further diseases of various organs and systems. The aim of the study – to evaluate the development of oxidative stress in the blood of male rats with different motor activity under stress. Materials and Methods. The experiments performed on Wistar male-rats of 150– 170 grams, aged 3.5 months. Selection of animals for motor activity carried out using the method of "white open field". The number of horizontally intersected squares and vertical posts taken into account. At high level indicators were referred to were the group of highly active animals, at low – low-active. Chronic stress in rats was from 1.5 to 3 months of age, the animals kept in cages with limited living space twice. Slaughter of rats was performed under thiopental-sodium anesthesia, blood was taken, where the diene conjugates (DC), TBA-active products (TBA-ap), oxidatively modified proteins (OMP), superoxide dismutase (SOD) and catalase activity where determined. All animals underwent histological examination of the heart at the level of both ventricles in Heidenhain-stained preparations. Apoptosis was predominant in animals with high motor activity in both control rats and stressed animals. The number of cells that underwent apoptosis was significantly higher in highly active animals. Results. Products of lipid peroxidation and OMP, SOD and catalase activity were dominated in the group of control animals in highly active males, compared with low-activity. In stressed rats, the DC, TBA-ap, OMP increased, and were more in highly active rats. Antioxidant activity increased in stressed animals. SOD and catalase activity were higher in low-activity rats The obtained data indicate the development of oxidative stress. Conclusion. The development of stress depends on the motor activitys. Stress causes an increase of proteins and lipid peroxidation in rats, which is more in high motor activity. The activity of antioxidants, more in low-activity individuals increased compensatory in the blood

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Effect of stress on the development of lipid peroxide oxidation in rats with different motor activity

Denefil,

Medynskyi

2022

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Untargeted Metabolomics of Korean Fermented Brown Rice Using UHPLC Q-TOF MS/MS Reveal an Abundance of Potential Dietary Antioxidative and Stress-Reducing Compounds

et al. 2021

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Free radical-induced oxidative stress is the root cause of many diseases, such as diabetes, stress and cardiovascular diseases. The objective of this research was to screen GABA levels, antioxidant activities and bioactive compounds in brown rice. In this study, we first fermented brown rice with different lactic acid bacteria (LABs), and the best LAB was selected based on the levels of GABA in the fermentate. Lactobacillus reuterii generated the highest levels of GABA after fermentation. To ascertain whether germination can improve the GABA levels of brown rice, we compared the levels of GABA in raw brown rice (Raw), germinated brown rice (Germ), fermented brown rice (Ferm) and fermented-germinated brown rice (G+F) to identify the best approach. Then, antioxidant activities were investigated for Raw BR, Germ BR, Ferm BR and G+F BR. Antioxidant activity was calculated using a 2,2-diphenyl-1-picryl hydrazile radical assay, 2,2-azino-bis-(3-ethylene benzothiozoline-6-sulfonic acid) radical assay and ferric-reducing antioxidant power. In Ferm BR, DPPH (114.40 ± 0.66), ABTS (130.52 ± 0.97) and FRAP (111.16 ± 1.83) mg Trolox equivalent 100g, dry weight (DW), were observed as the highest among all samples. Total phenolic content (97.13 ± 0.59) and total flavonoids contents (79.62 ± 1.33) mg GAE/100 g and catechin equivalent/100 g, DW, were also found to be highest in fermented BR. Furthermore, an untargeted metabolomics approach using ultra-high-performance liquid tandem chromatography quadrupole time of flight mass spectrometry revealed the abundance of bioactive compounds in fermented BR, such as GABA, tryptophan, coumaric acid, L-ascorbic acid, linoleic acid, β-carotenol, eugenol, 6-gingerol, etc., as well as bioactive peptides which could contribute to the health-promoting properties of L. reuterii fermented brown rice.

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Gly-Pro and Adaptive Reactions in Multicomponent Stress

Cited by 2 publications

References 21 publications

Effect of stress on the development of lipid peroxide oxidation in rats with different motor activity

Effect of stress on the development of lipid peroxide oxidation in rats with different motor activity

Untargeted Metabolomics of Korean Fermented Brown Rice Using UHPLC Q-TOF MS/MS Reveal an Abundance of Potential Dietary Antioxidative and Stress-Reducing Compounds

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