Glyaderm® dermal substitute: Clinical application and long-term results in 55 patients

Pirayesh, Ali; Hoeksema, Henk; Richters, C. D.; Verbelen, Jozef; Monstrey, Stan

doi:10.1016/j.burns.2014.05.013

Cited by 47 publications

(47 citation statements)

References 45 publications

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“…Glycerol preserved acellular dermis (Glyaderm 1 ) consists of collagen and elastin fibers and is the first non-profit dermal substitute derived from glycerol-preserved, human allogeneic skin [4,5,48].…”

Section: Discussionmentioning

confidence: 99%

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Exposed tibial bone after burns: Flap reconstruction versus dermal substitute

Verbelen

Hoeksema

Pirayesh

et al. 2016

Burns

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Section: Discussionmentioning

confidence: 99%

“…Sodium hydroxide (NaOH) is used to decellularize the GPA, resulting in a scaffold of human collagen and elastin. At present Glyaderm 1 is mainly indicated for bi-layered skin reconstruction of full thickness wounds [4,5].…”

mentioning

confidence: 99%

Exposed tibial bone after burns: Flap reconstruction versus dermal substitute

Verbelen

Hoeksema

Pirayesh

et al. 2016

Burns

Self Cite

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“…[6][7][8] However, because it only functionally serves to replace the dermal layer, epidermal coverage in the form of a split-thickness skin graft is still needed. Regardless, numerous studies have demonstrated that the addition of acellular dermal matrix use in this setting results in a faster healing time, 9,10 better aesthetic outcome with improved skin elasticity, and reduced scar contracture [11][12][13][14][15][16][17][18][19] ; can serve as a temporizing measure to avert the need for emergency surgery 19 ; and allows improved mobility and function of joints that require skin coverage. 10,[12][13][14]18 Although there are clear benefits to its use, acellular dermal matrix will never serve as a complete replacement for autologous tissue transfer in this setting, and more prospective data are needed (Table 2).…”

Section: Basic Sciencementioning

confidence: 99%

Regenerative Biomaterials

Banyard

Bourgeois

Widgerow

et al. 2015

Plastic and Reconstructive Surgery

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The authors present a review of biomaterials, substances traditionally derived from human or animal tissue or, more recently, biodegradable synthetics modeled after naturally occurring resources. These constructs differ from purely synthetic materials in that they are degraded or incorporated into a host's tissue. These biomaterials include a diverse array of medical products, such as acellular dermal matrix, bone substitutes, and injectables. In this review, the authors examine various clinical applications, including burn reconstruction and wound healing, breast surgery, complex abdominal wall reconstruction, craniofacial repair, and cosmetic surgery. Biomaterials such as acellular dermal matrix have proven beneficial in difficult-to-treat applications; however, more prospective data are needed to determine their true efficacy and cost-effectiveness.

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“…A number of constructs are already being used to treat large burns and ulcers, for example decellularized human dermis (Glyaderm® [13]), artificially made acellular dermal template (Integra® [14,15]) dermal substitutes containing fibroblasts (Dermagraft® [16]) and full-thickness skin substitutes (allogeneic Apligraf® [17]; autologous Tiscover® [5,18]). Although the results are very promising there is room for improvement with regards to vascularization.…”

Section: Introductionmentioning

confidence: 99%

“…In all cases, graft take is reliant on fast ingrowth of new vessels (angiogenesis) once the construct is placed on the wound bed. In the case of dermal templates, vascularization of the construct is required before a split-thickness autograft can be applied on top of the dermal template [13–15]. Improving the rate of vascularization would enhance graft take and result in faster wound closure.…”

Section: Introductionmentioning

confidence: 99%

Extensive Characterization and Comparison of Endothelial Cells Derived from Dermis and Adipose Tissue: Potential Use in Tissue Engineering

et al. 2016

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Tissue-engineered constructs need to become quickly vascularized in order to ensure graft take. One way of achieving this is to incorporate endothelial cells (EC) into the construct. The adipose tissue stromal vascular fraction (adipose-SVF) might provide an alternative source for endothelial cells as adipose tissue can easily be obtained by liposuction. Since adipose-EC are now gaining more interest in tissue engineering, we aimed to extensively characterize endothelial cells from adipose tissue (adipose-EC) and compare them with endothelial cells from dermis (dermal-EC). The amount of endothelial cells before purification varied between 4–16% of the total stromal population. After MACS selection for CD31 positive cells, a >99% pure population of endothelial cells was obtained within two weeks of culture. Adipose- and dermal-EC expressed the typical endothelial markers PECAM-1, ICAM-1, Endoglin, VE-cadherin and VEGFR2 to a similar extent, with 80–99% of the cell population staining positive. With the exception of CXCR4, which was expressed on 29% of endothelial cells, all other chemokine receptors (CXCR1, 2, 3, and CCR2) were expressed on less than 5% of the endothelial cell populations. Adipose-EC proliferated similar to dermal-EC, but responded less to the mitogens bFGF and VEGF. A similar migration rate was found for both adipose-EC and dermal-EC in response to bFGF. Sprouting of adipose-EC and dermal-EC was induced by bFGF and VEGF in a 3D fibrin matrix. After stimulation of adipose-EC and dermal-EC with TNF-α an increased secretion was seen for PDGF-BB, but not uPA, PAI-1 or Angiopoietin-2. Furthermore, secretion of cytokines and chemokines (IL-6, CCL2, CCL5, CCL20, CXCL1, CXCL8 and CXCL10) was also upregulated by both adipose- and dermal-EC. The similar characteristics of adipose-EC compared to their dermal-derived counterpart make them particularly interesting for skin tissue engineering. In conclusion, we show here that adipose tissue provides for an excellent source of endothelial cells for tissue engineering purposes, since they are readily available, and easily isolated and amplified.

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Glyaderm® dermal substitute: Clinical application and long-term results in 55 patients

Cited by 47 publications

References 45 publications

Exposed tibial bone after burns: Flap reconstruction versus dermal substitute

Exposed tibial bone after burns: Flap reconstruction versus dermal substitute

Regenerative Biomaterials

Extensive Characterization and Comparison of Endothelial Cells Derived from Dermis and Adipose Tissue: Potential Use in Tissue Engineering

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