Glyburide inhibits the bone resorption induced by traumatic occlusion in rats

Abstract

Objective To examine whether glyburide inhibits bone destruction caused by traumatic occlusion in a rat occlusal trauma model. Background Excessive mechanical stress, such as traumatic occlusion, induces expression of IL‐1β and may be involved in bone resorption. NLRP3 inflammasomes have been linked to IL‐1β expression, but it is currently unclear whether glyburide, the inhibiter of NLRP3 inflammasome, suppresses occlusal trauma in rats. Methods Male SD rats aged 7 weeks were used. In the trauma group, the occlusal surface of the maxillary first right molar was raised by attaching a metal wire to apply occlusal trauma to the mandibular first right molar. In the trauma + glyburide group, the NLRP3 inhibitor glyburide was administered orally every 24 hours from 1 day before induction of occlusal trauma. Rats were euthanized after 5 or 10 days, and the maxillary first molars were harvested with the adjacent tissues for histopathological investigation. Immunohistochemical expression of IL‐1β, NLRP3, and RANKL was also assessed. Results On day 5, bone resorption was significantly greater in the trauma group compared with the control group or the trauma + glyburide group, and there were significantly higher numbers of osteoclasts and cells positive for IL‐1β, NLRP3, and RANKL in the trauma group. Conclusion In this study, glyburide inhibits bone resorption by traumatic occlusion in rats. It suggests that the NLRP3/IL‐1β pathway might be associated with bone resorption induced by traumatic occlusion.

IL-1 Superfamily Members and Periodontal Diseases

“…Excessive mechanical stress, including orthodontic movement and occlusal trauma, causes hyaline degeneration and can activate NLRP3 inflammasomes, increasing IL-1β expression in the periodontal apparatus. Glyburide, an NLRP3 inflammasome inhibitor, inhibited bone resorption by traumatic occlusion in rats by decreasing IL-1β tissue levels (Arita et al 2020).…”

Study on the role of pyroptosis in bone resorption induced by occlusal trauma with or without periodontitis

“…The qRT‐PCR and IHC results confirmed a sustained traumatic occlusal force throughout the experiment. Compared to the previously reported occlusal trauma experimental model, 22–27 this occlusal trauma model avoids several interference factors, such as resin abrasion, bad bonding and fixation of orthodontic wire, as well as the temporomandibular joint disorder in the experimental animals. Furthermore, the occlusal trauma model induced by metal crown bonding mimicked the clinical occlusal trauma disease.…”

“…NOD‐like receptor pyrin domain containing protein 3, as the upstream effector of caspase‐1, is considered to be another key molecule in pyroptosis, since it plays a key role in classical pyroptosis as a sensor of inflammation 43 . Recent studies have shown that glyburide inhibits bone resorption induced by occlusal trauma 22 . Here, we proposed a novel hypothesis stating that the clinical occlusal trauma with periodontitis leads to alveolar bone resorption through the NLRP3/caspase‐1/IL‐1β pathway.…”

Annual review of selected scientific literature: A report of the Committee on Scientific Investigation of the American Academy of Restorative Dentistry

“…NLRP3 inflammasomes have been linked to IL1b expression. Arita et al 254 examined whether glyburide could suppress occlusal trauma in rats (age 7 weeks). In the trauma group, wire was attached to maxillary right first molar occlusal surfaces resulting in occlusal trauma with mandibular right first molars.…”