Glycan Analysis by Ion Mobility–Mass Spectrometry

Hofmann, Johanna; Pagel, Kevin

doi:10.1002/anie.201701309

Cited by 158 publications

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“…As compared to other biomolecules (e.g., nucleic acids, peptides), glycan synthesis is performed in a non-template driven fashion, where their composition can be varied according to immunological responses, cellular signaling, and disease state 1 . This variation in glycan composition, termed isomeric heterogeneity, leads to numerous potential structural permutations (e.g., regioisomers, stereoisomers, and diastereomers), further complicating analyses 2 . This isomeric heterogeneity is further magnified by added structural permutations with key examples including glycosidic linkage position (e.g., 1–4 versus 1–6 linkage), α/β anomericity, and monosaccharide subunit composition (e.g., galactose versus glucose), as well as variation conformers (e.g., five-membered furanose versus six-membered pyranose rings), as shown in Figure 1.…”

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confidence: 99%

“…Chemical derivatization is often a prerequisite for reversed-phase separations so as to increase the hydrophobicity of the inherently polar glycans 1 , 2 . While reversed-phase separations remain the preferred choice in glycan separations, HILIC or normal stationary phases have seen some recent use 2 . Even with the growing suite of MS n methods, the deconvolution and identification of co-eluting species remains a challenge since isomers exhibit very similar fragmentation pathways 4 , 5 .…”

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Unraveling the isomeric heterogeneity of glycans: ion mobility separations in structures for lossless ion manipulations

et al. 2018

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To address the challenges associated with glycan analyses, we have implemented a structures for lossless ion manipulations (SLIM) serpentine ultra-long path with extended routing (SUPER) ion mobility-mass spectrometry (i.e. SLIM SUPER IM-MS) platform to achieve much higher resolution of isomeric glycoforms. We have demonstrated the potential of this platform as a future component of the glycomics toolbox.

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Unraveling the isomeric heterogeneity of glycans: ion mobility separations in structures for lossless ion manipulations

et al. 2018

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“…Host-secreted glycans are consisted of oligosaccharides synthesised in a non-template-driven fashion. Hence, they are often branched and show complex regio-and stereo-chemistry resulting in an enormous number of sugar structures in human body (Hofmann and Pagel 2017).…”

Section: Contemplating the Complexity Of The Gut Microbiota And Host-mentioning

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Cross-feeding interactions of gut symbionts driven by human milk oligosaccharidesand mucins

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Chapter 4Microbial metabolic networks at the mucus layer lead to dietindependent butyrate and vitamin B12 production by intestinal symbionts 71 Chapter 5Deciphering trophic interaction between Akkermansia muciniphila and the butyrogenic gut commensal Anaerostipes caccae using a metatranscriptomic approach 97 Chapter 6General discussion 123 Chapter 7 Thesis summary & Nederlandse samenvatting 143 Appendices References 153Co-author affiliations 179Acknowledgements 183About the author 184List of publications 185 VLAG training activities 186Chapter 1 General introduction and thesis outlineChapter 1 2 General introductionHumans can be considered holobionts, consisting of cells from the host and an even larger number of microorganisms (Postler and Ghosh 2017). The majority of microbes are residing in the gut (Sender et al 2016). The human gut microbiota is a complex ecosystem consisting of bacteria, archaea, microeukaryotes and viruses (Clemente et al 2012). Over 2000 species-level phylotypes (prokaryotes including bacteria and archaea) have been detected for the gut microbiota, with each individual estimated to host at least 160 species (Qin et al 2010, Zoetendal et al 2008. The gut microbiota may impact the well-being of human and susceptibility for diseases by interacting with our metabolic, immune and neurological system (Honda and Littman 2016, Koh et al 2016, Rogers et al 2016. Furthermore, the gut microbiota contributes to a variety of metabolic functions and can be seen as an essential part of our digestive system (El Kaoutari et al 2013). Bacterial symbionts allow the human hosts to utilise inaccessible nutrients by converting complex substrates to short chain fatty acid (SCFA) and vitamins (Fisher et al 2017). Bacterial-derived SCFAs are estimated to contribute about 10% of our daily caloric requirement (Bergman 1990). While all SCFAs are vital for maintaining gut homeostasis, butyrate is of particular interest because it has been attributed to a range of health-promoting functions. Butyrate is the primary energy source for colonic epithelial cells and is associated with the enhancement of colonic barrier function, increase of satiety, pain relief, anti-inflammatory responses, and protection against colorectal cancer (Banasiewicz et al 2013, Bolognini et al 2016, Donohoe et al 2011, Furusawa et al 2013, Goncalves and Martel 2013.Considering the physiological benefits of butyrate to human health, this thesis investigates the microbial interaction of gut symbionts that support butyrate production driven primarily by the host produced glycans in human milk and mucus. Glycans are compounds consisting of an array of glycosidically linked monosaccharides (monosaccharides and disaccharides are collectively termed sugars) such as human milk oligosaccharides (HMOS) and mucins (Varki and Kornfeld 2017). HMOS present in mother milk are the major compositional and functional driver of the infant gut microbiota (Backhed et al 2015). Mucin glycans covering the intestinal lining create a stable niche for bacterial coloni...

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“…Chiral analysis has been attempted using mass spectrometry by generating chiral environment, performing tandem MS experiment or relying on kinetic method [9]. Recent years, ion mobility mass spectrometry (IM-MS) has been successfully utilized in the isomer analysis using specific collision cross section (CCS) ions [10]. Since the complicated composition of lipids and the existence of lipid isomers, separation and identification of isomers are still the most challenging work in complex samples.…”

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Supercritical Fluid Chromatography and Its Application in Lipid Isomer Separation

2017

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Supercritical fluid chromatography (SFC), which uses supercritical fluid with higher diffusivity and lower viscosity than liquid as its mobile phase, can provide fast, green, high-throughput and good performance methods for the analysis of complicated samples. It is an advantageous alternative for the separation of polar and nonpolar compounds, and even chiral isomers. As an important constituent of living organisms, lipids and the related lipidomics researches have attracted dramatic attention during the past years. The separation and identification of lipids and lipid isomers are of great significance and remain challenging. In this mini-review, the principle of SFC and its applications in lipid isomer separation are summarized.

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Glycan Analysis by Ion Mobility–Mass Spectrometry

Cited by 158 publications

References 89 publications

Unraveling the isomeric heterogeneity of glycans: ion mobility separations in structures for lossless ion manipulations

Unraveling the isomeric heterogeneity of glycans: ion mobility separations in structures for lossless ion manipulations

Cross-feeding interactions of gut symbionts driven by human milk oligosaccharidesand mucins

Supercritical Fluid Chromatography and Its Application in Lipid Isomer Separation

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