Glycan analysis of recombinant Aspergillus niger endo-polygalacturonase A

Woosley, Bryan; Kim, Young Hwan; Kolli, V. S. Kumar; Wells, Lance; King, Daniel; Poe, Ryan; Orlando, Ron; Bergmann, Carl

doi:10.1016/j.carres.2006.06.006

Cited by 13 publications

(5 citation statements)

References 23 publications

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“…We have previously discovered that several of the EPGs we have studied (PG I, PGA, PGC from Aspergillus niger, along with PG 2 and PG 3 from Botrytis cinerea) contain O-linked mannose. [31][32][33] These modifications are located near the N-terminus in close proximity to the sites where we hypothesize PGIP binding occurs. This has led us to speculate that O-mannosylation may play an important role in EPG/PGIP interaction, as the O-Man-initiated modification has been previously observed to have a dramatic impact on the R-dystroglycan/laminin interaction in animals.…”

Section: Discussionmentioning

confidence: 85%

“…These single site changes are unlikely to account for the wide spectrum of binding and inhibitory activities observed, and little emphasis has been placed on the potential for glycosylation to effect the ability of PGIPs to bind, and/or to inhibit EPGs. We have previously discovered that several of the EPGs we have studied (PG I, PGA, PGC from Aspergillus niger , along with PG 2 and PG 3 from Botrytis cinerea ) contain O-linked mannose. − These modifications are located near the N-terminus in close proximity to the sites where we hypothesize PGIP binding occurs. This has led us to speculate that O-mannosylation may play an important role in EPG/PGIP interaction, as the O-Man-initiated modification has been previously observed to have a dramatic impact on the α-dystroglycan/laminin interaction in animals …”

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 98%

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Mapping Glycans onto Specific N-Linked Glycosylation Sites of Pyrus communis PGIP Redefines the Interface for EPG−PGIP Interactions

et al. 2008

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Polygalacturonase inhibiting proteins (PGIPs) are members of the leucine rich repeat family of proteins, involved in plant defense against fungal pathogens. PGIPs exhibit a remarkable degree of specificity in terms of their ability to bind and inhibit their target molecules, the endopolygalacturonases (EPGs). This specificity has been attributed for certain EPG/PGIP combinations to differences in primary sequence, but this explanation is unable to account for the full range of binding and inhibitory activities observed. In this paper we have fully characterized the glycosylation on the PGIP derived from Pyrus communis and demonstrated, using a combination of PNGaseF and PNGaseA in 18O-water, that the Pyrus communis PGIP utilizes all seven potential sites of N-linked glycosylation. Further, we demonstrate that certain sites appear to be modified only by glycans bearing α3-linked core fucosylation, while others are occupied by a mixture of fucosylated and non-fucosylated glycans. Modeling of the carbohydrates onto a homologous structure of PGIP indicates potential roles for glycosylation in mediating the interactions of PGIPs with EPGs.

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Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 98%

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Mapping Glycans onto Specific N-Linked Glycosylation Sites of Pyrus communis PGIP Redefines the Interface for EPG−PGIP Interactions

et al. 2008

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“…Desalting is often accomplished by filtration (19, 20), dialysis (21, 22), cation exchange resin (23, 24), or carbon-based column adsorption (11, 13, 16, 18). …”

Section: Glycomics Methodsmentioning

confidence: 99%

Glycomics and disease markers

Kronewitter

Leoz

et al. 2009

Current Opinion in Chemical Biology

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“…Figure 12 shows a possible general structure deduced from the ESI-MS/MS spectra in which AG indicates the aglycon unit. In some cases, based on the literature (Bao-Shiang et al, 2005;Woosley et al, 2006;Zhang and Chelius, 2004), it has been possible to propose the identity of the the AG unit (i.e. Intens.…”

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Parámetros de calidad del agua relacionados con la presencia de materia orgánica y microorganismos

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A mi familia AGRADECIMIENTOSUna vez llegado al final de la tesis y echando la mirada atrás me doy cuenta de toda la gente a la que le tengo algo que agradecer, y que de una manera u otra ha contribuido a la realización de este trabajo. En primer lugar, quería agradecer a Hermenegildo García Gómez y a MercedesÁlvaro Rodríguez la posibilidad de haber trabajado en su grupo de investigación.A la Universidad Politécnica de Valencia por haberme concedido una beca/contrato del Programa Cantera de la UPV.Al Professor Simon Parsons y a la Dr. Emma Goslan del Centre for Water Science (Cranfield University, UK) por haberme permitido trabajar y disfrutar unos meses con todos ellos.A la gente de Aguas de Valencia por su apoyo cuando lo he necesitado. En especial nombrar al laboratorio de la planta potabilizadora de La Presa (Lorenzo, Paco, Patricia, Filo, Jose, Sonia…), así como a los laboratorios GAMASER.Agradecer también al Departamento de Ingeniería Química de la UPV, y en concreto a Isabel Alcaina la oportunidad de haber colaborado con ellos durante cierto tiempo en la planta de membranas.También quería agradecer su ayuda y compañía a la gente del Departamento de Química. Llevo tanto tiempo aquí que de cada uno podría escribir muy buenos recuerdos.A mis compañeros del Laboratorio 2-5, y especialmente a Javi, Miriam, Belén, Esther, Bogdan etc. en mis inicios en este mundillo y a las nuevas generaciones con Roberto, Maykel, Mireia, Murthy, David1 y David2 con los que hemos pasado muchos, y muy buenos momentos. También agradecer a todos los de mi grupo del ITQ su ayuda en cualquier cosa que me hace falta (Raquel, Canijo, Fran, Xesc, Ana, Nacho, Laura, Esther, Abde, Pedro, Abad etc.).A todos los componentes de las primeras cervecitas, las segundas y las que nos seguimos tomando… los del 2-5, los del 2-6, los del 2-2 y todos los que hemos ido a disfrutar de la química de los alimentos, con esas conversaciones que a veces se alargan hasta las tantas de un día cualquiera. Especialmente Nacho, Sergio, Alberto, Pavel, Cal-lo, Nour, Liliana, Paula, Inma, Andrea, Felix, Tatiana… y ,aunque no vienen tanto como me gustaría, Raúl, Luis, Chelo etc.Recordar también a toda la gente que he conocido en los congresos especialmente Virginia, Antonio, Juan etc. con los que he compartido muy buenos momentos.A todos mis amigos de Valencia y de Moixent que con esas paellas, cenas, cervecitas y demás le alegran la vida a uno.Agradecer a mis familiares los consejos recibidos y en concreto a mis tíos Juan José y Manolo Camarasa.Muy especialmente, agradecer todo el apoyo incondicional y entendimiento recibido a lo largo de toda mi vida por parte de mis padres, mi hermana, mi abuela, mi cuñado y de mi sobrinita Sandra, sin los que nada de esto hubiese sido posible. Muchas gracias a todos! PARTE IV. MECANISMOS DE FORMACIÓN DE THMsIV.1 Formación de THMs a partir de compuestos fenólicos IV.2 Formación de THMs a partir de compuestos ȕ-dicarbonílicos IV.3 Cloración de compuestos nitrogenados IV.4 Variables que afectan a la formación de THMs IV.5 Precurso...

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Glycan analysis of recombinant Aspergillus niger endo-polygalacturonase A

Cited by 13 publications

References 23 publications

Mapping Glycans onto Specific N-Linked Glycosylation Sites of Pyrus communis PGIP Redefines the Interface for EPG−PGIP Interactions

Mapping Glycans onto Specific N-Linked Glycosylation Sites of Pyrus communis PGIP Redefines the Interface for EPG−PGIP Interactions

Glycomics and disease markers

Parámetros de calidad del agua relacionados con la presencia de materia orgánica y microorganismos

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