Glycan and lectin biosensors

Belický, Štefan; Katrlı́k, Jaroslav; Tkáč, Ján

doi:10.1042/ebc20150005

Cited by 58 publications

(32 citation statements)

References 46 publications

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“…Progress in membrane and solid-phased technologies using LS-GC interactions includes the potential of their application in microassays, biochips (membrane bound glycoarrays or lectin arrays), and biosensors [35,36].…”

Section: Membrane Technological Prospects Of Lssmmentioning

confidence: 99%

Metabolite Multiprobiotic Formulas for Microbial Health

Lakhtin¹,

Lakhtin²,

Алешкин³

et al. 2019

Oral Health by Using Probiotic Products

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On example of Lactobacillus and Bifidobacterium strains, approaches in creation of human multistrain probiotic metabolite mixtures for different goals were proposed. Human probiotic lectin systems (LS) (mucosal, others) reveal functions needed for organism. Advanced features of such systems include capability to recognize synthetic polymeric polyvalent glycoconjugates (GC)-imitators of natural ones (modified polysaccharides, glycoantigens of medical significance). Probiotic lectin systems function as imitators of multipro-/sym-/synbiotics in their resulting actions. They serve as carriers of the biotope glycoconjugate décor including glycoprebiotics, glycometabiotics, glycodrugs, and agents supporting décor organization and resistance. Probiotic lectin systems represent new perspective system agents to improve the health of mucosal microbiocenoses (MB) organized as communicative bodies to be corrected according to the principle "there is bodythere are diseases." They act as metabolomebiotics according to the principle "the network in the network." They deepen biotope resistance allowing quicker return to balance. They support prophylactic and therapeutic procedures directed to prolong aging and improve quality of life. Multistrain metabolite constructions can predict perspective cellular formulas of multipro-/synbiotics for prophylaxis, supporting and accompanying therapy. Approaches developed are universal. They are useful in the study of any Gram-positive and eukaryotic (yeasts and yeast-like fungi) monoand mixed cultures. The methodological principles proposed and described are of value for extended fields of clinical microbiology and medical bio-/nanotechnology.

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Section: Membrane Technological Prospects Of Lssmmentioning

confidence: 99%

Metabolite Multiprobiotic Formulas for Microbial Health

Lakhtin¹,

Lakhtin²,

Алешкин³

et al. 2019

Oral Health by Using Probiotic Products

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“…The equivalent circuit consists of different elements that represent the electrochemical system, e.g., the bulk solution resistance (R s ), the double-layer capacitance (C dl ), the Warburg's impedance (Z w ), and the interfacial electron transfer resistance (R ct ). EIS is a label-free technique commonly applied to detect analytes down to a single-molecule (attomolar) level [63][64][65]. In this context, EIS-based biosensors could be more sensitive electrochemical devices as compared to voltammetric and amperometric biosensors [66].…”

Section: Nanobioengineered Electrochemical Biosensorsmentioning

confidence: 99%

Electrochemical Biosensors for Determination of Colorectal Tumor Biomarkers

et al. 2020

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The accurate determination of specific tumor markers associated with cancer with non-invasive or minimally invasive procedures is the most promising approach to improve the long-term survival of cancer patients and fight against the high incidence and mortality of this disease. Quantification of biomarkers at different stages of the disease can lead to an appropriate and instantaneous therapeutic action. In this context, the determination of biomarkers by electrochemical biosensors is at the forefront of cancer diagnosis research because of their unique features such as their versatility, fast response, accurate quantification, and amenability for multiplexing and miniaturization. In this review, after briefly discussing the relevant aspects and current challenges in the determination of colorectal tumor markers, it will critically summarize the development of electrochemical biosensors to date to this aim, highlighting the enormous potential of these devices to be incorporated into the clinical practice. Finally, it will focus on the remaining challenges and opportunities to bring electrochemical biosensors to the point-of-care testing.

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“…Currently, those types of receptors are not available and directing antibodies to a certain region comprising both protein and glycan moieties is not an easy task. Meanwhile, mixed antibody (for protein recognition) and lectin (for glycan alteration recognition) ELISA-like assays have been developed [12].…”

Section: Receptors For Glycan Structuresmentioning

confidence: 99%

Post-translational modifications in tumor biomarkers: the next challenge for aptamers?

Díaz-Fernández

Miranda‐Castro

de‐los‐Santos‐Álvarez

et al. 2018

Anal Bioanal Chem

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Advances in proteomics have fueled the search for novel cancer biomarkers with higher selectivity. Differential expression of low abundant proteins has been the usual way of finding those biomarkers. The existence of a selective receptor for each biomarker is compulsory for their use in diagnostic/prognostic assays. Antibodies are the receptors of choice in most cases although aptamers are becoming familiar because of their facile and reproducible synthesis, chemical stability as well as comparable affinity and selectivity. In recent years, it has been reported that the pattern of post-translational modifications, altered under neoplastic disease, is a better predictive biomarker than the total protein level. Among others, abnormal glycosylation is attracting great attention. Lectins and antibodies are being used for identification and detection of the carbohydrate moiety with low level of discrimination among various glycoproteins. Such level of selectivity is critical to bring next-generation biomarkers to the clinic. Aptamers that can be rationally tailored for a certain molecule domain can become the golden receptor to specifically detect aberrant glycosylation at each protein or even at each glycosylation site, providing new diagnostic tools for early detection of cancer. Graphical abstract Aptamers may specifically differentiate normal from aberrant glycoproteins.

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Glycan and lectin biosensors

Cited by 58 publications

References 46 publications

Metabolite Multiprobiotic Formulas for Microbial Health

Metabolite Multiprobiotic Formulas for Microbial Health

Electrochemical Biosensors for Determination of Colorectal Tumor Biomarkers

Post-translational modifications in tumor biomarkers: the next challenge for aptamers?

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