Glycated haemoglobin assays. Approaches to standardization of results

Stott, Anthony; Casson, Ian; Higgins, G

doi:10.1046/j.1464-5491.2001.00462.x

Cited by 21 publications

(13 citation statements)

References 15 publications

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“…There are also some factors which effect refractive index of water solutions of hemoglobin. As reported in [9], the refractive index for intact erythrocytes decreases from 1.65 to 1.55 with the increase of pH value from 2 to 7.4, and increases from 1.55 to 1.65 with the elevation of pH from 7.4 to 13. Furthermore, oxygen saturation of hemoglobin solution also effects refractive index: the difference between refractive indices of Hb and HbO 2 solutions measured by OCT at 800 nm is 0.004 [28].…”

Section: Refractive Index Measurementssupporting

confidence: 65%

Monitoring of blood proteins glycation by refractive index and spectral measurements

2008

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Monitoring of blood glucose and glycated proteins level is an urgent requirement for diabetic patients. The amount of glycated hemoglobin and glycated albumin depends on blood glucose concentration and reflects the mean glycemia. The purpose of this study is to investigate the effect of presence of glucose and glycation of proteins on optical properties of water solutions of hemoglobin and albumin with different glucose concentrations. We present the results of feasibility study of the refractive index measurements for water solutions of hemoglobin and albumin with glucose by Abbe refractometer. In addition, absorbance spectrum of water solutions of hemoglobin and albumin with different glucose concentrations has been studied. The experimental results show that the changes of optical properties caused by glycation of proteins can be observed by refractive index and spectral measurements. The refractive index measurements can be potentially applied for evaluation of glycated hemoglobin and glycated albumin amount in blood.

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Section: Refractive Index Measurementssupporting

confidence: 65%

Monitoring of blood proteins glycation by refractive index and spectral measurements

2008

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“…Although between methods analytical variability of HbA 1c has largely been addressed by DCCT alignment,42 the biological variability of HBA 1c , including red cell survival, glycation and deglycation37 43 and now ethnicity, is an inherent limitation of using HbA 1c for diagnostic purposes.…”

Section: Discussionmentioning

confidence: 99%

Ethnic differences in glycated haemoglobin between white subjects and those of South Asian origin with normal glucose tolerance: Table 1

Likhari

Gama²

2010

J Clin Pathol

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“…29 Our results using capillary samples, however, are similar to those studies using venous samples 5 probably because WHO diagnostic criteria 1 take account of sample type. Although analytical variability of HbA 1C between methods has largely been addressed by DCCT alignment, 30 the biological variability of HbA 1C remains an inherent limitation of testing HbA 1C.…”

Section: Discussionmentioning

confidence: 99%

Does HbA_1C predict isolated impaired fasting glycaemia in the oral glucose tolerance test in subjects with impaired fasting glycaemia?

et al. 2008

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Background: To assess the usefulness of erythrocyte glycated haemoglobin (HbA 1C ) as a screening tool to identify those subjects with impaired fasting glycaemia (IFG) who do not have impaired glucose tolerance (IGT) or diabetes mellitus (DM) on a 75 g oral glucose tolerance test (OGTT). Design and methods: All subjects undergoing an OGTT had HbA 1C measured at baseline. Receiver operator characteristics analysis was used to identify optimal HbA 1C cut-off values for diagnosing and excluding IGT and DM. Results: We studied 140 subjects (69 women) with IFG (fasting capillary plasma glucose between 6.1 -6.9 mmol/L). Using World Health Organisation criteria, 27 had isolated IFG, 56 had IGT and 57 had DM. HbA 1C was higher (P , 0.001) in patients with DM (6.8 + 0.93%) when compared with those with IGT (6.3 + 0.68%) and isolated IFG (6.2 + 0.30%), but HbA 1C was similar in those with IGT and isolated IFG. There was no HbA 1C cut-off value differentiating isolated IFG from IGT or DM. None of the subjects with isolated IFG had HbA 1C concentration of .6.8%, but 76% and 54% subjects with IGT and DM, respectively, had HbA 1C of 6.8%. Conclusions: HbA 1C measurement is of limited value in differentiating isolated IFG, IGT and DM in subjects with IFG. It cannot be used to identify which subjects with IFG do not require an OGTT.

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Glycated haemoglobin assays. Approaches to standardization of results

Cited by 21 publications

References 15 publications

Monitoring of blood proteins glycation by refractive index and spectral measurements

Monitoring of blood proteins glycation by refractive index and spectral measurements

Ethnic differences in glycated haemoglobin between white subjects and those of South Asian origin with normal glucose tolerance: Table 1

Does HbA_1C predict isolated impaired fasting glycaemia in the oral glucose tolerance test in subjects with impaired fasting glycaemia?

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Glycated haemoglobin assays. Approaches to standardization of results

Cited by 21 publications

References 15 publications

Monitoring of blood proteins glycation by refractive index and spectral measurements

Monitoring of blood proteins glycation by refractive index and spectral measurements

Ethnic differences in glycated haemoglobin between white subjects and those of South Asian origin with normal glucose tolerance: Table 1

Does HbA1C predict isolated impaired fasting glycaemia in the oral glucose tolerance test in subjects with impaired fasting glycaemia?

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Does HbA_1C predict isolated impaired fasting glycaemia in the oral glucose tolerance test in subjects with impaired fasting glycaemia?