Glycated hemoglobin and lipid peroxidation in erythrocytes of diabetic patients

Cholesterol oxides as biomarkers of oxidative stress in type 1 and type 2 diabetes mellitus

“…Different from a previous report [18] showing that 7-ketocholesterol is the main oxysterol in blood plasma and erythrocytes from patients with DM2, we observed important increments of 7β-hydroxycholesterol, 7α-hydroxycholesterol, cholesterol-β-epoxide and cholesterol-α-epoxide, but not of 7-ketocholesterol, in the blood plasma from both type 1 and type 2 diabetic patients. Our data are consistent with data by Murakami et al [38], which showed a high percentage of the total cholesterol predominantly oxidized at the position 7 (7α-hydroxycholesterol, 7β-hydroxycholesterol and 7-ketocholesterol) in the blood plasma of DM2 subjects.…”

“…Moreover, the positive correlation between some ChOx and fructosamine and glycated hemoglobin suggests that their generation is related to the intensity of glycation and/or glycoxidation. In fact, in vitro studies have shown that incubation of erythrocytes with elevated levels of glucose results in increased membrane lipid peroxidation [18]. Advanced glycation end products (AGES) can also stimulate the formation of reactive oxygen species by a receptormediated process and inhibition of lipid peroxidation also prevented AGE formation [3].…”

Prevention of early renal disease, dyslipidaemia and lipid peroxidation in STZ-diabetic rats by LR-9 and LR-74, novel AGE inhibitors

“…Evidence in both experimental and clinical studies suggests that hyperglycaemia-induced oxidative stress could play a major role in the lipid metabolism in diabetes. Both glucose oxidation and glycation can catalyze PUFA peroxidation of cell membranes [20,37,38], and, in high glucose-environment, proteins and lipoproteins trapped within tissues can undergo glycation to produce ROS and lipid peroxidation products [39]. However, based on the results of our study, hyperglycaemia alone might have only limited influence the levels of lipid peroxidation as both untreated and LR-treated diabetic rats showed no difference in glucose and HbA 1c concentrations.…”

“…In addition, numerous studies have shown that there is an increased formation of lipid peroxidation products in plasma and tissues of diabetic patients [18][19][20], and this increased oxidative stress has been postulated as a major contributing factor for the pathogenesis of various diabetic complications. Recently, it was proposed that increased lipid peroxidation and AGE/ALE formation, possibly catalyzed by hyperglycaemia and oxidative stress, could be the mechanistic link between dyslipidaemia and diabetic complications [21,22].…”

Myoglobins: the link between discoloration and lipid oxidation in muscle and meat

“…[74][75][76] Glycation of Mb was recently demonstrated to enhance the prooxidative activity of Mb and to increase release of free iron ion 81 Another study showed that NO protects cardiomyocytes against tert-butyl hydroperoxide-induced formation of non-protein and proteincentered free radical species and concomitant peroxidation of membrane phospholipids, and NO was concluded to be an important antioxidant in heart tissue. 82 In contrast, NO formation during ischemia reperfusion of hearts isolated from wild-type or NOS knock-out mice showed a contribution of NO to oxidative injury, most likely due to interaction with O 2…”