Glycated hemoglobin (HbA1c) affinity biosensors with ring-shaped interdigital electrodes on impedance measurement

Hsieh, Ku Meng; Lan, Kung Chieh; Hu, Wei; Chen, Ming Kun; Jang, Ling Sheng; Wang, Min Haw

doi:10.1016/j.bios.2013.05.059

Cited by 43 publications

(23 citation statements)

References 30 publications

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“…Authors measured the impedance variation by CV and EIS on the IDEs. Hsieh et al 62 assembled a biosensor with ring-shaped IDEs to promote an uniform distribution and an efficient immobilization of glycated hemoglobin (HbA1c).…”

Section: Electro-immuno Sensorsmentioning

confidence: 99%

Electro-Immuno Sensors: Current Developments and Future Trends

Barbosa¹,

Segura²,

Osma³

2017

IJBSBE

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Abbreviations: Iot, internet of things; ELISA, enzyme-linked immuno sorbent assay; WB, western blot; IHC, immunohistochemistry; ICC, immunocytochemistry; FACS, fluorescence-activated cell sorting; IP, immuno precipitation; ELISPOT, enzyme-linked immuno spot; PSA, prostate specific antigen; IDEs, interdigitated electrodes; HPV, human papilloma virus IntroductionBiosensors have been mostly used in the food industry, medical and biological fields, among others, where physical and chemical sensors cannot accurately measure all variables. Biosensors are usually comprised by a biological recognition element and a transducer.1,2 Among the different commercial biosensors, the most common recognition elements are DNA, 3 enzymes, 4 aptamers 5,6 and antibodies.7,8 DNA-based biosensors detect specific analytes by their cleavage and ligation to a functionalized DNA strand.9 Enzymatic biosensors immobilize an enzyme to an electrode to facilitate the transport of electrons from the enzyme to the electrode, produced by specific reactions at the active site of the enzyme.2 Aptamers are chemically synthesized oligonucleotides 10 that provide high specificity and sensitivity towards a specific target. Antibody-based biosensors recognize a specific antigen from the immobilization of a monoclonal or polyclonal antibody.8 In general, transducers vary according to the end user towards which the sensor is thought. Among others, transducers include electrochemical responses, mass changes, optical absorption or transmission, and thermal readings. During the last decades, several immunoassay techniques have appeared as a response to the need of having specific and sensitive tests for antigen recognition. The methods by which the recognition is perform varies from one to another, but the presence of at least one recognition antibody remains as a constant. Among others, the most common immune tests used in laboratories and medical facilities are the enzyme-linked immuno sorbent assay (ELISA), western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), fluorescence-activated cell sorting (FACS), immuno precipitation (IP), and enzyme-linked immuno spot (ELISPOT). Figure 1 shows a general diagram of these immunoassays.ELISA is a widely used technique for the recognition of antibodies' antigens within a sample by means of the biochemical recognition of an enzyme.11 In this technique, specific antibodies are conjugated to an enzyme that catalyzes a colorimetric molecule. Spectrophotometric measurements are used to determine the antigen concentration in the sample. ELISA tests can be performed in a qualitative way, in which the results are positive or negative by statistical methods; or quantitative, in which a standard curve from spectrophotometric measurements can be used to quantify the concentration of the antigen. 12WB is a technique for the detection of specific proteins in a sample through the electrophoretic transfer from a separating gel to a blotting matrix.13 WB uses available monoclonal or polyclonal antibodies for the d...

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Section: Electro-immuno Sensorsmentioning

confidence: 99%

Electro-Immuno Sensors: Current Developments and Future Trends

Barbosa¹,

Segura²,

Osma³

2017

IJBSBE

View full text Add to dashboard Cite

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“…For example, Song and Yoon manipulated a boronic acid-modified electrode with a GOx (glucose oxidase) backfilling assay to quantitatively measure HbA1c [61]. Utilizing the biomimetic nature of boronic acid, Hsieh et al also fabricated an affinity biosensor to measure HbA1c concentrations based on impedance [62]. Using a low-cost and low-volume sample, it was claimed to be feasible to develop the proposed HbA1c biosensor prototype into a sensitive point-of-care device [62].…”

Section: New Ideas For Hba1c Tests: Glycated Hemoglobin Biosensorsmentioning

confidence: 99%

“…Utilizing the biomimetic nature of boronic acid, Hsieh et al also fabricated an affinity biosensor to measure HbA1c concentrations based on impedance [62]. Using a low-cost and low-volume sample, it was claimed to be feasible to develop the proposed HbA1c biosensor prototype into a sensitive point-of-care device [62]. However, because boronic acid binds to the cis-diol of any glycan moiety (HbA1c is only one of the glycan moieties), studies attempting to fabricate HbA1c biosensors should be cautious to not compromise specificity by using the cost-effective boronic acid in place of antibodies.…”

Section: New Ideas For Hba1c Tests: Glycated Hemoglobin Biosensorsmentioning

confidence: 99%

Current aspects in hemoglobin A1c detection: A review

Ang

Thevarajah

Alias

et al. 2015

Clinica Chimica Acta

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Type 2 diabetes mellitus (T2DM) is a pressing health issue that threatens global health and the productivity of populations worldwide. Despite its long-recognized role in diabetes management, glycated hemoglobin (HbA1c) only received WHO endorsement as a T2DM diagnostic tool in 2011. Although conventional plasma-specific tests have long been utilized to diagnose T2DM, the public should be informed that plasma-specific tests are not markedly better than HbA1c tests, particularly in terms of variability and convenience for diagnosing diabetes. In the midst of the debates associated with establishing HbA1c as the preeminent diabetes diagnostic tool, unceasing efforts to standardize HbA1c tests have played an integral part in achieving more efficient communication from laboratory to clinical practice and thus better diabetes care. This review discusses the current status of HbA1c tests in the diagnosis, prevention, treatment and management of T2DM across the globe, focusing on increasing the recognition of glycated hemoglobin variants with effective utilization of different HbA1c methods, updating the current status of HbA1c standardization programs, tapping into the potential of POC analyzers to establish a cost-effective HbA1c test for diabetes care, and inspiring the advancement of HbA1c biosensors for future clinical usage.

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“…These findings suggest that the proposed system provides sufficient sensitivity to measure the concentration of HbA1c within the required clinically relevant range of HbA1c, where 4-6 % is considered normal and covers the clinical reference range of diabetes (6.5-15 %) [3]. Compared with other electrochemical impedance measurements of HbA1c, the proposed membrane-based biosensor provides a wider linear range for assaying HbA1c [29][30][31]. ESM has a distinctive property.…”

Section: Calibration Curve For the Detection Of Hba1cmentioning

confidence: 99%

“…Therefore, for this purpose, the use of electrochemical impedance spectroscopy for investigating chemical characteristics is highly desirable. A technique for selective HbA1c biosensing by impedance spectroscopy has been previously reported to enable the determination of HbA1c concentrations with high sensitivity [29][30][31]. This previously reported technique depended primarily on a self-assembled monolayer (SAM) of thiophene-3-boronic acid (T3BA) on gold electrodes.…”

Section: Introductionmentioning

confidence: 99%

Selective label-free electrochemical impedance measurement of glycated haemoglobin on 3-aminophenylboronic acid-modified eggshell membranes

et al. 2015

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We propose a novel alternative approach to long-term glycaemic monitoring using eggshell membranes (ESMs) as a new immobilising platform for the selective label-free electrochemical sensing of glycated haemoglobin (HbA1c), a vital clinical index of the glycaemic status in diabetic individuals. Due to the unique features of a novel 3-aminophenylboronic acid-modified ESM, selective binding was obtained via cis-diol interactions. This newly developed device provides clinical applicability as an affinity membrane-based biosensor for the identification of HbA1c over a clinically relevant range (2.3 - 14 %) with a detection limit of 0.19%. The proposed membrane-based biosensor also exhibited good reproducibility. When analysing normal and abnormal HbA1c levels, the within-run coefficients of variation were 1.68 and 1.83%, respectively. The run-to-run coefficients of variation were 1.97 and 2.02%, respectively. These results demonstrated that this method achieved the precise and selective measurement of HbA1c. Compared with a commercial HbA1c kit, the results demonstrated excellent agreement between the techniques (n = 15), demonstrating the clinical applicability of this sensor for monitoring glycaemic control. Thus, this low-cost sensing platform using the proposed membrane-based biosensor is ideal for point-of-care diagnostics.

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Glycated hemoglobin (HbA1c) affinity biosensors with ring-shaped interdigital electrodes on impedance measurement

Cited by 43 publications

References 30 publications

Electro-Immuno Sensors: Current Developments and Future Trends

Electro-Immuno Sensors: Current Developments and Future Trends

Current aspects in hemoglobin A1c detection: A review

Selective label-free electrochemical impedance measurement of glycated haemoglobin on 3-aminophenylboronic acid-modified eggshell membranes

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