Glycated Hemoglobin Measurement: Comparison of Three Methods Versus High Performance Liquid Chromatography

Chaila, María Zulema; Viniegra, Matías; Gagliardino, Juan José; Martínez, Alfredo; Bielke, María Gabriela Simesen de; Frusti, Mauro; Monaco, Luis; Salgado, Pablo; Buso, Carlos; González, Claudio; Commendatore, Víctor

doi:10.1177/1932296821997179

Cited by 9 publications

(7 citation statements)

References 15 publications

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“…Ryman spectroscopy analysis method: the structure is relatively complex, and it is not easy to be accepted by patients [24]. Ion chromatography analysis method: its sensitivity, stability and accuracy are poor, and this method is susceptible to interference from other substances in saliva, which affects the detection results [25]. The ultra-micro ultraviolet spectrophotometer method in this study is a highly repeatable method with high sensitivity and high detection accuracy.…”

Section: Discussionmentioning

confidence: 94%

Comparing Glucose Concentration Among Six Saliva Collection Methods In The Newly Developed Saliva Glucose Detection System

Cui¹,

Hangkun²,

Zhu³

et al. 2021

Preprint

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Objective: This study aims to identify the ideal saliva collection technique and propose a newly sensitive approach to achieve daily non-invasive blood glucose monitoring for healthy people. Method: A total amount of six methods were employed for collecting saliva glucose in 20 healthy participants in the morning (9:00), before lunch (11:00), after lunch (14:00) and in the evening (17:00). Ultra-micro ultraviolet spectrophotometer was used to launch a new saliva glucose detection system in measuring the saliva glucose concentration.Results: The newly developed glucose detection system showed a statistically significant linear relationship in the range of 0.1-6 mg/dl with R2 = 0.999. Besides, the parotid saliva glucose concentration was higher than both the sublingual/submandibular saliva and the whole saliva. The parotid glucose concentration fluctuating after meal, and the sublingual/submandibular glucose concentration steady. Conclusions: The detection limit was 1/110 of the average blood glucose concentration of healthy people when fasting, which fully met the sensitivity requirements for detecting the saliva glucose concentration of healthy people. Besides, the collection method was an important factor that affected the saliva glucose concentration. The unstimulated parotid saliva glucose may be easier to reflect blood glucose concentration, which provided a reference for the evaluation of the health status of subjects and the early prediction of diabetes mellitus.

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Section: Discussionmentioning

confidence: 94%

Comparing Glucose Concentration Among Six Saliva Collection Methods In The Newly Developed Saliva Glucose Detection System

Cui¹,

Hangkun²,

Zhu³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Como se mencionó anteriormente, durante las Quintas Jornadas Rioplatenses se estableció en 110 mg/dL el nivel a partir del cual se debería considerar GAA y además, debido a la falta de estandarización del método en la región, se recomendó no utilizar la HbA1c como método de diagnóstico 1 , indicación que posiblemente hoy debería revisarse porque existe evidencia recientemente publicada en conjunto por la SAD y la ALAC en relación a que, al menos en Argentina, se cuenta con laboratorios que utilizan métodos certificados para la medición de HbA1c que podrían brindar resultados confiables a tal efecto 9 .…”

Section: Discussionunclassified

Glucemia en ayunas entre 100 y 109 mg/dL versus prediabetes según hemoglobina glicosilada

Chaila,

Viniegra,

Gagliardino

et al. 2022

Rev. Soc. Argent. Diabetes

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Introducción: algunos estudios han señalado que valores de glucemia en ayunas entre 100 y 109 mg/dL se asocian con frecuencias elevadas de prediabetes cuando el criterio de clasificación son los valores de HbA1c. La Sociedad Argentina de Diabetes (SAD) sostiene a 110 mg/dL como valor a partir del cual se clasifica a un paciente como portador de glucemia en ayunas alterada; la frecuencia de individuos posiblemente clasificados en forma incorrecta, según este criterio, aún no se conoce en la población argentina.Objetivos: establecer la frecuencia con que se presenta prediabetes según HbA1c en una población sin diagnóstico de diabetes mellitus (DM) con glucemias en ayunas entre 100 y 109 mg/dL; correlacionar las dos variables y cuantificar la probabilidad de que esto ocurra respecto de otros con glucemias en ayunas <100 mg/dL.Materiales y métodos: se incluyeron 1.002 muestras de igual número de sujetos desde 45 laboratorios de análisis clínicos de la Asociación de Laboratorios de Alta Complejidad (ALAC), con procesamiento local de glucemia y centralizado de HbA1c por high performance liquid chromatography (HPLC). Análisis estadístico: chi cuadrado, odds ratio, coeficiente de correlación y determinación de Pearson, y correlación serial de Durbin-Watson.Resultados: frecuencia de HbA1c ≥5,7% en la población estudiada con glucemias de ayunas entre 100 y 109 mg/dL=29,7%; test de chi cuadrado: p<0,001; odds ratio de tener HbA1c ≥5,7% entre la población con glucemias en ayunas de 100 a 109 mg/dL vs aquella con valores <100 mg/dL=4,328 (IC 95% 2,922-6,411); r=0,852, r2 = 0,727, Durbin-Watson=1,152.Conclusiones: la prediabetes diagnosticada por HbA1c resultó cuatro veces más frecuente en la población estudiada con glucemias en ayunas entre 100 y 109 mg/dL, que en aquella con valores por debajo de 100 mg/dL.

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“…Hypertension was defined as systolic blood pressure ≥140 mmHg, diastolic blood pressure ≥90 mmHg, or taking hypertension medications 10 . Diabetes was diagnosed when HbA1c was ³6.5% or using diabetes medications 11 . Dyslipidemia was established if the participant was using hypolipidemic medication or if at least one of the following criteria was met: reduced high-density lipoprotein cholesterol, a level <40 mg/dL in men or 50 mg/dL in women; hypertriglyceridemia, a triglyceride level >150 mg/dL; and hypercholesterolemia, an low-density lipoprotein cholesterol >160 mg/dL 12 .…”

Section: Clinical Parameters and Laboratory Testingmentioning

confidence: 99%

Urbanization and kidney dysfunction in Brazilian indigenous people: a burden for the youth

Gomes

Guimarães

Nicacio

et al. 2023

Rev. Assoc. Med. Bras.

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OBJECTIVE:The aim of this study was to investigate whether the degree of urbanization influences the prevalence of chronic kidney disease in Brazilian indigenous people. METHODS: This is a cross-sectional study conducted between 2016 and 2017 in northeastern Brazil and includes individuals aged between 30 and 70 years from two specific indigenous groups who volunteered to participate in the study: the Fulni-ô people (lowest degree of urbanization) and the Truká group (greater degree of urbanization). Cultural and geographical parameters were used to characterize and measure the magnitude of urbanization. We excluded individuals with known cardiovascular disease or renal failure who required hemodialysis. Chronic kidney disease was defined as a single measurement of an estimated glomerular filtration rate <60 mL/min/1.73 m 2 using the Chronic Kidney Disease Epidemiology Collaboration creatinine equation. RESULTS: A total of 184 indigenous people from the Fulni-ô group and 96 from the Truká group with a median age of 46 years (interquartile range: 15.2) were included. We found a chronic kidney disease rate of 4.3% in the total indigenous population, generally affecting an older population: 41.7% over 60 years old (p<0.001). The Truká people had a chronic kidney disease prevalence of 6.2%, with no differences in kidney dysfunction across age groups. The Fulni-ô participants had a chronic kidney disease prevalence of 3.3%, with a higher proportion of kidney dysfunction in older participants (of the six Fulni-ô indigenous people with chronic kidney disease, five were older). CONCLUSION: Our results suggest that a higher degree of urbanization seems to negatively influence the prevalence of chronic kidney disease in Brazilian indigenous people.

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Glycated Hemoglobin Measurement: Comparison of Three Methods Versus High Performance Liquid Chromatography

Cited by 9 publications

References 15 publications

Comparing Glucose Concentration Among Six Saliva Collection Methods In The Newly Developed Saliva Glucose Detection System

Comparing Glucose Concentration Among Six Saliva Collection Methods In The Newly Developed Saliva Glucose Detection System

Glucemia en ayunas entre 100 y 109 mg/dL versus prediabetes según hemoglobina glicosilada

Urbanization and kidney dysfunction in Brazilian indigenous people: a burden for the youth

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