Glycation inhibitors extend yeast chronological lifespan by reducing advanced glycation end products and by back regulation of proteins involved in mitochondrial respiration

Kazi, Rubina; Banarjee, Reema; Deshmukh, Arati; Patil, Gouri V.; Jagadeeshaprasad, Mashanipalya G.; Kulkarni, Mahesh J.

doi:10.1016/j.jprot.2017.01.015

Cited by 28 publications

(23 citation statements)

References 58 publications

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“…In addition, the down-regulated KEGG pathways were related to the decomposition and utilization of substrates by K. marxianus L1-1. Consistently, a previous report demonstrated that downregulated proteins related to advanced glycation end products were implicated in the aging process [40].…”

Section: Down-regulated Proteins and Genes Indicated The Stable Formasupporting

confidence: 84%

Complementary analyses of transcriptome and proteome revealed the formation mechanism of ethyl acetate, ethanol and organic acids in Kluyveromyces marxianus L1-1 in Chinese fermented acid rice soup

Liu

Miao

Qin

2020

Preprint

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Background: Recently, more chemical and biotechnological applications have been found in Kluyveromyces marxianus than Saccharomyces cerevisiae in the food field because they show advantageous metabolism features in the production of flavor components of interest. However, most of study demonstrated Kluyveromyces marxianus involved in ethanol synthesis in the dairy products in food fields. Our study aims to clarify the formation mechanism of ethyl acetate and organic acids in acid rice soup inoculated with Kluyveromyces marxianus.Results: The higher concentration of ethyl acetate than ethanol and organic acids in fermented acid rice soup inoculated with Kluyveromyces marxianus. Up-regulated genes/proteins, including ADH1, ADH2, ADH6, ATF1, ACCT, and TES1, and down-regulated ALD family involved in glycolysis/gluconeogenesis and pyruvate metabolism played the crucial roles in the formation of ethyl acetate and other esters. In addition, up-regulated genes/proteins involved in starch and sucrose metabolism, amino sugar and nucleotide sugar metabolism, glycolysis/gluconeogenesis, TCA cycle, and pyruvate metabolism played the important roles in the formation of organic acids, ethanol and esters.Conclusion: Our results reveals the formation mechanism of ethyl acetate and organic acids in acid rice soup inoculated with K. marxianus L1-1. This study provides the basis for improving aroma and taste of fermented foods and reveals the formation mechanisms of flavors in no-dairy products.

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Section: Down-regulated Proteins and Genes Indicated The Stable Formasupporting

confidence: 84%

Complementary analyses of transcriptome and proteome revealed the formation mechanism of ethyl acetate, ethanol and organic acids in Kluyveromyces marxianus L1-1 in Chinese fermented acid rice soup

Liu

Miao

Qin

2020

Preprint

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“…By screening an array of 76 gene deletions aged with metformin, we found 419 a number of cases in which metformin buffers both short-or long-lifespan mutant 420 phenotypes. The interacting genes suggest a role of protein glycosylation and protein 421 homeostasis, which is in line with previous evidence showing that metformin alters 422 glycation, protein transport, and protein degradation in yeast(Borklu-Yucel et al, 2015; 423Kazi et al, 2017). Our results also uncovered interactions between metformin and 424 genes involved in mitochondrial function (erp6Δ, lsc2Δ, ema35Δ, and ylh47Δ), which is425 a known player in the cellular response to metformin (Borklu-Yucel et al, 2015; Stynen 426 et al, 2018).…”

supporting

confidence: 82%

“…showing that metformin alters glycation, protein transport, and protein degradation in yeast 24,34 . Our results also uncovered interactions between metformin and genes involved in mitochondrial function (erp6Δ, lsc2Δ, ema35Δ, sap155Δ, and ylh47Δ), which is a known player in the cellular response to metformin 24,25 .…”

Section: Discussionmentioning

confidence: 98%

An optimized competitive-aging method reveals gene-drug interactions underlying the chronological lifespan of Saccharomyces cerevisiae

Avelar-Rivas

Munguía-Figueroa

Juárez-Reyes

et al. 2019

Preprint

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ABSTRACTThe chronological lifespan of budding yeast is a model of aging and age-related diseases. This paradigm has recently allowed genome-wide screening of genetic factors underlying post-mitotic viability in a simple unicellular system, which underscores its potential to provide a comprehensive view of the aging process. However, results from different large-scale studies show little overlap and typically lack quantitative resolution to derive interactions among different aging factors. We previously introduced a sensitive, parallelizable approach to measure the chronological-lifespan effects of gene deletions based on the competitive aging of fluorescence-labeled strains. Here, we present a thorough description of the method, including an improved multiple-regression model to estimate the association between death rates and fluorescent signals, which accounts for possible differences in growth rate and experimental batch effects. We illustrate the experimental procedure—from data acquisition to calculation of relative survivorship—for ten deletion strains with known lifespan phenotypes, which is achieved with high technical replicability. We apply our method to screen for gene-drug interactions in an array of yeast deletion strains, which reveals a functional link between protein glycosylation and lifespan extension by metformin. Competitive-aging screening coupled to multiple-regression modeling provides a powerful, straight-forward way to identify aging factors in yeast and their interactions with pharmacological interventions.

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“…The α -dicarbonyl compounds together with ROS are intermediate products of nonenzymatic glycation/autoxidation of glucose, which participate in aging processes in yeasts [ 16 ]. Higher levels of glycation products are formed at high glucose levels in the medium (2% glucose), where glucose is mainly utilized via fermentation, and it shortens chronological yeast lifespan [ 16 , 36 ]. However, caloric restriction or switch of glucose metabolism from fermentation to respiration may decrease glycation and extend yeast lifespan [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…Higher levels of glycation products are formed at high glucose levels in the medium (2% glucose), where glucose is mainly utilized via fermentation, and it shortens chronological yeast lifespan [ 16 , 36 ]. However, caloric restriction or switch of glucose metabolism from fermentation to respiration may decrease glycation and extend yeast lifespan [ 36 ]. Since above we discussed that AKG may facilitate respiration metabolism in yeast cells when glucose is available at high concentrations, this may contribute to lower DC level in aged yeast cells.…”

Section: Discussionmentioning

confidence: 99%

Effects of Long-Term Cultivation on Medium with Alpha-Ketoglutarate Supplementation on Metabolic Processes ofSaccharomyces cerevisiae

Burdyliuk

Bayliak

2017

Journal of Aging Research

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During last years, alpha-ketoglutarate (AKG), an important intermediate in the Krebs cycle, has been intensively studied as a dietary supplement with stress-protective and potential antiaging effects. Here, we examined the effects of exogenous AKG on metabolic processes and survival of yeast Saccharomyces cerevisiae during long-term cultivation. Growth on AKG had no effect on the total cell number but increased the number of reproductively active cells at the late days of cultivation (from day 7 to day 15). A gradual increase in levels of total protein, glycogen, and trehalose was found over 7-day cultivation with more pronounced effects in AKG-grown cells. In control cells, metabolic activity and the activities of superoxide dismutase and catalase decreased, whereas levels of carbonyl proteins and low-molecular-mass thiols increased during 7-day cultivation. This suggests development of oxidative stress in stationary phase cells. Meanwhile, stationary phase cells cultured on AKG possessed higher levels of low-molecular-mass thiols and lower levels of carbonyl proteins and α-dicarbonyl compounds when compared to control ones. Collectively, higher levels of storage carbohydrates and an activation of antioxidant defense with diminishing oxidative protein damage can prevent a loss of reproductive ability in yeast cells during long-term cultivation on AKG-supplemented medium.

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Glycation inhibitors extend yeast chronological lifespan by reducing advanced glycation end products and by back regulation of proteins involved in mitochondrial respiration

Cited by 28 publications

References 58 publications

Complementary analyses of transcriptome and proteome revealed the formation mechanism of ethyl acetate, ethanol and organic acids in Kluyveromyces marxianus L1-1 in Chinese fermented acid rice soup

Complementary analyses of transcriptome and proteome revealed the formation mechanism of ethyl acetate, ethanol and organic acids in Kluyveromyces marxianus L1-1 in Chinese fermented acid rice soup

An optimized competitive-aging method reveals gene-drug interactions underlying the chronological lifespan of Saccharomyces cerevisiae

Effects of Long-Term Cultivation on Medium with Alpha-Ketoglutarate Supplementation on Metabolic Processes ofSaccharomyces cerevisiae

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