Glyceraldehyde-3-phosphate dehydrogenase from the newt Pleurodeles waltl. Protein purification and characterization of a GapC gene

Purification and partial characterization of glyceraldehyde-3-phosphate dehydrogenase from the ciliate <italic>Tetrahymena thermophila</italic>

“…These data are in agreement with those reported by Zhao et al [23]. A single gapC isoform has been found also in T. pyriformis [25] and other eukaryote and prokaryote organisms [26], but it seems not to be a general rule since the presence of several GAPDH isoforms has been reported in other organisms [27].…”

“…Similar value was obtained for T. pyriformis GAPDH [25]. However, GAPDH from human erythrocyte [29] and Pleurodeles waltl [26] exhibit different values of optimal temperature (40-458C and 408C, respectively) ( Table 2).…”

“…Therefore, the K m NAD þ of T. thermophila GAPDH is clearly lower, suggesting a higher affinity for the nucleotide co-enzyme, as it was observed for T. pyriformis [25] and Camelus dromedarius [30]. For purified GAPDHs from S. pilchardus and P. waltl, the V max (37.6 and 33.24 U/mg, respectively) was similar to that obtained for T. thermophila GAPDH, and the K m NAD þ of S. pilchardus GAPDH [28] was also similar to that estimated for T. thermophila GAPDH, but the K m NAD þ of GAPDH from P. waltl [26] was different. Indeed, the K m D-G3P was similar to those found for T. pyriformis [25] and C. dromedarius GAPDHs [30], but was different from those reported for S. pilchardius [28] and P. waltl GAPDHs [26] ( Table 2).…”

“…The purified T. thermophila GAPDH has a homotetrameric structure. This GAPDH differ in a number of instances from those described previously from other sources [25,26,[28][29][30]. Indeed, the effect of two types of stress (oxidative and nitrosative) on this enzyme was studied, showing that oxidative stress is most toxic than nitrosative stress.…”

Nvbeta-actin and NvGAPDH as normalization factors for gene expression analysis in limb regenerates and cultured blastema cells of the adult newt, Notophthalmus viridescens

“…The resulting forward and reverse primers ( Figure 1B) yielded an expected amplicon of ~510 bp corresponding to the gapC fragment (Mounaji et al, 2002). cDNA synthesis was generated from 2 µg liver total RNA as above.…”