2004
DOI: 10.1074/jbc.m409472200
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Glyceraldehyde-3-phosphate Dehydrogenase Interacts with Rab2 and Plays an Essential Role in Endoplasmic Reticulum to Golgi Transport Exclusive of Its Glycolytic Activity

Abstract: Rab2 requires atypical protein kinase C / (aPKC/) to promote vesicle formation from vesicular tubular clusters (VTCs). The Rab2-generated vesicles are enriched in recycling proteins suggesting that the carriers are retrograde-directed and retrieve transport machinery back to the endoplasmic reticulum. These vesicles also contained the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH). We have previously established that GAPDH is required for membrane transport between the endoplasmic reticulum… Show more

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Cited by 96 publications
(105 citation statements)
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“…GAPDH is an enzyme of glycolysis, but is also involved in eukaryotic membrane fusion events, including fusion of endoplasmic reticulum-derived vesicles with the Golgi apparatus (Glaser & Gross, 1995;Nakagawa et al, 2003;Tisdale, 2001;Tisdale et al, 2004). The addition of GAPDH stimulated killing of E. coli by mxOMVs, suggesting that predatory activity was mediated by fusion with the E. coli membrane, as suggested for OMVs of Pseudomonas aeruginosa (Kadurugamuwa & Beveridge, 1996).…”
Section: A G L Evans and Othersmentioning
confidence: 99%
“…GAPDH is an enzyme of glycolysis, but is also involved in eukaryotic membrane fusion events, including fusion of endoplasmic reticulum-derived vesicles with the Golgi apparatus (Glaser & Gross, 1995;Nakagawa et al, 2003;Tisdale, 2001;Tisdale et al, 2004). The addition of GAPDH stimulated killing of E. coli by mxOMVs, suggesting that predatory activity was mediated by fusion with the E. coli membrane, as suggested for OMVs of Pseudomonas aeruginosa (Kadurugamuwa & Beveridge, 1996).…”
Section: A G L Evans and Othersmentioning
confidence: 99%
“…The beads were washed 4ϫ with 1 ml of buffer A, and the bound proteins were boiled in sample buffer, separated by SDS-PAGE, and then transferred to nitrocellulose. The GST pull-down experiments were performed, as described previously (14). Briefly, BL21 (DE3) pLysS cells (Novagen) that contained the recombinant plasmids GST-GAPDH, GST-Rab2, or GST-Rab1 were grown at 37°C to 0.6 A 600 nm units and then induced with 0.4 mM isopropyl-␤-D-thiogalactopyranoside for 3 h at 37°C.…”
Section: Methodsmentioning
confidence: 99%
“…GAPDH also interacts directly with Rab2 as well as aPKC/ and additionally serves as a substrate for the kinase (13,14). Although GAPDH is essential for membrane trafficking in the early secretory pathway independent of catalytic activity, the precise role of this glycolytic enzyme in transport is unclear (14).…”
mentioning
confidence: 99%
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“…GAPDH is an enzyme that breaks down glucose for energy and carbon molecules (25). This can account for its high COV values in GDS279 and GDS1808, as the mice in the two datasets were given a special diet of high fat or restricted calories, making the expression of GAPDH in the mice liver vary to adapt to the different diets.…”
Section: Discussionmentioning
confidence: 99%