Glyceraldehyde-3-phosphate Dehydrogenase Is Regulated on a Daily Basis by the Circadian Clock

Shinohara, Mari L.; Loros, Jennifer J.; Dunlap, Jay C.

doi:10.1074/jbc.273.1.446

Cited by 82 publications

(49 citation statements)

References 51 publications

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“…GAPDH mRNA is up-regulated in the endometrium, but down-regulated in the liver by estradiol in ewes [Zou and Ing, 1998]. GAPDH mRNA density has also been show to be regulated on a daily basis by the circadian clock [Shinohara et al, 1998]. Beta-actin mRNA expression is also lower in biopsy samples taken from asthmatic lung compared to biopsies from normal lung [Glare et al, 2002].…”

Section: Discussionmentioning

confidence: 99%

Muscarinic receptor transcript and protein density in hypertrophied and atrophied rat urinary bladder

Braverman

Ruggieri

2005

Neurourology and Urodynamics

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Aims-Our previous studies showed that bladder hypertrophy shifts the muscarinic receptor subtype mediating contraction from M 3 towards M 2 along with increased M 2 and decreased M 3 protein concentration. We quantified mRNA for M 1 through M 5 receptors to determine whether the changes in M 2 and M 3 protein levels was due to changes in transcription.Methods-Bladder hypertrophy was induced by bladder outlet obstruction (BOO), major pelvic ganglion electrocautery (DEN), and major pelvic ganglion decentralization (DEC). Bladder atrophy was induced by ureteral diversion (DIV). Additional groups included denervated and diverted (DEN-DIV), sham operated (SHAM), and normal (NOR) controls. Transcripts were quantified using a multiplex ribonuclease protection assay (RPA) and receptor protein density was determined by immunoprecipitation. Receptor transcripts were expressed per unit total RNA.Results-Although all five receptor subtype transcripts were detected in all experimental groups, the densities of M 1 , M 4 , and M 5 were much lower than for the M 2 and M 3 subtype. There were more M 2 receptor transcripts than all the others, consistent with M 2 protein determinations. M 2 transcripts were significantly increased in DEN and BOO bladders. Surprisingly, M 3 transcripts were also significantly increased in BOO. There was a significant correlation (r = 0.98, P < 0.001) between protein density and transcript density for the M 2 but not the M 3 receptor among the different experimental groups.Conclusions-Changes in mRNA concentration are reflected by changes in protein density for the M 2 receptor but not for the M 3 receptor. Extrapolation of functional effects from transcript density data is invalid for M 3 mediated bladder contractions.

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Section: Discussionmentioning

confidence: 99%

Muscarinic receptor transcript and protein density in hypertrophied and atrophied rat urinary bladder

Braverman

Ruggieri

2005

Neurourology and Urodynamics

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“…To date, Ͼ180 ccgs have been identified in N. crassa (10)(11)(12)(13)(14), including genes associated with rhythms in asexual spore development (conidiation; ref. 15), metabolism (16), pheromone production (17), and stress responses (18). However, only a handful of these ccgs have been studied in depth, and few details of the output pathways from N. crassa or any other organism's circadian oscillator are known.…”

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confidence: 99%

Circadian rhythmicity mediated by temporal regulation of the activity of p38 MAPK

Vitalini

Paula

Goldsmith

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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Circadian clocks are composed of central oscillators, input pathways that transduce external information to the oscillators, and output pathways that allow the oscillators to temporally regulate cellular processes. Little is known about the output pathways. In this study, we show that the Neurospora crassa osmosensing MAPK pathway, essential for osmotic stress responses, is a circadian output pathway that regulates daily rhythms in the expression of downstream genes. Rhythmic activation of the highly conserved stress-activated p38-type MAPK [Osmotically Sensitive-2 (OS-2)] by the N. crassa circadian clock allows anticipation and preparation for hyperosmotic stress and desiccation that begin at sunrise. These results suggest a conserved role for MAPK pathways in circadian rhythmicity.circadian output pathway ͉ circadian rhythm ͉ Neurospora crassa ͉ osmotic stress phosphorelay

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“…In higher organisms, one copy of the gene encoding GAPDH is expressed (reviewed [40]) and has been reported to have a variety of activities including apoptosis, nuclear RNA transport, microtubule assembly, protein kinase reactions, and DNA replication [40]. Furthermore, it should be pointed out that the protein level of GAPDH in Neurospora crassa [41] and that of chloroplast GAPDH in the dinoflagellate Gonyaulax polyhedra [42] have been reported to undergo circadian oscillation. On the other hand, yeast contains three members of the GAPDH family, and GAPDH1 has been suggested to have a unique function among the GAPDH proteins, as it is predominantly synthesized in stationary phase or stressed cells [36,37] and alone it cannot support growth [34].…”

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confidence: 99%

Interaction of the GTS1 gene product with glyceraldehyde‐ 3‐phosphate dehydrogenase 1 required for the maintenance of the metabolic oscillations of the yeast Saccharomyces cerevisiae

Liu

Wang

Mitsui

et al. 2002

European Journal of Biochemistry

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We previously reported that GTS1 is involved in regulating ultradian oscillations of the glycolytic pathway induced by cyanide in cell suspensions as well as oscillations of energy metabolism in aerobic continuous cultures. Here, we screened a yeast cDNA library for proteins that bind to Gts1p using the yeast two-hybrid system and cloned multiple TDH cDNAs encoding the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH). We found that the zinc-finger and dimerization sites of Gts1p were required for full ability to bind GAPDH, and Gts1ps mutated at these sites lost the ability to regulate both aerobic and unaerobic ultradian oscillations of energy metabolism.Of the three TDH genes, only TDH1 fluctuated at the mRNA level in continuous culture and its deletion resulted in the disappearance of the oscillation without any affect on growth rate. This loss of biological rhythms in the TDH1-deleted mutant was rescued by the expression of TDH1 but not of TDH2 or TDH3 under the control of the TDH1 promoter. Thus, we hypothesized that Gts1p plays a role in the regulation of metabolic oscillation by interacting with the TDH1 product, GAPDH1, in yeast.Keywords: continuous culture; glyceraldehyde-3-phosphate dehydrogenase; Gts1p; metabolic oscillation; yeast.Ultradian (cycles with a period shorter than 24 h) oscillations of the glycolytic pathway were induced after addition of glucose by inhibiting mitochondrial respiration with cyanide in cell suspensions [1][2][3] or cell extracts [4] of yeast with a periodicity of 1-2 min as monitored by measuring the level of NAD(P)H (reviewed in [5]). The glycolytic pathway has been shown to be an autogenous oscillator under extreme nonequilibrium conditions of energy in dissipative structures, which theoretically include all living organisms [5][6][7]. The pathway oscillates under the primary control of phosphofructokinase [8,9], transferring energy from glucose to NADH, which acts as the feed-forward activator, and then from NADH to ATP, which acts as the feedback inhibitor. After ATP as an inhibitor has been consumed, glucose again begins to enter the glycolytic pathway. Yeast cells also exhibit sustained ultradian oscillations of energy metabolism, with a periodicity of 4 h in continuous (chemostat) culture under aerobic conditions in an open system using a bioreactor [10][11][12][13]. (Hereafter, aerobic oscillation will be referred to as energy metabolism or metabolic oscillation in distinction from cyanide-induced glycolytic oscillation.) Energy-metabolism oscillations, which arise spontaneously under conditions of high cell density ( 5 · 10 8 cellsAEmL )1 ) [14], are detectable as a periodic change in the factors involved in energy metabolism such as dissolved oxygen (DO) levels, CO 2 production, glucose and ethanol concentrations, and amounts of storage carbohydrates [10][11][12][13]. DO oscillation is caused by the periodic change between respiratory and respiratory-fermentative phases, in which oxygen demands are relatively high and low, respectively. Although ...

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Glyceraldehyde-3-phosphate Dehydrogenase Is Regulated on a Daily Basis by the Circadian Clock

Cited by 82 publications

References 51 publications

Muscarinic receptor transcript and protein density in hypertrophied and atrophied rat urinary bladder

Muscarinic receptor transcript and protein density in hypertrophied and atrophied rat urinary bladder

Circadian rhythmicity mediated by temporal regulation of the activity of p38 MAPK

Interaction of the GTS1 gene product with glyceraldehyde‐ 3‐phosphate dehydrogenase 1 required for the maintenance of the metabolic oscillations of the yeast Saccharomyces cerevisiae

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